Transwell migration assays were performed using 24-well Transwell chamber plates (Corning, USA). PMVECs (1 × 105 cells/200 μl per well) were seeded in the upper compartment of the transwell chamber. The lower compartment was filled with 0.5 ml of the cell culture medium. After 12 h of incubation, the PMVECs that migrated through the polycarbonate membrane were fixed, stained, and counted under a microscope equipped with a digital imaging system (Nikon, Japan).
24 well transwell chamber plates
Manufactured by Corning
Sourced in United States
The 24-well Transwell chamber plates are a versatile laboratory tool used for various cell-based assays. They feature a polycarbonate membrane insert that creates a two-chamber system, allowing for the study of cell migration, permeability, and other cellular interactions. The plates are designed to facilitate efficient and reliable experiments while maintaining a consistent and controlled environment.
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Lab products found in correlation
2 protocols using 24 well transwell chamber plates
1
Transwell Migration Assay of PMVECs
2
Transwell Migration Assay for HLECs
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Transwell migration assays were performed with 24-well Transwell chamber plates (Corning, NY, USA). HLECs (8 × 104 cells/150 μL/well) were seeded in the upper compartment of the transwell chamber. The lower compartment was supplemented with 0.6 mL of the cell culture medium. After 12 h of incubation, the HLECs that had migrated through the polycarbonate membrane were fixed, stained, and enumerated. The images were analyzed with ImageJ software.
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