The level of parasitemia was evaluated by flow cytometry. 50 µl samples taken from the parasite cultures were washed in PBS and incubated 30 min with 1:10,000 SYBR Green I DNA stain (Life Technologies). Since neutrophils have DNA as well, distinguishing neutrophils was done by adding anti‐CD11b‐APC antibody (Biolegend 301309) 1:400 in parallel to the SYBR Green staining. APC+ cells were excluded from the analysis. The fluorescence profiles of infected erythrocytes were measured on CytoFLEX (Beckman Coulter) and analyzed by the CytExpert software.
Anti cd11b apc antibody
Manufactured by BioLegend
Sourced in United States
The Anti-CD11b-APC antibody is a fluorescently labeled monoclonal antibody that specifically binds to the CD11b antigen expressed on the surface of certain immune cells, such as monocytes, macrophages, and granulocytes. This antibody is conjugated to the APC (allophycocyanin) fluorescent dye, allowing for detection and analysis of CD11b-positive cells using flow cytometry or other fluorescence-based techniques.
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2 protocols using anti cd11b apc antibody
1
Measuring Parasitemia by Flow Cytometry
2
Apoptosis and CD11b Expression Assay
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Cells were treated with DMSO, 20 nM, or 50 nM TP-0903 for up to 48 h. At the indicated time points, cells were collected and washed with PBS. DAPI was added to each sample as a viability dye at a final concentration of 0.1 ug/1 mL. For apoptosis assays, cells were incubated with annexin V-APC (BioLegend, San Diego, CA, USA)) according to the manufacturer’s instructions. For analysis of CD11b, CD11b expression was measured using an anti-CD11b-APC antibody (BioLegend, San Diego, CA, USA). Two-color flow cytometry was performed in the OSUCCC Flow Cytometry Shared Resource, and data were analyzed using FlowJo (BD Biosciences).
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