Cyto id autophagic detection kit

The following reagents were used: Abs against LC3, ATG5, BAX, Bcl-2, caspase-8, and β-actin were purchased from Proteintech (CA, USA). Abs against JNK, phosphor-JNK, p38, phosphor-p38, ERK1/2, phosphor-ERK1/2, P62/SQSTM1 and cleaved caspase-3 were purchased from Cell Signaling Technology (Boston, USA). Abs against caspase-9 and poly (ADP-ribose) polymerases (PARP) were purchased from Abcam (Cambridge, United Kingdom). Goat anti-mouse IgG (H+L) cross-adsorbed secondary Ab Alexa Fluor 555 was purchased from Invitrogen (Massachusetts, USA). Immunofluorescence reagents were obtained from Zhongshan Golden Bridge Biotechnology (Beijing, China). An Annexin V/PI kit was purchased from KeyGen Biotech (Jiangsu, China). Reactive oxygen species (ROS) and JC-1 kits were purchased from Thermo Fisher Scientific (Massachusetts, USA). CytoID autophagic detection kit was purchased from Enzo Life Sciences. Rapamycin (RAPA), 3-Methyladenine (3-MA), N-acetylcysteine (NAC) and SB-202190 were purchased from MedChemExpress (New Jersey, USA).