Sm22 alpha

Manufactured by Abcam

SM22 alpha is a protein that is commonly used as a marker for smooth muscle cells. It plays a role in the regulation of actin cytoskeleton dynamics and cell migration.

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Lab products found in correlation

Alexa fluor 488 streptavidin, by Jackson ImmunoResearch (1 mentions) Immu mount, by Thermo Fisher Scientific (1 mentions) Cy3 αsma, by Merck Group (1 mentions) Pab2627, by Hypoxyprobe (1 mentions) B 1205, by Vector Laboratories (1 mentions) Fluoview fv1000, by Zeiss (1 mentions) Lsm 780 confocal microscope, by Zeiss (1 mentions) Ab7260, by Abcam (1 mentions) Sarcomeric α actinin, by Merck Group (1 mentions) Calponin, by Abcam (1 mentions) Texas red affinipure donkey anti mouse igg, by Jackson ImmunoResearch (1 mentions) Laminin, by Abcam (1 mentions) Gata4, by R&D Systems (1 mentions) Fitc affinipure donkey anti rabbit igg h l, by Jackson ImmunoResearch (1 mentions)

2 protocols using sm22 alpha

Retinal Vasculature and Hypoxia Characterization

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Angpt4^Cre; Rosa26^mT/mG mice eyes were fixed in 4% PFA, retinas were dissected and flattened in Immu-Mount (Thermo Scientific) between cover and objective glass for microscopy analysis. For retina immunofluorescent staining, dissected retinas were treated for 1 hr with cold methanol, permeabilized and blocked for 2 hr with 50% BSA–1% Triton–1xPBS. Primary antibody stainings were carried out overnight using Cy3-αSMA (C6198, Sigma-Aldrich), SM22 alpha (ab10135, Abcam), ColIV (AB756P, Merck Millipore), GFAP (ab7260, Abcam), pimonidazole (Pab2627, Hypoxyprobe) and biotinylated isolectin B4 (B-1205, Vector) antibodies, and visualized with Alexa Fluor 488 streptavidin, Alexa Fluor 488-, Alexa Fluor 647- and Cy3-conjugated secondary antibodies (Jackson ImmunoResearch). Retinas were imaged using Olympus FluoView FV1000 or Zeiss LSM780 confocal microscope. Coverage of αSMA-positive cells was quantified from branching point region of two largest veins at peripheral area of retina. The width of the veins was measured just below the venal branching point at peripheral retina or close to optic nerve head for comparison. 5-Bromo-4-chloro-3-indolyl β-D-galactosidase (X-Gal) staining of retinas was performed as described in Gossler and Zachgo, 1994 .

Elamaa H., Kihlström M., Kapiainen E., Kaakinen M., Miinalainen I., Ragauskas S., Cerrada-Gimenez M., Mering S., Nätynki M, & Eklund L. (2018). Angiopoietin-4-dependent venous maturation and fluid drainage in the peripheral retina. eLife, 7, e37776.

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Immunostaining of Pluripotent and Cardiac Cells

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The iPS cells and the cells at day 22–28 of iPS EB differentiation were fixed with 4% paraformaldehyde, and stained following a standard immunostaining procedure. Briefly, cells were fixed in 4% formaldehyde solution in phosphate-buffered saline (PBS), permeated with 5% bovine serum albumin (BSA) plus 0.1 Triton X100, and blocked with 5% BSA plus 0.01% tween 20. The primary and secondary antibodies were diluted in blocking solution. The primary antibodies against mouse Nanog (cat# ab80892, Abcam), Sox2 (cat# ab5603, Millipore), Oct4 (cat# sc-5279, Santa Cruz), Connex43 (cat# C6219, Sigma);α-sarcomeric actinin (cat# A7732, Sigma); MHC (ab15, Abcam); cTnT (ab8295, Abcam); Laminin (cat# ab11575, Abcam); SM22 alpha (cat# ab14106, Abcam); alpha smooth muscle Actin (cat# ab5694,Abcam); Calponin (cat# ab46794, Abcam); GATA4 (cat# AF2606, R&D system); and NKx2.5 (cat# AF2444, R&D system) were used at a 1:200 dilution. Secondary antibodies, Texas Red AffiniPure Donkey Anti-Mouse IgG (cat#715-075-150, Jackson ImmunoResearch), Texas Red AffiniPure Donkey Anti-Goat IgG (H+L) (cat# 705-075-003, Jackson ImmunoResearch), FITC AffiniPure Donkey Anti Rabbit IgG (H+L) (cat# 711-095-152, Jackson ImmunoResearch), and Texas Red AffiniPure Donkey Anti- Rabbit IgG (H+L) (Cat#711-075-152, Jackson ImmunoResearch) were used at a 1:200 dilution.

Wang H., Xi Y., Zheng Y., Wang X, & Cooney A.J. (2016). Generation of Electrophysiologically Functional Cardiomyocytes from Mouse Induced Pluripotent Stem Cells. Stem cell research, 16(2), 522-530.

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