Disodium hydrogen phosphate

Manufactured by Beijing Chemical Works

Sourced in China

Disodium hydrogen phosphate is a chemical compound with the formula Na2HPO4. It is a colorless, crystalline solid that is commonly used as a buffering agent in laboratory applications. Disodium hydrogen phosphate helps maintain a desired pH range in various chemical and biological processes.

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Lab products found in correlation

4 protocols using disodium hydrogen phosphate

Simultaneous Quantification of Mycotoxins

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1 milligram standard mycotoxins powders of AFB₁, ZEN, DON, OTA, T-2 toxin and FB₁ (subsequently dissolved in Acetonitrile to give solutions of 1 mg·mL⁻¹ and stored at −20 °C), 1 mL isotope internal standard solutions of ¹³C₁₇-AFB₁, ¹³C₁₈-ZEN, ¹³C₁₅-DON, ¹³C₂₀-OTA, ¹²C₂₄-T-2 toxin and ¹³C₃₄-FB₁ prepared in Acetonitrile and stored at −20 °C (25 µg·mL⁻¹ for ¹³C₁₅-DON, ¹³C₁₈-ZEN, ¹²C₂₄-T-2 toxin and ¹³C₃₄-FB₁, 0.5 µg·mL⁻¹ for ¹³C₁₇-AFB₁ and 1 µg·mL⁻¹ for ¹³C₂₀-OTA), and Sephrose 4B gel (CNBr-activated) for IAC preparation were all purchased from Sigma-Aldrich (St. Louis, MO, USA). Acetonitrile, methanol, formic acid and ammonium formate (HPLC grade) were also from Sigma-Aldrich (St. Louis, MO, USA). Hydrochloric acid (HCl), sodium bicarbonate (NaHCO₃), sodium chloride (NaCl), tris, glacial acetic acid, potassium chloride, disodium hydrogen phosphate, potassium dihydrogen phosphate, sodium dihydrogen phosphate, and sodium azide (NaN₃) were of analytical grade, purchased from Beijing Chemical Reagent Company (Beijing, China). A Milli-Q water purification system was obtained from Millipore (Bedford, MA, USA), and the resistivity of water was 18.2 MΩ·cm at 25 °C.

Liu Y., Jin Y., Guo Q., Wang X., Luo S., Yang W., Li J, & Chen Y. (2022). Immunoaffinity Cleanup and Isotope Dilution-Based Liquid Chromatography Tandem Mass Spectrometry for the Determination of Six Major Mycotoxins in Feed and Feedstuff. Toxins, 14(9), 631.

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Fluoride Adsorption Optimization Protocol

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The chemicals used in this study were of analytical grade and did not require further purification. Nitric acid (HNO₃), sodium hydroxide (NaOH), sodium fluoride (NaF), sodium nitrate (NaNO₃), sodium chloride (NaCl), disodium hydrogen phosphate (Na₂HPO₄·12H₂O), sodium sulfate (Na₂SO₄) and sodium carbonate (Na₂CO₃) were purchased from Beijing Chemical Factory. Zirconium oxychloride octahydrate (ZrOCl₂·8H₂O) was purchased from Tianjin Guangfu Fine Chemical Research Institute. The fluoride concentrations and pH values of the aqueous solutions were measured using a PF-1-C type fluoride ion selective electrode and a pH meter, respectively, which were purchased from Shanghai Yue-Ci Electronic Technology Co., Ltd. The morphologies and elemental compositions of the adsorbents were observed using a scanning electron microscope and energy dispersive spectrometer (ZEISS), and the composition of the functional group of the adsorbent was observed using a Fourier transform infrared spectrometer (IRTracer-100). The fluoride stock solution was prepared by dissolving 2.21 g of NaF in 1000 mL deionized water. In addition, other solutions of fluoride ions with different concentrations were prepared by diluting the stock solution.

Zhang Y, & Huang K. (2019). Grape pomace as a biosorbent for fluoride removal from groundwater. RSC Advances, 9(14), 7767-7776.

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Isolation and Characterization of Chinese Fir Tannins

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Chinese fir (Cunninghamia lanceolate), sourcing from Fujian, China, was used as a raw material. Phosphoric acid, phenol, hydrochloric acid, hexamethyleneteramine, formaldehyde, sodium thiosulfate, disodium hydrogen phosphate, monopotassium phosphate, copper sulfate pentahydrate and zinc chloride were purchased from Beijing Chemical Works (Beijing, China). Soluble starch was purchased from Xilong Chemical Co. Ltd. (Shantou, China). Potassium iodide and iodine was purchase from Guangdong Guanghua Sc-tech Co. Ltd. (Shantou, China). Methylene blue was purchase from Tianjin Jinke Fine Chemicals Co. Ltd. (Tianjin, China) All the chemicals were of reagent grade and used without further purification.

Ma R., Qin X., Liu Z, & Fu Y. (2019). Adsorption Property, Kinetic and Equilibrium Studies of Activated Carbon Fiber Prepared from Liquefied Wood by Zncl2 Activation. Materials, 12(9), 1377.

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Rapid Immunoassay for Melamine Detection

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MEL and skimmed milk powder were purchased from Sigma-Aldrich (St. Louis, MO, USA). The consumptive materials in the ICA strip, namely, glass fiber and absorbent pad, were purchased from Jinbiao Biotech Co., Ltd (Shanghai, China). The nitrocellulose (NC) membrane filter was supplied by Millipore Corporation (Millipore, Bedford, MA). Ingredients for preparing phosphate buffer (PB) such as sucrose, Tween 20, PEG-20000, disodium hydrogen phosphate, sodium dihydrogen phosphate, and glycine were purchased from Beijing Chemical Reagent Company (Beijing, China). The anti-MEL monoclonal antibody (anti-MEL-mAb), coating antigen of MEL (MEL-BSA), goat anti-mouse IgG antibodies, and commercial MEL ELISA kit were purchased from Shandong Landu Bio-Science & Technology Co., Ltd (Shandong, China). The labeling probe was carboxylate-modified QB with an excitation wavelength of 365 nm and emission wavelength of 610 nm, which was supplied by Beijing Nanjing Biological Technology Co., Ltd (Beijing, China). Pure milk was collected from the local supermarket. All the other reagents were of the analytical grade and obtained from the National Pharmaceutical Group Chemical Reagent Co., Ltd (Shanghai, China).

Chen Q., Qie M., Peng X., Chen Y, & Wang Y. (2020). Immunochromatographic assay for melamine based on luminescent quantum dot beads as signaling probes. RSC Advances, 10(6), 3307-3313.

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