Hplc grade acetonitrile ch3cn

Deionized water was produced by a Millipore Advantage A10 system (Millipore, Bedford, MA, USA). Chloral hydrate was ordered from KeLong Chemical (Chengdu, China), 90% ethanol (HPLC grade) from Thermo Fisher Scientific (Rockford, IL, USA), sequencing-grade trypsin from Promega (Madison, WI, USA), ammonium formate from Thermo Fisher Scientific (Rockford, IL, USA), and HPLC-grade acetonitrile (CH₃CN) from Thermo Fisher Scientific (Rockford, IL, USA). Isotopically-labeled peptide with 99.8% purity: APGLTQALNTK (13C6, 15N2), and unlabeled peptide with the same sequence with 99.0% purity: APGLTQALNTK, were synthesized by GenScript (Nanjing, China).

Chloral hydrate was purchased from KeLong Chemical (Chengdu, China). Deionized water was purified by a Millipore Advantage A10 system (Millipore, Bedford, MA, USA). An amount of 90% ethanol (HPLC grade) was purchased from Thermo Fisher Scientific (Rockford, IL, USA). Sequencing grade trypsin was obtained from Promega (Madison, WI, USA). Ammonium formate was purchased from Thermo Fisher Scientific (Rockford, IL, USA). HPLC grade acetonitrile (CH3CN) was obtained from Thermo Fisher Scientific (Rockford, IL, USA). Isotopically labeled peptide with 99.8% purity: APGLTQALNTK (13C6, 15N2), and unlabeled peptide with the same sequence with 99.0% purity: APGLTQALNTK, were purchased from GenScript (Nanjing, China).

Twelve batches of MCG and GG were collected from the cultivation areas in Huanren County (41.26°N, 125.36°E; Benxi City, Liaoning Province, China) in September 2017, and the voucher specimens were deposited in our lab. Sixty-six standards of PGCAM were purchased from Shanghai Standard Biotech (Shanghai, China) or isolated from the roots of PGCAM and Panax notoginseng C. A. Mey (Yang et al., 2016b (link)). (Supplementary Table S1). HPLC-grade acetonitrile (CH₃CN) and methanol were purchased from Thermo Fisher Scientific (Fair Lawn, NJ, United States). Formic acid (FA) was obtained from Sigma-Aldrich (Saint Louis, MO, United States). Ultra-pure water was prepared in-house using the Milli-Q™ system (Millipore, Bedford. MA, United States).