Cells were lysed in SDS sample buffer, denatured at 95°C for 10 min, sonicated for 10 min, and lysates were separated by 10% SDS-PAGE. After transfer to nitrocellulose membranes, membranes were blocked in 5% milk (TBS, 0.1% Tween) before incubation with anti-cGAS (1:1,000; Sigma or 1:1,000; Cell Signaling), anti-STING (1:5,000; R&D Systems or 1:1,000; Cell Signaling), anti-IFI16 (1:2,000; Santa Cruz), anti-P-IRF3 (1:2,000; Cell Signaling), or anti-IRF3 (1:2,000; Cell Signaling) antibodies over night or anti-β-Actin-Peroxidase (1:50,000; Sigma-Aldrich) antibody for 1 h. After 2 h of incubation with secondary goat anti-rabbit-HRP, and goat anti-mouse-HRP (KPL), or goat anti-mouse IgG1-HRP (Southern Biotech) membranes were developed with Amersham ECL Western Blotting Detection Reagent (GE Healthcare). For detection of P-IRF3 SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Scientific) was mixed 1:10 with Amersham ECL Western Blotting Detection Reagent.
Anti ifi16
Manufactured by Santa Cruz Biotechnology
Anti-IFI16 is a primary antibody that recognizes the IFI16 (Interferon Gamma Inducible Protein 16) protein. IFI16 is a member of the PYHIN protein family and functions as a DNA sensor involved in the innate immune response. The Anti-IFI16 antibody can be used to detect and study the expression and localization of the IFI16 protein in various biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Anti ha, by Abcam (2 mentions)
Anti icp0, by Santa Cruz Biotechnology (2 mentions)
Anti α tubulin, by Santa Cruz Biotechnology (2 mentions)
Anti rad50, by GeneTex (2 mentions)
Rabbit anti gfp, by Abcam (2 mentions)
Anti slfn5, by Merck Group (2 mentions)
Anti slfn11, by Novus Biologicals (2 mentions)
Mouse anti gfp, by Merck Group (2 mentions)
Anti icp8, by Santa Cruz Biotechnology (2 mentions)
Anti tk, by Santa Cruz Biotechnology (2 mentions)
Anti vp21, by Santa Cruz Biotechnology (2 mentions)
Anti ie, by Santa Cruz Biotechnology (2 mentions)
Anti rnap 2, by Santa Cruz Biotechnology (2 mentions)
Anti dna pkcs, by Santa Cruz Biotechnology (2 mentions)
Anti atrx, by Santa Cruz Biotechnology (2 mentions)
Anti ku70, by Abcam (2 mentions)
Anti v5, by Santa Cruz Biotechnology (2 mentions)
Anti histone h3, by Abcam (2 mentions)
Anti sumo2 3, by Abcam (2 mentions)
Anti β actin, by Merck Group (2 mentions)
4 protocols using anti ifi16
1
Western Blot Analysis of Immune Signaling Proteins
2
Comprehensive Antibody Characterization Protocol
3
Western Blot and Immunofluorescence Antibody Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The following antibodies were used in Western blotting and immunofluorescence studies: anti-IFI16 (mouse) and anti-BZLF1; Zebra (mouse) (Santa Cruz Biotechnology, Inc., Santa Cruz, CA); EBV Ea-D p52/p50 and anti-IFI16 (rabbit) (Millipore, Billerica, MA); mouse monoclonal D.1.17.G38 (against gp350/220) was generated in the laboratory [21 (link)]. Secondary anti-rabbit-IgG and anti-mouse-IgG antibodies linked to horseradish peroxidase, Alexa Fluor-488, and Alexa Fluor-594 were from KPL, Inc., Gaithersburg, MD, or Molecular Probes, Eugene, OR. 12-O-tetradecanoyl-phorbol-13-acetate (TPA) and phosphonoacetic acid (PAA) were purchased from Millipore-Sigma (Billerica, MA). TGF-β was purchased from Sigma-Aldrich (St. Louis, MO).
4
Antibody Characterization for SLFN5, SLFN11, and DNA Repair
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The following antibodies were used: anti-SLFN5 (Sigma-Aldrich; Cat.HPA017760; Lot.B96361), anti-SLFN11 (Novus Biologicals; Cat.NBP1-92368; Lot.H96783), anti-PML (Bethyl Laboratory; Cat.A301-167A, Santa Cruz; Cat.sc-966; Lot.H1413), anti-IFI16 (Santa Cruz; Cat.sc-8023; Lot.C1312), anti-ATRX (Santa Cruz; Cat.sc-15408), anti-DNA-PKcs (Santa Cruz; Cat.sc-5282; Lot.G280), anti-SUMO2+3 (Abcam; Cat.ab3742; Lot.GR8249-1), anti-RNAP II (Santa Cruz; Cat.sc-56767), anti-GFP rabbit (Abcam; Cat.ab290; Lot.GR3251545-1), anti-GFP mouse (Millipore; Cat.MAB2510; Lot.2512480), anti-RAD50 (GeneTex; Cat.GTX70228; Lot.40186), anti-V5 (Santa Cruz; Cat.sc-271944; Lot.E2217), anti-HA (Abcam; Cat.ab9110; Lot.GR3217183-2), anti-GAPDH (GeneTex; Cat.GTX100118; Lot.42158), anti-α-Tubulin (Santa Cruz; Cat.sc-69969; Lot.DO412), anti-β-Actin (Sigma-Aldrich; Cat.a5441; Lot.064M4789V), anti-KU70 (Abcam; Cat.ab83501; Lot.GR3176811-2), anti-Histone H3 (Abcam; Cat.ab1791; Lot.GR3198176-1), anti-ICP0 (Santa Cruz; Cat.sc-53070; Lot. A0313), anti-ICP8 (gifted from David M. Knipe), anti-TK (Santa Cruz; Cat.sc-28037; Lot.K1813), anti-VP21 and anti-gD (gifted from Gary H. Cohen), anti-IE1/IE2 (Virusys; Cat.P1215; Lot.A1345070), anti-UL44 (Virusys; Cat.ca006-100; Lot.C1034151), adenovirus late protein antibody staining Hexon, Penton and Fiber (gift from James M. Wilson), and anti-DBP (gift from Arnold J. Levine).
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