Rat tnf α elisa kit

The protein levels of interleukin-1 beta (IL-1β), interleukin-6 (IL-6), interleukin-10 (IL-10) and tumor necrosis factor-α (TNF-α) in the hippocampus were determined using ELISA kits. The ELISA was performed according to the manufacturer’s instructions. Briefly, the hippocampus was collected and homogenized in ice-cold RIPA buffer (89900, Pierce, Waltham, MA, USA). The lysate was centrifugated and the supernatant was collected. The total protein concentrate was measured using the BCA method (23227, Pierce, Waltham, MA, USA). The IL-1β, IL-6, IL-10 and TNF-α protein levels were determined by Rat IL-1β ELISA kit (ab100768, Abcam, Boston, MA, USA), Rat IL-6 ELISA kit (ab100772, Abcam, Boston, MA, USA), Rat TNF-α ELISA kit (ab46070, Abcam, Boston, MA, USA) and Rat IL-10 ELISA kit (ab100765, Abcam, Boston, MA, USA), respectively. The optical density was measured by a microplate reader (xMark^TM, Bio-Rad, Hercules, CA, USA). Finally, the protein levels of inflammatory factors were conducted by normalizing them to total protein levels.