Carbomer 940

The chemical materials used in this study include SPRC (also named ZYZ‐802, Shanghai), carbomer 940 (Macklin, China), sodium carboxymethyl cellulose (Macklin), glycerin (Macklin), sodium hydroxide (Macklin), Tween‐20 (Sigma, USA), Tween‐80 (Macklin), TRIzol reagent (Carlsbad, CA, USA), and phosphate‐buffered saline (PBS) tablets (Gaithersburg, MD, USA). Recombinant bovine bFGF was purchased from Essex Bio‐Technology (Zhuhai, China). Hydrogen peroxide solution was obtained from ANNJET (Shandong, China). Alcohol, n‐butanol, and xylene were purchased from SINOPHARM (Shanghai, China). Masson Tricolor Staining Kit, HE Staining Kit, BioDewax and clear solution, tris‐ethylene diamine tetraacetic acid antigen retrieval solution (Tris‐EDTA), pH = 9.0), bovine serum albumin (BSA), horseradish peroxidase (HRP) conjugated goat anti‐rabbit IgG (H+L), and DAB chromogenic kit were purchased from Servicebio (Wuhan, China). Total RNA was extracted with RNA Extraction Kit was purchased from Solarbio (Beijing, China). The HiFiScript gDNA Removal cDNA Synthesis Kit was obtained from CWBIO (Jiangsu, China). The PerfectStar Green qPCR SuperMix (+Dye I/+Dye II) was purchased from TRANS (Beijing, China).

Carbomer 940 was dissolved in ddH₂O to prepare 1 wt% carbomer gel (CBMG) [21 (link)]. Specifically, 1 g of Carbomer 940 (Macklin Reagent, Shanghai, China) was dissolved in 100 mL of ddH₂O and stirred at 60 °C for 30 min. Then, 0.25% triethanolamine (Adamas, Shanghai, China) was added into the carbomer solution to adjust the final pH to 6.5–7.5. After stirring for 10 min, CBMG was prepared. Afterwards, drugs (Rif, Van, and Que) in powders of different weights were mixed with CBMG under stirring for 30 min and left overnight at room temperature to form combined hydrogels. The crude hydrogels were washed three times with ddH₂O to remove the uncombined material and stored in a sealed bottle at 4 °C.

Cryptotanshinone (Lot number 17071601, purity ≥98%, high-performance liquid chromatography; HPLC; Baoji Herbest Bio-Tech Co., Ltd., Baoji, China), carbomer 940 (Macklin Biochemical Co., Ltd., Shanghai, China), glycerin (Aladdin Chemical Reagent Co., Ltd., Shanghai, China), pentobarbital (Sigma, United States), oleic acid (Macklin Biochemical Co., Ltd., Shanghai, China), other chemical substances were analytically pure (AR).