Bzatp

Manufactured by Bio-Techne

Sourced in United States, United Kingdom

BzATP is a laboratory reagent produced by Bio-Techne. It functions as a selective agonist for the P2X7 receptor.

Automatically generated - may contain errors

Lab products found in correlation

Suramin, by Bio-Techne (1 mentions) Luminescent atp detection assay kit, by Abcam (1 mentions) Dynasore, by Merck Group (1 mentions) Cd11b fitc, by BD (1 mentions) Fluo 4 direct calcium assay kit, by Thermo Fisher Scientific (1 mentions) Primescript rt reagent kit, by Takara Bio (1 mentions) Ox atp, by Bio-Techne (1 mentions) Recombinant murine gm csf, by R&D Systems (1 mentions) Arl67156, by Bio-Techne (1 mentions) Psb 12062, by Merck Group (1 mentions) Mhc 2 pe, by BD (1 mentions) F4 80 pe, by BD (1 mentions) Cd11c apc, by BD (1 mentions) Sybr premix ex taq 2, by Takara Bio (1 mentions) Rnaiso plus, by Takara Bio (1 mentions) Interleukin 4 (il 4), by R&D Systems (1 mentions) Bca assay, by Thermo Fisher Scientific (1 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions) A438079, by Abcam (1 mentions) Adenosine triphosphate (atp), by Merck Group (1 mentions)

Spelling variants of this product

BzATP triethylammonium salt (2 citations)

5 protocols using bzatp

Murine Dendritic Cell Characterization

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Female C57BL/6 mice purchased from Ji'nan Pengyue Laboratory Animal Breeding Co., Ltd. (China) were housed and maintained in the animal facilities of Weifang Medical University. Recombinant murine GM-CSF and IL-4 were purchased from R&D Systems (USA). A Fluo-4 Direct™ calcium assay kit was purchased from ThermoFisher Scientific (USA). ARL-67156, Bz-ATP, OX-ATP, and suramin were purchased from Tocris Bioscience (USA). A-438079, a competitive P2X7 receptor antagonist, was purchased from Santa Cruz (USA). Luminescent ATP Detection Assay kit was purchased from Abcam (USA). The endocytosis inhibitors MDC, PitS2, Dynasore, and P2X4-specific antagonist PSB-12062 were purchased from Sigma-Aldrich (USA). RNAiso Plus, SYBR Premix Ex Taq II, and the PrimeScript^TM RT reagent Kit were obtained from Takara (China). CD11c-APC (#550261), CD11b-FITC (#553310), MHC II-PE (#558593), and F4/80-PE (#565410) and isotype control antibodies were purchased from BD Bioscience (USA). CD39-PE (#12-0391-82) was purchased from Thermo Fisher (USA). All experiments involving animals were performed in accordance with the Chinese National Laboratory Animal-Guideline for Ethical Review of Animal Welfare and approved by the Institutional Animal Care and Use Committee of Weifang Medical University (NO. 010/2017).

Zhao R., Qiao J., Zhang X., Zhao Y., Meng X., Sun D, & Peng X. (2019). Toll-Like Receptor-Mediated Activation of CD39 Internalization in BMDCs Leads to Extracellular ATP Accumulation and Facilitates P2X7 Receptor Activation. Frontiers in Immunology, 10, 2524.

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BzATP-Induced IL-1β Secretion in BV2 Microglia

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BV2 microglia-like cells were maintained in DMEM (Gibco) containing 5% FBS and 1% penicillin-streptomycin at 37°C with 5% CO₂. BV2 cells were harvested from a 100-mm dish using a cell scraper, collected in 500 μl, and equally aliquoted into Eppendorf tubes before treatment. Cells were stimulated with 300 μM BzATP (Tocris) in ECS for 30 min at 37°C. In a subset of experiments, cells were treated with 10 μM ¹⁰panx or ^scrpanx (New England Peptide) in ECS for 30 min at 37°C before BzATP stimulation. As a control, ECS alone was used in equivalent volumes to BzATP. Enzyme-linked immunosorbent assay (ELISA) kits for mouse and rat IL-1β (R&D Systems) were used for quantification of IL-1β in cell culture supernatants or spinal cord homogenates as per the manufacturer’s instruction. The concentration of IL-1β was normalized to protein levels (BCA Assay, Life Technologies) for spinal cord homogenates.

Mousseau M., Burma N.E., Lee K.Y., Leduc-Pessah H., Kwok C.H., Reid A.R., O’Brien M., Sagalajev B., Stratton J.A., Patrick N., Stemkowski P.L., Biernaskie J., Zamponi G.W., Salo P., McDougall J.J., Prescott S.A., Matyas J.R, & Trang T. (2018). Microglial pannexin-1 channel activation is a spinal determinant of joint pain. Science Advances, 4(8), eaas9846.

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ATP-Induced Thymocyte Apoptosis and Pore Formation

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Thymocytes were cultured at 4×10⁶ cells/mL with/without 1mM ATP (Sigma) or 100μM BzATP (Tocris) in culture medium (RPMI 1640, 10% FCS, penicillin/streptomycin/glutamine). For experiments using A438079 (Abcam), cells were pre-treated with 10μM or 100μM A438079 for 1 hour before addition of ATP or BzATP. After 15 minutes of stimulation, cells were harvested, washed, stained for Annexin V binding (Apoptosis Detection kit, BD Biosciences). To measure pore formation, 2μM YO-PRO-1 was added to the culture for the last 5 minutes before harvesting, washing, and staining.

Philips R.L., McCue S.A., Rajcula M.J, & Shapiro V.S. (2019). HDAC3 protects DP thymocytes from P2X7 receptor-induced cell death. Journal of immunology (Baltimore, Md. : 1950), 202(4), 1033-1038.

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Electrophysiological Recordings of P2X7 Receptor-Mediated Currents

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Brain slices were prepared as indicated in the Supplementary Material. Drugs were applied onto the cell under investigation by means of a glass-pipette (3–5 μm tip diameter) connected to a pneumatic drug ejection system (PDES-02DX, NPI Elect ronic GmbH, Germany). Ligand-gated currents were evoked by the P2X7 receptor agonist, BzATP (2′(3′)-O-(4-Benzoylben zoyl) adenosine 5′-triphosphate 100 μM; 3 sec; Sigma Aldrich, Spain). When used, 300 μM PL (Sigma Aldrich, Spain), 300 μM PLP or 10 μM A438079 (3-[[5-(2, 3-dichlorophenyl)-1H-tetrazol-1- yl] methyl]pyridine hydrochloride) (Tocris Biosc iences, Bristol, United Kingdom) were applied from a separate glass pipette 2 min before and during BzATP administration. Stock solutions of drugs were make freshly every day in the extracel lular saline, and the application pipettes were filled with the druǵs solutions a few minutes before starting the experiments.

Sebastián-Serrano Á., Engel T., de Diego-García L., Olivos-Oré L.A., Arribas-Blázquez M., Martínez-Frailes C., Pérez-Díaz C., Millán J.L., Artalejo A.R., Miras-Portugal M.T., Henshall D.C, & Díaz-Hernández M. (2016). Neurodevelopmental alterations and seizures developed by mouse model of infantile hypophosphatasia are associated with purinergic signalling deregulation. Human Molecular Genetics, 25(19), 4143-4156.

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ATP and bzATP Stimulation of MDMi

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MDMi on day 11 of differentiation are treated with 1 mM ATP or 500 uM bzATP for 3 hours at 37°C. As both are resuspended in DMSO, DMSO is added to controls. ATP was purchased from Theromfisher (#R0441) and bzATP was purchased from Tocris Bioscience (#3312).

Heavener K., Kabra K., Yidenk M, & Bradshaw E. (2024). IL-1RA Disrupts ATP Activation of P2RX7 in Human Monocyte-Derived Microglia-like Cells. bioRxiv.

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