Two hundred and fifty-six Han Chinese patients from Shandong province with RA diagnosed according to the 2008 Classification Criteria of the American College of Rheumatology and 331 healthy controls from the same geographic and ethnic background were included in this study. RA patients were recruited from the department of Rheumatology; Provincial Hospital affiliated to Shandong University, China. Controls had no history of infectious or chronic inflammatory autoimmune diseases and were unrelated to the patients. The study was approved by the Local Ethical Committee of the hospital and university, and informed consent was obtained from all subjects. The methods were carried out in accordance with the approved guidelines. Laboratory parameters were recorded including Rheumatoid factor (RF), anti-cyclic citrullinated peptide antibody (aCCP), anti-Glucose phosphate isomerase (aGPI), erythrocyte sedimentation rate (ESR ) and C-reactive protein (CRP). RF was measured using N Latex RF Kit (SIEMENS, Germany). The level of aCCP and aGPI were determined by enzyme linked immunosorbent assay (ELISA) (AESKULISA, Germany). ESR was determined by the Westergren method via a Monitor-100 machine (BALZELLA, Italy) and CRP by immune turbidimetric analysis (SIEMENS, Germany).
N latex rf kit
Manufactured by Siemens
Sourced in Germany, United States
The N Latex RF Kit is a laboratory equipment product designed for the quantitative determination of rheumatoid factors (RF) in human serum or plasma samples. It utilizes a latex-enhanced immunoturbidimetric assay method to measure RF levels. The kit provides the necessary reagents and materials to perform the RF analysis.
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11 protocols using n latex rf kit
1
Rheumatoid Arthritis Biomarkers in Chinese Patients
2
Retrospective Serum Biomarkers for TNFi
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Serum samples were collected at time of diagnosis and prior TNFi initiation in order to perform other determinations as part of laboratory routine, and then were stored at −20°C. Serum RF and ACPA from patients who initiated TNFi at late 90's and early 2000's were determined a posteriori. Serum RF concentrations were measured by nephelometry using the BNII System and N Latex RF Kit (Siemens Healthcare Diagnostics Inc., Newark, USA) according to the instruction manual. RF > 20 IU/mL was considered positive. Serum ACPA was determined by ELISA as anti-CCP2 with the ImmunoscanCCPlus kit (Svar, Malmö, Sweden). ACPA > 25 IU/mL was considered positive. RF and ACPA seropositivities were confirmed at least in two determinations (at time of diagnosis and prior TNFi initiation).
3
Rheumatoid Factor and ACPA Measurement
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Rheumatoid factor was detected by N-latex RF kit (Siemens Healthcare Diagnostics, München, Germany) or N-Assay LA RF-K Nittobo (Nittobo Medical, Koriyama, Japan), and IgG rheumatoid factor was measured by Smitest IgG RF ELISA (Medical & Biological Laboratories Co., Ltd., Nagoya, Japan). Anti-citrullinated peptide antibody was measured by Mesacup-2 test CCP (Medical & Biological Laboratories).
4
Quantifying Serum Biomarkers in TNFi Therapy
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Serum BAFF concentrations were measured at the baseline and at 6 months of TNFi treatment using the BAFF Human Instant ELISA Kit (R&D Systems, Minneapolis, MN, USA) following the manufacturer’s instructions. Serum RF concentrations were measured by nephelometry using the BNII System and N Latex RF Kit (Siemens Healthcare Diagnostics Inc., Newark, DE, USA) according to the instruction manual. Serum ACPA was determined by ELISA as anti-CCP2 with the Immunoscan CCPlus kit (Svar, Malmö, Sweden).
5
Quantifying Plasma RF and IgG
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Plasma RF concentrations were quantitated using the BNII System and N Latex RF
Kit (Siemens Healthcare Diagnostics Inc., Newark, USA). IgG concentrations were
determined using the BNII System and N Latex IgG Kit (Siemens Healthcare
Diagnostics Inc.) according to the manufacturer’s instructions.
Kit (Siemens Healthcare Diagnostics Inc., Newark, USA). IgG concentrations were
determined using the BNII System and N Latex IgG Kit (Siemens Healthcare
Diagnostics Inc.) according to the manufacturer’s instructions.
6
Serum Autoantibody Profiling in Rheumatoid Arthritis with or without Interstitial Lung Disease
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Sera from the 34 RA patients with or without ILD were collected, and these individual sera were analyzed for anti-HLA Ab profiles.
Sera from the 15 collagen disease patients with AoDILD were collected on admission and in the stable state, at least three months before admission. Two samples were collected from each patient. These individual sera were analyzed for anti-HLA Ab profiles. The sera from these patients either with AoDILD or in the stable state were combined; the two pooled sera at these two states were screened for the auto-Ab profiling to be described in the following sections (Fig. 1 ).
Rheumatoid factor and anti-citrullinated peptide antibody were detected using N-latex RF kit (Siemens Healthcare Diagnostics, Germany) and Mesacup-2 test CCP (Medical & Biological Laboratories, Japan), respectively. KL-6 and SP-D were detected using a Picolumi KL-6 Electrochemiluminescence immunoassay system (EIDIA Co., Ltd, Japan) and SP-D kit “Yamasa” EIA II (Yamasa Corporation, Japan), respectively.
Sera from the 15 collagen disease patients with AoDILD were collected on admission and in the stable state, at least three months before admission. Two samples were collected from each patient. These individual sera were analyzed for anti-HLA Ab profiles. The sera from these patients either with AoDILD or in the stable state were combined; the two pooled sera at these two states were screened for the auto-Ab profiling to be described in the following sections (
Rheumatoid factor and anti-citrullinated peptide antibody were detected using N-latex RF kit (Siemens Healthcare Diagnostics, Germany) and Mesacup-2 test CCP (Medical & Biological Laboratories, Japan), respectively. KL-6 and SP-D were detected using a Picolumi KL-6 Electrochemiluminescence immunoassay system (EIDIA Co., Ltd, Japan) and SP-D kit “Yamasa” EIA II (Yamasa Corporation, Japan), respectively.
7
Rheumatoid Factor and Anti-CCP Antibody Measurement
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During the first visit (baseline), RF levels were measured through nephelometry by using the N Latex RF Kit from Siemens Healthcare Diagnostics Products, Marburg, Germany). Additionally, anti-CCP antibodies (Quanta Lite CCP3 IgG ELISA kit; Inova Diagnostics, Inc., San Diego, CA, USA) were examined twice in 12 months (at baseline and month 12).
8
Serum MCP-1 Levels in Rheumatoid Arthritis
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Serum MCP-1 from RA patients was examined in duplicate by ELISA assay (mean values taken) in accordance with the manufacturer’s recommendations (R&D systems, Minneapolis, MN, USA). At the first visit, RF (measured by nephelometry with N Latex RF Kit from Siemens Healthcare Diagnostics Products GmbH, Marburg, Germany) and anti–cyclic citrullinated peptide (anti-CCP) antibodies (Quanta Lite CCP3 IgG ELISA kit; Inova Diagnostics, Inc., San Diego, CA, USA) were examined. Normal ranges: MCP-1 (0.0–103.7 pg/mL: mean plus two standard deviations, ref. 17 (link)).
9
Rheumatological Factor and Anti-CCP2 Measurement
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Serum samples were collected at time of diagnosis and prior to anti-TNF initiation in order to perform other determinations as part of routine laboratory practice. Rheumatological factor (RF) concentrations were measured by nephelometry using the BNII System and N Latex RF Kit (Siemens Healthcare Diagnostics Inc., Newark, NJ, USA) according to the instruction manual. Serum anti–citrullinated protein antibody was determined by ELISA as anti-CCP2 using the ImmunoscanCCPlus kit (Svar, Malmö, Sweden).
10
Rheumatoid Arthritis Biomarker Study
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A total of 30 RA patients and 25 controls were enrolled from the Rheumatology Clinics and the Dental Clinics of Kaohsiung Medical University Hospital (KMUH), respectively. They were diagnosed according to the 2010 RA classification criteria [49 (link)]. The exclusion criteria included smokers, those with malignancy or pregnancy, those who were breastfeeding, and those who were under antibiotic treatment within fewer than three months prior to the enrollment. Demographic and behavioral characteristics were collected using questionnaires. The RA patients’ past medical history, diabetes mellitus (DM) statuses, clinical and laboratory data on their RA statuses, and medications were obtained from their medical records. The levels of their serum ACPAs and RF were determined by the Elia CCP kit (Phadia AB, Uppsala, Sweden) and N Latex RF kit (Siemens Healthcare Diagnostics, München, Germany), respectively. The levels of the serum ACPAs and RF from the controls were also determined by the same procedures and technicians as those from the RA patients.
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