Log In Sign up
The largest database of trusted experimental protocols

Bibr 1532

Manufactured by Bio-Techne
Visit Supplier
Sourced in Israel

BIBR-1532 is a small molecule compound that serves as a potent and selective inhibitor of the CDK9 protein kinase. It functions by inhibiting the activity of CDK9, which plays a crucial role in the regulation of transcription elongation.

Automatically generated - may contain errors

Lab products found in correlation

Costunolide, by Bio-Techne (2 mentions) Pi 828, by Bio-Techne (2 mentions) Gsk 690693, by Bio-Techne (2 mentions) Mitopy1, by Cayman Chemical (2 mentions) Mitosox, by Thermo Fisher Scientific (2 mentions) Rapamycin, by Bio-Techne (1 mentions) Kc7f2, by Bio-Techne (1 mentions) Bevacizumab, by Roche (1 mentions) Avastin, by Roche (1 mentions) Mtor inhibitor rapamycin, by Bio-Techne (1 mentions) Bafilomycin a1 bafa1, by Merck Group (1 mentions) Trichostatin a, by Cayman Chemical (1 mentions)

6 protocols using bibr 1532

1

Targeting Tumor Angiogenesis and Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were treated with Bevacizumab (Avastin®), provided by Hoffmann-La Roche Ltd. (Switzerland), at 5 ng/ml and 100 μg/ml, telomerase inhibitors BIBR-1532 (10 μM) and costunolide (10 μM), PI3K inhibitor PI 828 (10μM), AKT inhibitor GSK 690693 (100 nM), mTOR inhibitor Rapamycin (200 nM), and HIF-1α inhibitor KC7F2 (40 μM) from Tocris (Tocris Bioscience, United Kingdom).
Mahfouz N., Tahtouh R., Alaaeddine N., El Hajj J., Sarkis R., Hachem R., Raad I, & Hilal G. (2017). Gastrointestinal cancer cells treatment with bevacizumab activates a VEGF autoregulatory mechanism involving telomerase catalytic subunit hTERT via PI3K-AKT, HIF-1α and VEGF receptors. PLoS ONE, 12(6), e0179202.
+ Open protocol
+ Expand
2

Evaluating Inhibitor Effects on Ovarian Cancer

Check if the same lab product or an alternative is used in the 5 most similar protocols
A total of 1×105 SKOV-3, Ovcar-3 and Igrov-1 cells were seeded in 6-well plates. Once they reached 80% confluence, these cells were treated at 37°C for 48 h with the following inhibitors: Telomerase inhibitors BIBR-1532 (5 and 10 µM), costunolide (5 and 10 µM) and MST-312 (1 and 2 µM); PI3K inhibitors PI 828, wortmanin and GSK (10 µM); AKT inhibitor GSK 690693 (100 nM); and mTOR inhibitor rapamycin (200 nM) (all Tocris Bioscience). The negative control corresponded to non-treated cells maintained in the same conditions as treated cells. In order to indicate the concentrations that should be used for each inhibitor, a toxicity test was performed, and the concentrations were chosen according to the highest concentration that has no toxic effect, therefore no effect on cell viability.
Antoun S., Atallah D., Tahtouh R., Assaf M.D., Moubarak M., Ayoub E.N., Chahine G, & Hilal G. (2019). Glucose restriction combined with chemotherapy decreases telomere length and cancer antigen-125 secretion in ovarian carcinoma. Oncology Letters, 19(2), 1338-1350.
+ Open protocol
+ Expand
3

Telomerase and Mitochondrial ROS Modulation

Check if the same lab product or an alternative is used in the 5 most similar protocols
The telomerase modulators BIBR-1532 (Tocris Bioscience) and AGS 499 (Ester Priel, PhD, Israel) and the mitochondrial ROS-detecting dyes MitoPY1 (Cayman chemicals) and mitoSox (life technologies) were prepared in DMSO. ET-1 (Peninsula Laboratories) was prepared in saline with 1% bovine serum albumin. All other chemicals were obtained from Sigma-Aldrich and prepared in distilled water or physiological salt solution. All concentrations represent the final concentrations in the organ bath.
Beyer A.M., Freed J.K., Durand M.J., Riedel M., Ait-Aissa K., Green P., Hockenberry J.C., Morgan R.G., Donato A.J., Peleg R., Gasparri M., Rokkas C.K., Santos J.H., Priel E, & Gutterman D.D. (2016). Critical Role for Telomerase in the Mechanism of Flow-Mediated Dilation in the Human Microcirculation. Circulation Research, 118(5), 856-866.
+ Open protocol
+ Expand
4

Telomerase and Mitochondrial ROS Modulation

Check if the same lab product or an alternative is used in the 5 most similar protocols
The telomerase modulators BIBR-1532 (Tocris Bioscience) and AGS 499 (Ester Priel, PhD, Israel), and the mitochondrial ROS-detecting dyes MitoPY1 (Cayman chemicals) and mitoSox (life technologies) were prepared in DMSO. ET-1 (Peninsula Laboratories) was prepared in saline with 1% bovine serum albumin. All other chemicals were obtained from Sigma-Aldrich and prepared in distilled water or physiological salt solution. Other agents were prepared in distilled water or physiological salt solution. All concentrations represent the final concentrations in the organ bath.
Beyer A.M., Freed J.K., Durand M.J., Riedel M., Ait-Aissa K., Green P., Hockenberry J.C., Morgan R.G., Donato A.J., Peleg R., Gasparri M., Rokkas C.K., Santos J.H., Priel E, & Gutterman D.D. (2016). Critical Role for Telomerase in the Mechanism of Flow-Mediated Dilation in the Human Microcirculation. Circulation Research, 118(5), 856-866.
+ Open protocol
+ Expand
5

Modulation of Arteriolar Autophagic Pathways

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Isolated adipose and atrial arterioles were incubated for 15-20 hours in endothelial cell growth media containing 5% serum (Lonza), containing either vehicle (DMSO 2% by volume), Bafilomycin A1 (BAFA1; Sigma-Aldrich, 100 nmol/L), trichostatin A (TSA; Cayman Chemical, 100 nmol/L), ATG3 siRNA (Ambion, AM16708; 10 nmol/L), trehalose (Research Products International, 10 mmol/L), BIBR 1532 (Tocris Bioscience; 10 umol/L), or AGS 499 (Ester Priel, PhD, Israel; 20 nmol/L). TSA and trehalose were independently used to induce autophagy in arterioles, while BAFA1 and ATG3 siRNA were used to inhibit or prevent completion of autophagy in arterioles. Previous work from our lab has demonstrated that modulation of TERT with the BIBR 1532 and AGS 499 induce a switch in mechanism of dilation in human atrial and adipose arterioles from NO to H2O2, and H2O2 to NO, respectively12 (link). Following incubation, arterioles were cannulated and underwent stimulation of FMD as described above.
Hughes W.E., Chabowski D.S., Ait-Aissa K., Fetterman J.L., Hockenberry J., Beyer A.M, & Gutterman D.D. (2020). Critical interaction between telomerase and autophagy in mediating flow-induced human arteriolar vasodilation. Arteriosclerosis, thrombosis, and vascular biology, 41(1), 446-457.
+ Open protocol
+ Expand
6

Isolation and Culture of CD4+ Cells

Cited 2 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were isolated and cultured as described previously.47 (link) Assessment of CD4+ cell purity is demonstrated in Figure I in the online-only Data Supplement. Splenocytes were cultured in a 24-well plate (2×106 cells/2 mL per well). MACSibead mouse T-cell, CD3 and CD28 antibody coated, expansion beads (Miltenyi 130-093-627) were added to medium as described.47 (link) TA-65 activator (TA65) is a telomerase activator purified from Astragalus membranaceous52 (link) and provided by TA-Science Inc (New York, NY). BIBR 1532 (Tocris Bioscience), a telomerase inhibitor,53 (link) was dissolved in dimethyl sulfoxide and used as the indicated concentration.
Richardson G.D., Sage A., Bennaceur K., Al Zhrany N., Coelho-Lima J., Dookun E., Draganova L., Saretzki G., Breault D.T., Mallat Z, & Spyridopoulos I. (2018). Telomerase Mediates Lymphocyte Proliferation but Not the Atherosclerosis-Suppressive Potential of Regulatory T-Cells. Arteriosclerosis, Thrombosis, and Vascular Biology, 38(6), 1283-1296.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!