Cytokeratin 18 ck18

Flow cytometry was performed using the FACS Canto II cytometer (BD), and subsequent analysis was performed using the FACS Diva software (BD). Briefly, cells and spheroids resuspended in PBS were pelleted. Fluorescently conjugated primary antibodies α-smooth muscle 1A4 (Abcam), fibroblast monoclonal antibody (D7-FIB, MA5-16642, Invitrogen), CD24, CD234 (E-Cadherin), CD44, CD227, EpCAM (EBA-1), CD49c, CD116, SSEA-1, CD45, CD47, ALDH1A1 (LSBio), cytokeratin 18 (CK18), and SSEA-1 (CD15) were purchased from BD Pharmingen unless otherwise specified. Cells were incubated with antibodies for an hour and then analyzed on the flow cytometer. For the flow cytometry analysis, cells were gated based on FSC and SSC properties, and interval gates on histogram plots were set up for positive cell populations in FITC, PE, and APC-Cy7 channels.

Flow cytometry was performed using the FACS Canto II cytometer (BD) and subsequent analysis was performed using the FACS Diva software (BD). Briefly, 500,000 cells re-suspended in PBS were pelleted. Fluorescently labelled primary antibodies α-smooth muscle 1A4 (Abcam), CD24, CD234 (E-Cadherin), CD44, CD227, EpCAM (EBA-1), HER2, CD49c, CD116, SSEA-1, CD45, CD47, ALDH1 (LSBio), cytokeratin 18 (CK18) and SSEA-1 (CD15) were purchased from BD Pharmingen unless otherwise specified. Cells were incubated with antibodies and analyzed on the flow cytometer. For the flow cytometry analysis, cells were gated based on FSC and SSC properties and Interval gates on histogram plots were set-up for positive cell populations in FITC, PE and APC-Cy7 channels.