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Eclipse ivi microscope

Manufactured by Nikon
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The ECLIPSE IVi microscope is a high-performance optical microscope designed for laboratory and research applications. It features advanced optics and illumination systems to provide clear, high-resolution imaging for a variety of specimens. The ECLIPSE IVi microscope is capable of various observation techniques, including brightfield, darkfield, and phase contrast.

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Lab products found in correlation

Alexa fluor 555, by Thermo Fisher Scientific (3 mentions) Digital sight ds u3, by Nikon (2 mentions) Digital sight ds u3 digital camera, by Nikon (2 mentions) Lsm710 confocal microscope, by Nikon (1 mentions) Pd minitrap sephadex g10 columns, by GE Healthcare (1 mentions) Periodic acid schiff, by Merck Group (1 mentions) Fitc labeled dextran molecules, by Merck Group (1 mentions)

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Eclipse microscope (191 citations)

5 protocols using eclipse ivi microscope

1

Quantifying Gut Microbiome Changes in Mosquitoes

Cited 2 times
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Mosquitoes were collected 5 day after dsRNA treatment or antibiotic treatment and surface sterilized with 70% ethanol twice and 0.9% NaCl twice. Midguts were dissected and homogenized in 0.9% NaCl. Homogenates were serially diluted and plated on LB agar plates. CFUs were counted 2 days after incubation at 28°C. Total DNA was extracted by the method of Holmes and Bonner as described [64 (link)]. Bacterial density was quantified by qPCR using universal 16S rRNA primers [28 (link)] (S1 Table). Ribosomal gene S7 was used as the internal reference. Significance was determined using the Student’s t-test.
The composition of the gut microbiota in dsRNA treated mosquitoes was analyzed by pyrosequencing that targeted the V3-V4 region of bacterial 16S rRNA [65 ]. 10 midguts of dsRNA treated mosquitoes were pooled for 1 biological replicate. DNA of 3 biological replicates of each treatment were prepared for further sequencing analysis (S1 Text).
For fluorescent in situ hybridization (FISH), abdomens of dsLD treated females 2 day post blood meal were fixed and sectioned as described [66 (link)]. Slides were hybridized with 10ng/μl universal 16S ribosomal RNA probe (5’-GCTGCCTCCCGTAGGAGT-3’) labeled with Alexa Fluor 555 (Life technology). Tissues were visualized using Nikon ECLIPSE IVi microscope connected to a Nikon DIGITAL SIGHT DS-U3 digital camera.
Song X., Wang M., Dong L., Zhu H, & Wang J. (2018). PGRP-LD mediates A. stephensi vector competency by regulating homeostasis of microbiota-induced peritrophic matrix synthesis. PLoS Pathogens, 14(2), e1006899.
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2

Mosquito Midgut Histology and Permeability

Cited 2 times
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Forty-eight hour post blood meal mosquito abdomens were fixed and sectioned as described above [66 (link)]. Samples were sectioned at 5 μm, stained with hematoxylin and eosin (H&E) (Huntz Enterprises Inc., China) and Periodic Acid Schiff (PAS) (Sigma-Aldrich, China) according to the manufacturer’s protocol. Slides were hard mounted using Canada balsam (ChemsWorth). Slides were viewed using bright field illumination on a Nikon ECLIPSE IVi microscope connected to a Nikon DIGITAL SIGHT DS-U3 digital camera. Four days post dsRNA treatment A. stephensi were fed with blood meal supplemented with 500 kDa FITC-labeled dextran molecules (2.5mg/ml blood)(Sigma) which were filtered using PD MiniTrap Sephadex G10 columns (GE Healthcare) as described [27 (link)]. Forty eight hours post-feeding, midguts were dissected and FITC signal observed using a Zeiss, LSM710 confocal microscope connected to a Nikon DIGITAL SIGHT DS-U3 digital camera. Expression of 4 PM genes was analyzed 24 hr and 48 hr post blood meal using primers targeting peritrophin1(ASTE010406), peritrophin14 (ASTE009456), 2 chitinases, herein named chitinaseA (ASTE005630) and chitinaseB (ASTE000328) (S1 Table).
Song X., Wang M., Dong L., Zhu H, & Wang J. (2018). PGRP-LD mediates A. stephensi vector competency by regulating homeostasis of microbiota-induced peritrophic matrix synthesis. PLoS Pathogens, 14(2), e1006899.
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3

Visualization of Asaia Bacteria in Mosquito Abdomens

Cited 1 time
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Abdomens of females fed on S and G sugar meals were fixed and sectioned as described previously (Attardo et al., 2008 (link)). Slides were hybridized with A. bogorensis-specific 16S rRNA probe, Asaia1 (5′-AGC ACC AGT TTC CCG ATG TTA T-3′) and Asaia2 (5′-GAA ATA CCC ATC TCT GGA TA-3′) labeled with Alexa Fluor® 555 (Thermo Fisher, China) (Favia et al., 2007 (link)). Tissues were visualized using a Nikon ECLIPSE IVi microscope connected to a Nikon DIGITAL SIGHT DS-U3 digital camera.
Wang M., An Y., Gao L., Dong S., Zhou X., Feng Y., Wang P., Dimopoulos G., Tang H, & Wang J. (2021). Glucose-mediated proliferation of a gut commensal bacterium promotes Plasmodium infection by increasing mosquito midgut pH. Cell reports, 35(3), 108992.
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4

Visualization of Asaia Bacteria in Mosquito Abdomens

Cited 1 time
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Abdomens of females fed on S and G sugar meals were fixed and sectioned as described previously (Attardo et al., 2008 (link)). Slides were hybridized with A. bogorensis-specific 16S rRNA probe, Asaia1 (5′-AGC ACC AGT TTC CCG ATG TTA T-3′) and Asaia2 (5′-GAA ATA CCC ATC TCT GGA TA-3′) labeled with Alexa Fluor® 555 (Thermo Fisher, China) (Favia et al., 2007 (link)). Tissues were visualized using a Nikon ECLIPSE IVi microscope connected to a Nikon DIGITAL SIGHT DS-U3 digital camera.
Wang M., An Y., Gao L., Dong S., Zhou X., Feng Y., Wang P., Dimopoulos G., Tang H, & Wang J. (2021). Glucose-mediated proliferation of a gut commensal bacterium promotes Plasmodium infection by increasing mosquito midgut pH. Cell reports, 35(3), 108992.
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5

TUNEL Staining for Apoptosis

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Fresh prepared slides of the abdomens prior to and 45 h post blood meal were used for TUNEL staining according to the manufacturers' instructions (Yeasen, Shanghai). Briefly, paraffin sections were dewaxed by xylenes and rehydrated with a graded series of ethanol. Tissue was permeabilized with 20 μg/ml proteinase K for 20 min at room temperature. After PBS rinse, slides were equilibrated with 1× Equilibration Buffer for 30 min at room temperature, then equilibration Buffer was removed and slides were incubated with TUNEL reaction mixture that contains Alexa Fluor 488-12-dUTP at 37 °C for 1 hr.
Apoptosis positive signal was acquired with 488 nm excitation using a Nikon ECLIPSE IVi microscope. Nuclei were stained with DAPI.
Feng Y., Peng Y., Han W., Song X., An Y., Tang H., & Wang J. (2021). Microbial tryptophan catabolism affects the vector competence ofAnopheles.
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