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Buthionine sulfoximine

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Buthionine sulfoximine is a chemical compound used in laboratory research. It is a potent inhibitor of the enzyme gamma-glutamylcysteine synthetase, which is involved in the synthesis of the antioxidant glutathione. The primary function of buthionine sulfoximine is to deplete cellular glutathione levels, which can be useful in various experimental settings.

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Lab products found in correlation

Thapsigargin, by Merck Group (4 mentions) Ferrostatin 1, by Merck Group (4 mentions) Monochlorobimane, by Thermo Fisher Scientific (2 mentions) Vincristine, by Selleck Chemicals (2 mentions) Bay11 7085, by Selleck Chemicals (2 mentions) Q vd oph, by Thermo Fisher Scientific (2 mentions) Bortezomib, by Merck Group (2 mentions) Doxorubicin, by Selleck Chemicals (2 mentions) C11 bodipy581 591, by Thermo Fisher Scientific (2 mentions) Bay 11 7821 7082, by Selleck Chemicals (2 mentions) Nec 1, by Abcam (2 mentions) N acetyl l cysteine, by Merck Group (2 mentions) Vinblastine, by Selleck Chemicals (2 mentions) Sorafenib, by Selleck Chemicals (2 mentions) Bazedoxifene, by Selleck Chemicals (2 mentions) Camptothecin, by Selleck Chemicals (2 mentions) Rapamycin, by Thermo Fisher Scientific (2 mentions) Etoposide, by Thermo Fisher Scientific (2 mentions) Raloxifene, by Selleck Chemicals (2 mentions) Bortezomib, by Thermo Fisher Scientific (2 mentions)

4 protocols using buthionine sulfoximine

1

Ferroptosis Inducers and Inhibitors

Cited 2 times
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Erastin2 (compound 35MEW28 in (Dixon et al., 2014 (link))) and ML162 were synthesized by Acme Bioscience (Palo Alto, CA, USA). Bortezomib (Cat# NC0587961), thapsigargin (Cat# T9033), N-Acetyl-L-Cysteine (Cat# A8199) and ferrostatin-1 (Cat# SML0583) were from Sigma-Aldrich. Buthionine sulfoximine (Cat# AC23552-0010), Q-VD-OPh (Cat# OPH00101M) and monochlorobimane (Cat# M-1381MP) were from Thermo Fisher. C11 BODIPY 581/591 (Cat# D3861) was from Molecular Probes (Eugene, OR) and dissolved in methanol. RSL3 (Cat # S8155), Doxorubicin (Cat# S1208), vincristine (Cat# S1241), BAY-11-7085 (Cat# S7352) and BAY-11-7821/7082 (Cat# S2913) were from Selleck Chemicals (Houston, TX). Nec-1s (Cat# 2263) was from BioVision (Milpitas, CA). Buthionine sulfoximine was dissolved directly into cell media. All other compounds were prepared as stock solutions in DMSO and stored prior to use at −20°C. Cyst(e)inase enzyme was purified as described and stored at −80°C until use (Cramer etal., 2017 (link)).
Cao J.Y., Poddar A., Magtanong L., Lumb J.H., Mileur T.R., Reid M.A., Dovey C.M., Wang J., Locasale J.W., Stone E., Cole S.P., Carette J.E, & Dixon S.J. (2019). A Genome-wide Haploid Genetic Screen Identifies Regulators of Glutathione Abundance and Ferroptosis Sensitivity. Cell reports, 26(6), 1544-1556.e8.
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2

Ferroptosis Inducers and Inhibitors

Cited 2 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
Erastin2 (compound 35MEW28 in (Dixon et al., 2014 (link))) and ML162 were synthesized by Acme Bioscience (Palo Alto, CA, USA). Bortezomib (Cat# NC0587961), thapsigargin (Cat# T9033), N-Acetyl-L-Cysteine (Cat# A8199) and ferrostatin-1 (Cat# SML0583) were from Sigma-Aldrich. Buthionine sulfoximine (Cat# AC23552-0010), Q-VD-OPh (Cat# OPH00101M) and monochlorobimane (Cat# M-1381MP) were from Thermo Fisher. C11 BODIPY 581/591 (Cat# D3861) was from Molecular Probes (Eugene, OR) and dissolved in methanol. RSL3 (Cat # S8155), Doxorubicin (Cat# S1208), vincristine (Cat# S1241), BAY-11-7085 (Cat# S7352) and BAY-11-7821/7082 (Cat# S2913) were from Selleck Chemicals (Houston, TX). Nec-1s (Cat# 2263) was from BioVision (Milpitas, CA). Buthionine sulfoximine was dissolved directly into cell media. All other compounds were prepared as stock solutions in DMSO and stored prior to use at −20°C. Cyst(e)inase enzyme was purified as described and stored at −80°C until use (Cramer etal., 2017 (link)).
Cao J.Y., Poddar A., Magtanong L., Lumb J.H., Mileur T.R., Reid M.A., Dovey C.M., Wang J., Locasale J.W., Stone E., Cole S.P., Carette J.E, & Dixon S.J. (2019). A Genome-wide Haploid Genetic Screen Identifies Regulators of Glutathione Abundance and Ferroptosis Sensitivity. Cell reports, 26(6), 1544-1556.e8.
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3

Compound Library Screening for Bioactive Inhibitors

Cited 1 time
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An 1,833-member bioactive compound library, and an independent 86-member PI3K signaling inhibitor library comprising mTOR, PI3K and AKT pathway inhibitors, were obtained from Selleck Chemicals and stored at −80°C. Erastin2, annotated as compound 35MEW28 (Ref.51 (link)) and ML162 were synthesized by Acme Bioscience. Erastin was the kind gift of B. Stockwell (Columbia). Chemical used (supplier) were: DMSO, ferrostatin-1, thapsigargin, tunicamycin, cycloheximide, L-arginine, D-arginine and L-citrulline (Sigma-Aldrich Corporation); bortezomib, rapamycin, etoposide and buthionine sulfoximine (Thermo-Fisher Scientific); INK 128, AZD8055, vinblastine, camptothecin, sorafenib, bazedoxifene, raloxifene and JTC-801 (Selleck Chemicals); and GCN2iB (MedChemExpress). buthionine sulfoximine was dissolved directly into cell media. All other drugs were prepared as stock solutions in DMSO. Stock solutions were stored at −20˚C.
Conlon M., Poltorack C.D., Forcina G.C., Armenta D.A., Mallais M., Perez M.A., Wells A., Kahanu A., Magtanong L., Watts J.L., Pratt D.A, & Dixon S.J. (2021). A Compendium of Kinetic Modulatory Profiles Identifies Ferroptosis Regulators. Nature chemical biology, 17(6), 665-674.
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4

Compound Library Screening for Bioactive Inhibitors

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
An 1,833-member bioactive compound library, and an independent 86-member PI3K signaling inhibitor library comprising mTOR, PI3K and AKT pathway inhibitors, were obtained from Selleck Chemicals and stored at −80°C. Erastin2, annotated as compound 35MEW28 (Ref.51 (link)) and ML162 were synthesized by Acme Bioscience. Erastin was the kind gift of B. Stockwell (Columbia). Chemical used (supplier) were: DMSO, ferrostatin-1, thapsigargin, tunicamycin, cycloheximide, L-arginine, D-arginine and L-citrulline (Sigma-Aldrich Corporation); bortezomib, rapamycin, etoposide and buthionine sulfoximine (Thermo-Fisher Scientific); INK 128, AZD8055, vinblastine, camptothecin, sorafenib, bazedoxifene, raloxifene and JTC-801 (Selleck Chemicals); and GCN2iB (MedChemExpress). buthionine sulfoximine was dissolved directly into cell media. All other drugs were prepared as stock solutions in DMSO. Stock solutions were stored at −20˚C.
Conlon M., Poltorack C.D., Forcina G.C., Armenta D.A., Mallais M., Perez M.A., Wells A., Kahanu A., Magtanong L., Watts J.L., Pratt D.A, & Dixon S.J. (2021). A Compendium of Kinetic Modulatory Profiles Identifies Ferroptosis Regulators. Nature chemical biology, 17(6), 665-674.
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