The coagulation tests used to screen for HT were PC activity (Stachrom® Protein C, Diagnostica Stago, Asnières, France), PS free Ag (Liatest® Free Protein S, Diagnostica Stago), AT activity (Stachrom® AT III, Diagnostica Stago), and plasminogen activity (Stachrom® Plasminogen, Diagnostica Stago), per the international guidelines [19 (link)]. All coagulation tests were performed on the STA®-Evolution Coagulation Analyzer. Reference ranges were determined according to our institutional data. Whenever possible, coagulation tests were repeated 2 weeks after the discontinuation of anticoagulation therapy if results at the time of diagnosis showed low levels of multiple NAs or if tests with abnormal results were performed under anticoagulant use.
Stachrom protein c
Manufactured by Diagnostica Stago
Sourced in France
Stachrom® Protein C is a laboratory diagnostic instrument used for the quantitative determination of Protein C in human plasma. It provides a standardized and reliable method for the measurement of Protein C levels, which is an important indicator of coagulation and thrombotic disorders.
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2 protocols using stachrom protein c
1
Coagulation Screening for Hereditary Thrombophilia
2
Routine Coagulation Analyses in Citrated Plasma
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Routine coagulation analyses were performed in citrated plasma using a STA‐R Evolution® instrument and reagents from Diagnostica Stago (Asniéres, France). STA® SPA+ reagent was used for prothrombin time‐international normalized ratio (PT‐INR), and STA®‐PPT for activated partial thromboplastin time (APTT; Diagnostica Stago) and clot detection methods. The colorimetric kits STACHROM® protein C and STACHROM® ATIII (Diagnostica Stago) were used for analysis of protein C and anti‐thrombin III, respectively. The clot‐based test kit STACLOT® protein S (Diagnostica Stago) and the COATEST® APCTM Resistance V kit from Chromogenix (Bedford, MA, USA) were used for analysis of protein S and activated protein C (APC) resistance, respectively. FVIII, von Willebrand factor antigen and activity, lupus anti‐coagulant, anti‐cardiolipin IgG and IgM and anti‐beta2‐glycoprotein I IgG and IgM were estimated in blood samples from the C5D individual by routine analyses at the Department of Medical Biochemistry, Oslo University Hospital, Oslo, Norway.
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