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2 protocols using k79bb10

1

Immunofluorescence Labeling of Diverse Cellular Markers

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Aquaporin-2, rabbit polyclonal (Sigma); Gamma tubulin, mouse monoclonal clone GTU-88 (Sigma); Arl13b, mouse monoclonal clone N295B/66 (NeuroMab, University of California Davis); T1α, hamster monoclonal clone 8.1.1 (Developmental Studies Hybridoma Bank, University of Iowa); Perilipin-A, goat polyclonal (Abcam, Cambridge, MA); Insulin, guinea pig polyclonal (DAKO); Glucagon, mouse monoclonal clone K79bB10 (Sigma); Helix pomatia agglutinin, Alexa488 conjugated (Thermo Fisher Scientific); Lotus Tetragonolobus Agglutinin, fluorescein labeled (Vector Labs, Burlingame, CA); Arf4, rabbit polyclonal antibody against residues 98–114 of mouse Arf4; Rhodopsin, monoclonal clone 1D4 (Abcam); Peripherin, rabbit polyclonal antibody (Gabriel Travis, University of California Los Angeles); Phosducin, mouse monoclonal clone G-7 (Santa Cruz, Dallas, TX); GM130, mouse monoclonal clone 35 (BD Biosciences, San Jose, CA); B-actin, mouse monoclonal clone C4 (Santa Cruz); Hsp90, rabbit polyclonal (Santa Cruz).
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2

Immunohistochemical Analysis of Pancreatic Markers

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Antibodies used in the study include mouse monoclonal anti-MDM2 (SMP14, Santa Cruz, 1:50 for immunostaining), rabbit polyclonal anti-p53 (fl393, Santa Cruz, 1:100 for immunostaining), mouse monoclonal anti-p53 (NCL-p53–505, Novocastra, 1:50 for immunostaining), rabbit polyclonal anti-Insulin (ab63820, Abcam, 1:200 for immunostaining), mouse monoclonal anti-Glucagon (K79Bb10, Sigma, 1:200 for immunostaining), rabbit polyclonal anti-Pdx1 (PRB-278P, Convance, 1:200 for immunostaining), mouse monoclonal anti-Ki67 (BD, 1:100 for immunostaining), and so on. The fluorescein In situ cell death detection kit was purchased from Roche.
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