After crizotinib and sunitinib treatment of HepG2 cells, the supernatant was collected and the biochemical parameters alanine aminotransferase (ALT), aspartic acid transferase (AST), alkaline phosphatase (ALP) and lactate dehydrogenase (LDH) were measured by the full-automatic clinical analyzer in the laboratory of the second Xiangya hospital (7600, HITACHI Ltd., Tokyo, Japan).
Liver samples of the mice were fixed in 10% phosphate-buffered formalin and embedded in paraffin. In brief, the liver tissue was embedded in paraffin, then deparaffinized with xylene, stained with hematoxylin and eosin, then dehydrated and sealed, and finally evaluated for damage under light microscopy.
Liver samples of the mice were fixed in 10% phosphate-buffered formalin and embedded in paraffin. In brief, the liver tissue was embedded in paraffin, then deparaffinized with xylene, stained with hematoxylin and eosin, then dehydrated and sealed, and finally evaluated for damage under light microscopy.