Anti sod2 mnsod antibody 2a1

Antibodies to total adenosine monophosphate kinase (AMPK, #2532S, 1:500), phosphorylated AMPK (pAMPK, #2532S, 1:500), Sirtuin 1 (SIRT1, #9475S, 1:250), Sirtuin 3 (SIRT3, #2627S, 1:500), total endothelial nitric oxide synthase (eNOS, #9572S, 1:500), and Ser1177 phosphorylated eNOS (#9571S, 1:500) were obtained from Cell Signaling (Danvers, MA, USA). The antibody cocktail to representative subunits of mitochondrial oxidative phosphorylation (Total OXPHOS Rodent WB Antibody Cocktail #ab110413, 1:1500) complexes I (subunit NDUF88), II (subunit SDHB), III (subunit UQCRC2), IV (MTCO1), and V (subunit ATP5A), PPARγ coactivator 1 alpha (PGC-1α, #ab54481, 1:500), and MnSOD antibody (Anti-SOD2/MnSOD antibody [2a1], #ab16956, 1:500) were obtained from Abcam (Cambridge, MA, USA). The catalase antibody was from Cell Signaling (#14097, 1:1000), CaMKIIα/β was from R&D Systems (#MAB7280, 1:1000). pCaMKII was from Cell Signaling (#12716, 1:1000). SOD was from Abcam (#ab0613498, 1:500). Secondary antibodies were IRDye 800RD goat anti-mouse #926-68070 at 1:10,000, IRDye 800RD goat anti-rabbit #926-68071 at 1:10,000 (LI-COR; Lincoln, NE, USA), and Starbright Blue 700 goat anti-mouse at 1:5000 #12004159, obtained from Bio-Rad Laboratories (Hercules, CA, USA).

Antibodies to total adenosine monophosphate kinase (AMPK, Cell Signaling #2532S, 1 : 500, mouse), phosphorylated AMPK (pAMPK, Cell Signaling #2532S, 1 : 500, rabbit), Sirtuin 3 (SIRT3, Cell Signaling #2627S, 1 : 500, rabbit), total endothelial nitric oxide synthase (eNOS, Cell Signaling #9572S, 1 : 500-1 : 250, mouse), Ser1177 phosphorylated eNOS (Cell Signaling #9571S, 1 : 500-1 : 250 rabbit), were obtained from Cell Signaling (MA, USA). Antibody cocktail to representative subunits of mitochondrial oxidative phosphorylation (Total OXPHOS Blue Native WB Antibody Cocktail Abcam #ab110412, 1 : 1000-1 : 500, mouse) complexes I (subunit NDUFA9), II (subunit SDHA), III (subunit UQCRC2), IV (subunit IV), and V (subunit ATP5A); PPARγ coactivator 1 alpha (PGC-1α, Abcam #ab54481, 1 : 500, rabbit); and MnSOD antibody (Anti-SOD2/MnSOD antibody [2a1], Abcam, #ab16956, 1 : 1000-1 : 500) were obtained from Abcam (Cambridge, MA). Secondary Fluorescent antibodies (IRDye 680RD goat antimouse, Li-COR, #926-68070 1 : 5,000, IRDye 680RD goat antirabbit, Li-COR, #926-68071 1 : 5,000, IRDye 800CW goat antimouse, Li-COR, #926-32210, 1 : 10,000, IRDy 800CW goat antirabbit, Li-COR, #926-32211, 1 : 10,000) for Western blot detection were purchased from Li-COR (NE, USA).