Multiplex protein arrays

Manufactured by Bio-Rad

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Multiplex protein arrays are a type of analytical tool used in life science research. They allow for the simultaneous detection and quantification of multiple proteins in a single sample. The core function of this product is to provide a high-throughput platform for protein analysis.

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Lab products found in correlation

Bio plex, by Bio-Rad (2 mentions) Bio plex pro human cytokine 27 plex, by Bio-Rad (2 mentions)

6 protocols using multiplex protein arrays

Multiplex Cytokine Quantification in Sera

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Cytokine concentrations in patients sera were quantified by multiplex protein arrays, according to the manufacturer’s instruction (BioRad Laboratories, USA) as described [13 (link)]. In brief, a 2-laser array reader (Bio-Plex, BioRad Laboratories) simultaneously quantifies all cytokines of interest. Standard curves and concentrations were calculated with Bio-Plex Manager 4.1.1 on the basis of the 5-parameter logistic plot regression formula. Bio-Plex Pro Human Cytokine 27-Plex and Bio-Plex Pro Human Cytokine 27-Plex (BioRad Laboratories) were used to detect IL-1b, IL-1RA, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12(p70), IL-13, IL-15, IL-17, Eotaxin, FGFb, G-CSF, GM-CSF, IFNg, IP-10, MCP-1, MIP-1a, PDGF, MIP-1b, RANTES, TNFa, VEGF, HGF.

Diestelhorst J., Junge N., Schlue J., Falk C.S., Manns M.P., Baumann U., Jaeckel E, & Taubert R. (2017). Pediatric autoimmune hepatitis shows a disproportionate decline of regulatory T cells in the liver and of IL-2 in the blood of patients undergoing therapy. PLoS ONE, 12(7), e0181107.

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Hepcidin-25 and Cytokine Measurement

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For quantitative measurement of hepcidin-25, the bioactive form of hepcidin, we used the Hepcidin ELISA (RE54061) according to the manufacturer’s instructions (IBL, Hamburg, Germany). Cytokine concentrations in patients sera were quantified by multiplex protein arrays, according to the manufacturer’s instruction (BioRad Laboratories, USA) as described [18 (link)]. In brief, a 2-laser array reader (Bio-Plex, BioRad Laboratories) simultaneously quantifies all cytokines of interest. Standard curves and concentrations were calculated with Bio-Plex Manager 4.1.1 on the basis of the 5-parameter logistic plot regression formula.

Taubert R., Hardtke-Wolenski M., Noyan F., Lalanne C., Jonigk D., Schlue J., Krech T., Lichtinghagen R., Falk C.S., Schlaphoff V., Bantel H., Muratori L., Manns M.P, & Jaeckel E. (2017). Hyperferritinemia and hypergammaglobulinemia predict the treatment response to standard therapy in autoimmune hepatitis. PLoS ONE, 12(6), e0179074.

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Multiplex Cytokine Profiling in Patient Sera

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Cytokine concentrations in patient sera were quantified by multiplex protein arrays according to the manufacturer’s instructions (BioRad Laboratories, USA) as described recently^{32 (link)}. In brief, 27-Plex and Bio-Plex Pro Human Cytokine 27-Plex (BioRad Laboratories) combined with HGF were used to detect IL-1β, IL-1RA, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8/CXCL8, IL-9, IL-10, IL12(p70), IL-13, IL-15, IL-17, Eotaxin/CCL11, FGF-β, G-CSF, GM-CSF, IFN-γ, IP-10/CXCL10, MCP-1/CCL2, MIP-1a/CCL3, PDGF, MIP-1b/CCL4, RANTES/CCL5, TNF-α, VEGF, and HGF.

Diestelhorst J., Junge N., Jonigk D., Schlue J., Falk C.S., Manns M.P., Baumann U., Jaeckel E, & Taubert R. (2018). Baseline IL-2 and the AIH score can predict the response to standard therapy in paediatric autoimmune hepatitis. Scientific Reports, 8, 419.

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Multiplex Cytokine Profiling in Mouse Plasma

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Heparinized mouse blood was collected, centrifuged for 4 min at 3000 g, and plasma was stored at -80°C. Plasma cytokine and chemokine concentrations were quantified by multiplex protein arrays, according to the manufacturer’s instructions (BioRad Laboratories, Hercules, CA), and as described in [18 (link)]. Plasma levels of the following cytokines and chemokines were measured: IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-10, IL-12p40, IL-12p70, IL-13, IL-17, CCL11, granulocyte colony-stimulating factor (G-CSF), granulocyte macrophage colony-stimulating factor (GM-CSF), chemokine (C-X-C motif) ligand 1 (CXCL1, KC), CCL2 (MCP-1), CCL3 (MIP-1 alpha), CCL4 (MIP-1 beta), CCL5 (RANTES) and tumor necrosis factor alpha (TNF alpha).
To account for the hemodilution at the end of the procedure, all biochemistry and cytokine values were corrected according to the modified equation described by Roth-Isigkeit et al. [10 (link)] In short, values for clinical chemistry and cytokine concentrations were corrected for using the following formula:

V a l u e (c a l c u l a t e d) = (V a l u e (m e a s u r e d) x H e m a t o c r i t (m e a s u r e d)) / H e m a t o c r i t (b a s e l i n e)

Natanov R., Gueler F., Falk C.S., Kühn C., Maus U., Boyle E.C., Siemeni T., Knoefel A.K., Cebotari S., Haverich A, & Madrahimov N. (2018). Blood cytokine expression correlates with early multi-organ damage in a mouse model of moderate hypothermia with circulatory arrest using cardiopulmonary bypass. PLoS ONE, 13(10), e0205437.

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Plasma Cytokine Profiling Post-Transplant

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Heparinized mouse blood was collected 28 days after transplantation and centrifuged for 4 min at 3000 g, plasma was separated and stored at À80 C. Plasma cytokine and chemokine concentrations were quantified by multiplex protein arrays, according to the manufacturer's instructions (BioRad Laboratories, Hercules, Calif) using a technique originally described by Braun and colleagues. 17

Siemeni T., Knöfel A.K., Ius F., Sommer W., Salman J., Böthig D., Falk C.S., Tudorache I., Haverich A, & Warnecke G. (2019). Transplant arteriosclerosis in humanized mice reflects chronic lung allograft dysfunction and is controlled by regulatory T cells. The Journal of thoracic and cardiovascular surgery, 157(6).

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Plasma Cytokine Profile Analysis

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Heparinized mouse blood was collected 28 days after transplantation and centrifuged for 4 min at 3000 g; plasma was separated and stored at -80°C. Plasma cytokine and chemokine concentrations were quantified by multiplex protein arrays, according to the manufacturer's instructions (BioRad Laboratories, Hercules, CA) using

Siemeni T., Knöfel A.K., Madrahimov N., Sommer W., Avsar M., Salman J., Ius F., Frank N., Büchler G., Jonigk D., Jansson K., Maus U., Tudorache I., Falk C.S., Haverich A, & Warnecke G. (2016). In Vivo Development of Transplant Arteriosclerosis in Humanized Mice Reflects Alloantigen Recognition and Peripheral Treg Phenotype of Lung Transplant Recipients. American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons, 16(11).

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