CX3CR1-GFP homozygous mice (Jackson Laboratory), 6–8 wk of age, were used as BMM donors. Briefly, the femur was removed, and bone marrow cells were dissociated into single-cell suspensions and were cultured for 5 d supplemented with 1,000 U/ml of macrophage colony-stimulating factor.
Cx3cr1 gfp homozygous mice
Manufactured by Jackson ImmunoResearch
Sourced in United States
CX3CR1-GFP homozygous mice express green fluorescent protein (GFP) under the control of the CX3CR1 gene promoter. CX3CR1 is a chemokine receptor involved in the recruitment and migration of specific immune cells.
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5 protocols using cx3cr1 gfp homozygous mice
1
Isolation and Culture of Bone Marrow-Derived Macrophages
2
CX3CR1-GFP Mice Husbandry and Care
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C57BL/6 mice (male, 6–8 weeks) were purchased from Charles River Laboratories, Inc., CX3CR1-GFP homozygous mice were obtained from the Jackson Laboratory (Bar Harbor, ME, United States). These mice have a targeted deletion of CX3CR1 that is replaced by a GFP reporter insertion. All animals were housed under conditions of constant temperature and humidity on a 12-h light/12-h dark cycle, with lights on at 7:00 AM. Food and water were available ad libitum. All animal procedures were performed according to the protocols approved by the Institutional Animal Care and Use Committee at the University of Nebraska Medical Center.
3
Microglial Response in CX3CR1-GFP Mice
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C57BL/6N mice (male, 6–8 weeks) were purchased from Charles River Laboratories, Inc. (Wilmington, MA). CX3CR1-GFP homozygous mice were obtained from the Jackson Laboratory (Bar Harbour, Maine) and function by targeted deletion of CX3CR1 and GFP reporter insertion. These mice are known to exhibit a rapid microglial response injury as shown by Davalos et al.1 (link)41 (link) in response to ATP. All the animals were housed under conditions of constant temperature and humidity on a 12-h light, 12-h dark cycle, with lights on at 0700, h. Food and water were available ad libitum. Animals were deeply anaesthetized by overdose of isoflurane followed by pneumothorax before perfusion. All animal procedures were performed according to the protocols approved by the Institutional Animal Care and Use Committee of the University of Nebraska Medical Center.
4
CX3CR1-GFP Mice for Neurological Research
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C57BL/6 mice (male, 6–8 wk) were purchased from Charles River Laboratories, Inc. CX3CR1-GFP homozygous mice were obtained from the Jackson Laboratory and have a targeted deletion of CX3CR1 that is replaced by a GFP reporter insertion. All animals were housed under conditions of constant temperature and humidity on a 12-h light/12-h dark cycle, with lights on at 7:00 a.m. Food and water were available ad libitum. All animal procedures were performed according to the protocols approved by the Institutional Animal Care and Use Committee at the University of Nebraska Medical Center.
5
Isolation of Bone Marrow Macrophages
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CX3CR1-GFP homozygous mice (Jackson Laboratory), 6–8 weeks of age, were used as BMM donors as previously described (Niu et al., 2019 (link)). Briefly, the femur of CX3CR1-GFP mice was removed, and bone marrow cells were dissociated into single-cell suspensions that were supplemented with 1,000 U/ml of macrophage colony-stimulating factor and cultured for 5 days.
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