Antibodies against human ICAM-1 and human CD68 were obtained from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA). Antibodies against human phosphorylated and total NF-κB p65 were obtained from Cell Signaling Technology (Beverly, MA). Recombinant human CD18 protein and antibody against β-actin were obtained from Abcam (Cambridge, MA). ICAM-1 neutralizing antibody and antibody against phosphorylated YAP were obtained from Thermo Fisher Scientific (Waltham, MA). Medium 199 (M199) was obtained from Lonza (Walkersville, MD). Roswell Park Memorial Institute medium (RPMI 1640) and phosphate-buffered saline (PBS) were obtained from GIBCO Laboratories (Grand Island, NY). Recombinant human matrilin-2 (endotoxin-free), β2-integrin neutralizing antibody, YAP inhibitor verteporfin (VP), antibody against total YAP, and ELISA kit for IL-6 were obtained from R and D Systems (Minneapolis, MN). NF-κB inhibitor Bay 11-7082 was obtained from Sigma-Aldrich (St. Louis, MO).
Medium 199 m199
Manufactured by Lonza
Medium 199 (M199) is a cell culture medium formulated for the cultivation of various cell types. It is a balanced salt solution that provides essential nutrients, vitamins, and other components required for cell growth and maintenance. M199 is commonly used in a wide range of cell culture applications.
Automatically generated - may contain errors
Lab products found in correlation
Roswell park memorial institute medium rpmi 1640, by Thermo Fisher Scientific (2 mentions)
Antibody against β actin, by Abcam (1 mentions)
Nf κb inhibitor bay 11 7082, by Merck Group (1 mentions)
Elisa kit for il 6, by R&D Systems (1 mentions)
Phosphate buffered saline (pbs), by Thermo Fisher Scientific (1 mentions)
Ab137321, by Abcam (1 mentions)
Collagenase, by Merck Group (1 mentions)
Ab49900, by Abcam (1 mentions)
Myd88 d80f5, by Cell Signaling Technology (1 mentions)
Recombinant tnf α, by Merck Group (1 mentions)
Sc 7891, by Santa Cruz Biotechnology (1 mentions)
Pam3csk4, by InvivoGen (1 mentions)
2 protocols using medium 199 m199
1
Investigating ICAM-1 and NF-κB Signaling Pathways
2
Antibody Validation for Inflammatory Signaling
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Antibody against ICAM-1 (sc-7891) was obtained from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA). Antibodies against VCAM-1 (E1E8X) and MyD88 (D80F5) were obtained from Cell Signaling, Inc. (Beverly, MA). Antibody against TLR2 (NB100-56728) was obtained from Novus Biologicals, LLC (Centennial, CO). Antibodies against β-actin (ab49900) and GAPDH (ab9385) were obtained from Abcam (Cambridge, MA). Antibody against vimentin (ab137321) was obtained from Abcam (Cambridge, MA). Medium 199 (M199) was obtained from Lonza (Walkersville, MD). Roswell Park Memorial Institute medium (RPMI 1640) was obtained from GIBCO Laboratories (Grand Island, NY). Pam3CSK4 was obtained from InvivoGen (San Diego, CA). TLR2 inhibitor CU CPT 22 (4884) was obtained from Tocris Bioscience (Bristol, UK). Tumor necrosis factor-α (TNF-α) neutralizing antibody (MAB 610) was purchased from R&D System (Minneapolis, MN). Recombinant TNF-α, collagenase and other reagents were obtained from Sigma-Aldrich Chemical Co. (St Louis, MO).
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