Dfc300f digital camera

Semithin analysis of sciatic nerves and ultrastructural analysis of sciatic and optic nerves were performed as described previously (Wrabetz et al, 2000).
To perform morphometric analysis, digitalized images of fiber cross sections were obtained from corresponding levels of the quadriceps or sciatic nerves with a 100× objective and Leica DFC300F digital camera (Milan, Italy). Five images per animal were analyzed using the Leica QWin software (Leica Microsystem) and the g‐ratio calculated as the ratio between the mean diameter of an axon (without myelin) and the mean diameter of the same axon including the myelin sheath.
For morphometric analysis on ultrastructural sections, 20 images per animal were taken at 4,000× (LEO 912AB Transmission Electron Microscope, Milan, Italy) and the g‐ratio values determined by measuring axon and fiber diameters.

Three to five animals per genotype for each time point were analysed. Semithin analysis of quadriceps and sciatic nerves and ultrastructural analysis of optic nerves and spinal cords were performed as described previously [62 (link)].
To perform morphometric analysis, digitalized images of fiber cross sections were obtained from corresponding levels of the quadriceps or sciatic nerves with a 100x objective and Leica DFC300F digital camera (Milan, Italy). Five images per animal were analysed with the Leica QWin software (Leica Microsystem) and the g-ratio calculated as the ratio between the mean diameter of an axon (without myelin) and the mean diameter of the same axon including the myelin sheath.
For morphometric analysis on ultrastructural sections, 20 images per animal were taken at 4000x (LEO 912AB Transmission Electron Microscope, Milan, Italy) and the g-ratio values determined by measuring axon and fiber diameters.
Internodal lengths were measured as described using Openlab (PerkinElmer) [19 (link)], and 100 internodes of two quadriceps nerves were evaluated for each animal (n = 3).