Mg132 z leu leu leu cho

NH₄Cl, 2,2′-dipyridyl, CoCl₂, Dulbecco’s modified Eagle’s medium (DMEM), ScreenFect™ A, an anti-DYKDDDDK (FLAG) antibody, and an anti-Myc tag monoclonal antibody were purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan). Penicillin–streptomycin solution, fetal bovine serum (FBS), geneticin (G418), CHX, bafilomycin A1, and 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) were obtained from Sigma Chemical Co. (St. Louis, MO, USA). MG132 (Z-Leu-Leu-Leu-CHO) was purchased from the Peptide Institute (Osaka, Japan). Isogen was from Nippon Gene (Toyama, Japan), Revert Aid™ M-MuLV Reverse Transcriptase from MBI Fermentas (Vilnius, Lithuania), Go Taq polymerase from Promega (Madison, WI), and KOD Fx Neo from Toyobo (Tokyo, Japan). The DNA Ligation Kit was obtained from Takara Bio Inc. (Shiga, Japan). 4′,6-Diamidino-2-phenylindole (DAPI) was purchased from Dojindo (Kumamoto, Japan). Anti-Parkin and anti-Hsc70 antibodies were from ProteinTech (Rosemont, IL, USA). An anti-ubiquitin antibody (clone FK2) was from StressMarq Bioscience. Alexa Fluor® 594-conjugated goat anti-mouse IgG and Alexa Fluor® 647-conjugated goat anti-rabbit IgG were obtained from Abcam (Carlsbad, CA, USA).