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2 protocols using chicken anti gfp ab13970 antibody

1

Immunolabeling of Apoptosis and Cytoskeleton

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Chicken anti-GFP (ab13970) antibody was purchased from Abcam. Rat anti-E-cadherin (131900) antibody was from Life Technologies. Alexa-Fluor-647-conjugated rabbit anti-cleaved caspase-3 (9602S) antibody was purchased from Cell Signaling Technology. Alexa-Fluor-568-conjugated phalloidin from Life Technologies was used at 1.0 U/mL. Alexa-488-conjugated secondary antibody was from Abcam, and Alexa-Fluor-568- and 647-conjugated secondary antibodies were from Life Technologies. Hoechst 33342 (Life Technologies) was used at a dilution of 1:5,000. TMRM was obtained from Molecular Probes. Palmitic acid, stearic acid, linoleic acid, α-linolenic acid, sphingomyelin, cholesterol, and Nile Red were from Wako Pure Chemicals Industries. Acetylsalicylic acid (aspirin) was from Sigma-Aldrich. Trimetazidine (Abcam) was used at 10 μM.
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2

Immunolabeling of Apoptosis and Cytoskeleton

Check if the same lab product or an alternative is used in the 5 most similar protocols
Chicken anti-GFP (ab13970) antibody was purchased from Abcam. Rat anti-E-cadherin (131900) antibody was from Life Technologies. Alexa-Fluor-647-conjugated rabbit anti-cleaved caspase-3 (9602S) antibody was purchased from Cell Signaling Technology. Alexa-Fluor-568-conjugated phalloidin from Life Technologies was used at 1.0 U/mL. Alexa-488-conjugated secondary antibody was from Abcam, and Alexa-Fluor-568- and 647-conjugated secondary antibodies were from Life Technologies. Hoechst 33342 (Life Technologies) was used at a dilution of 1:5,000. TMRM was obtained from Molecular Probes. Palmitic acid, stearic acid, linoleic acid, α-linolenic acid, sphingomyelin, cholesterol, and Nile Red were from Wako Pure Chemicals Industries. Acetylsalicylic acid (aspirin) was from Sigma-Aldrich. Trimetazidine (Abcam) was used at 10 μM.
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