Ethanol (190 proof) was obtained from Decon Labs, Inc. Phosphate-buffered saline (PBS) was prepared in house by adding 2.7 mM KCl, 13.7 mM NaCl, 10 mM Na2HPO4, and 1.8 mM KH2PO4 to 18 MΩ Millipore water. Methacrylic anhydride, carbic anhydride, 5-Norbornene-2-carboxylic acid (endo/exo mixture), N-hydroxysuccinimide, N-(3-Dimethylaminopropyl)-N-ethylcarbodiimide (EDC), anhydrous DMSO, ninhydrin, and Sodium trimethylsilylpropanesulfonate (DSS) were obtained from Sigma. ninhydrin was dissolved in Ethanol to the stated concentration and used within two days. DSS was used as the internal standard (δ 0.0 ppm), and D2O (Cambridge Isotope Laboratories, Inc.) was used as the solvent in 1H-NMR experiments. DSS was dissolved in D2O to at 0.25 mg/mL to make the internal standard solution.
Sodium trimethylsilylpropanesulfonate dss
Manufactured by Merck Group
Sourced in Germany
Sodium trimethylsilylpropanesulfonate (DSS) is a chemical compound used as a reference standard in nuclear magnetic resonance (NMR) spectroscopy. It serves as an internal standard for calibrating the chemical shift scale in NMR experiments.
Automatically generated - may contain errors
Lab products found in correlation
Anhydrous dmso, by Merck Group (1 mentions)
Methacrylic anhydride, by Merck Group (1 mentions)
Ethanol, by Decon Labs (1 mentions)
N hydroxysuccinimide, by Merck Group (1 mentions)
Ninhydrin, by Merck Group (1 mentions)
5 norbornene 2 carboxylic acid, by Merck Group (1 mentions)
N 3 dimethylaminopropyl n ethylcarbodiimide edc, by Merck Group (1 mentions)
Carbic anhydride, by Merck Group (1 mentions)
Dextran sulfate sodium (dss), by Cambridge Isotopes (1 mentions)
Bovine serum albumin (bsa), by Merck Group (1 mentions)
Thionyl chloride, by Vekton (1 mentions)
1 2 dipalmitoyl sn glycero 3 phosphocholine dppc, by Avanti Polar Lipids (1 mentions)
Milli q water, by Thermo Fisher Scientific (1 mentions)
Sodium hydroxide (naoh), by Thermo Fisher Scientific (1 mentions)
Hydrochloric acid (hcl), by Thermo Fisher Scientific (1 mentions)
Lambda 35 spectrophotometer, by PerkinElmer (1 mentions)
4 protocols using sodium trimethylsilylpropanesulfonate dss
1
Synthesis and Characterization of Novel Polymers
2
Synthesis and Characterization of NPP
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The following commercially available reagents were used: fatty acids free BSA (Sigma Aldrich, Steinheim, Germany), NPA (Sigma Aldrich, Steinheim, Germany), sodium trimethylsilylpropanesulfonate (DSS, Sigma Aldrich, Steinheim, Germany), p-nitrophenol (Acros Organics, Geel, Belgium), propionic acid (Chemical Line, St. Petersburg, Russia), thionyl chloride (Vekton, St. Petersburg, Russia). Deuterated dimethyl sulfoxide (DMSO d6) and deuterated water were provided by the Centre for Magnetic Resonance of St. Petersburg State University. NPP was synthesised from p-nitrophenol and propionic acid using thionyl chloride in the Research Institute of Hygiene, Occupational Pathology and Human Ecology, Federal Medical Biological Agency; according to NMR analysis, NPP content was not less than 90%.
3
Synthetic Peptide Production and Characterization
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Egg-phosphatidylcholine (egg-PC), egg-phosphatidylglycerol (egg-PG), 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), and 1,2-dipalmitoyl-sn-glycero-3-phosphorylglycerol (DPPG) were obtained from Avanti Polar Lipids (Alabaster, AL, United States). Deuterated compounds (D38) DPC (dodecylphosphocholine) (98%), SDS (sodium dodecylsulfate) and D2O (99.9%) were from Cambridge Isotope Laboratories (United States). The percentages of deuteration are indicated in parenthesis. Non-deuterated DPC and SDS were from Avanti and Sigma, respectively. Sodium trimethylsilylpropanesulfonate (DSS) was from Sigma. Gemini surfactant [pentanediyl-1,5-bis(Hydroxyethylmethylhexadecylammonium bromide)] was synthesized by Dr. Razieh Amiri, University of Isphahan, Iran purified by three rounds of crystallization. Purity was >98%, as determined by TLC and NMR. The estimated CMC of this surfactant is about 2–3 μM at 25°C (Rosen and Tracy, 1998 ). Synthetic F. oxysporum WT α-pheromone (Trp-Cys-Thr-Trp-Arg-Gly-Gln-Pro-Cys-Trp; reduced and oxidized versions), a scrambled version (Scr) thereof (Trp-Arg-Trp-Pro-Cys-Cys-Trp-Gly-Gln-Thr) (Figure 1 ), and the dialanine substituted analogs D-Ala1,2 and D-Ala6,7 were obtained from GenScript (Piscataway, NJ, United States) or CASLO ApS (Denmark). All peptides were purified by HPLC to purities over 97.7% and lyophilized.
4
Spectroscopic Characterization of 4BBA
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Chromophore 4-benzoyl benzoic acid (4BBA) was purchased from Sigma Aldrich and used without further purification. Aqueous solutions of 4BBA were prepared using 18 MO Milli-Q water at a concentration of 100 mM. All the pH adjustments were carried out by adding either hydrochloric acid (1 N stock solution) or sodium hydroxide (1 N stock solution), both solutions from Fisher Chemical. Potentiometric titration for the measurement of pK a and pH measurements were carried out using an ACUMET AB15 pH meter. All UV-Vis spectral data were acquired using a PerkinElmer Lambda 35 spectrophotometer. Speciation and further pK a studies were carried out byfoot_0 H-NMR spectroscopy performed using a 500 Jeol ECA NMR spectrometer with solvent suppression in the acquisition parameters. The solution containing 10% D 2 O was used for instrument locking and 5 mM sodium trimethylsilylpropanesulfonate (DSS) purchased from Sigma Aldrich was used as the internal standard for peak locking.
In order to determine if the observed optical changes correspond to acid-base equilibria of the keto acid of 4BBA, or more complex speciation is responsible for the changes in the optical spectra of 4BBA, a combination of potentiometric titration and solvent-suppression 1 H-NMR spectra was carried out. The UV-Vis spectra for the 100 mM solution of 4BBA at pHs were acquired at pHs ranging from 1.0 to 12.0. Similarly,
In order to determine if the observed optical changes correspond to acid-base equilibria of the keto acid of 4BBA, or more complex speciation is responsible for the changes in the optical spectra of 4BBA, a combination of potentiometric titration and solvent-suppression 1 H-NMR spectra was carried out. The UV-Vis spectra for the 100 mM solution of 4BBA at pHs were acquired at pHs ranging from 1.0 to 12.0. Similarly,
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