Insulin i2643

Manufactured by Merck Group

Insulin (I2643) is a laboratory reagent produced by the Merck Group. It is a naturally occurring hormone that regulates blood sugar levels in the body. This product is intended for use in research and laboratory settings.

Automatically generated - may contain errors

Lab products found in correlation

H3k27ac ab4729, by Abcam (1 mentions) Flag f7425, by Merck Group (1 mentions) U 13c glucose clm 1396 1, by Cambridge Isotopes (1 mentions) Mk2206 s1078, by Selleck Chemicals (1 mentions) Glut1 ab652, by Abcam (1 mentions) Scc064, by Merck Group (1 mentions)

Spelling variants of this product

Insulin (4936 citations) I2643 (16 citations) Recombinant human insulin (I2643) (2 citations)

2 protocols using insulin i2643

Metabolic Profiling of Cellular Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols

GLUT-1 (ab652) and H3K27Ac (ab4729) were purchased from Abcam. HK (A0994) and horseradish peroxidase (HRP)-goat anti-Rabbit were purchased from ABclonal. HRP-conjugate anti-mouse (w402b) was purchased from Promega. H2BK (07-373), H4K (06-866), and H3K (06-599) were purchased from Millipore. Flag (F7425) was purchased from Sigma-Aldrich. All other antibodies including Rictor (2140), PRAS40 (2691), AKT (9272), GSK3b (9315), ACC (3676), ACLY (4332), FASN (3180), PPARg (2443), ACSS2 (3658), S6 (2217), S473-AKT (4058), T308-AKT (4056), S455-ACLY (4331), T246-pPRAS40 (2997), HA-tag (2367), S9-GSK3b (9315), S235/236-S6 (4858), H3 (9715), H3K9Ac (9649), H3K14Ac (7627), and a-Tubulin (2125) were purchased from Cell Signaling Technologies. 4-OHT was obtained from Toronto Research Chemicals. D-[U-¹⁴C]-glucose (NEC042V250UC) was purchased from Perkin Elmer. MK2206 (S1078) was purchased from Selleck Chemicals. T3 (T2877), dexamethasone (D1756), IBMX (I5879), and insulin (I2643) were from Sigma-Aldrich. [U-¹³C] glucose (CLM-1396-1) and [1,2-¹³C] acetate (CLM-440-PK) were purchased from Cambridge Isotope Laboratories, Inc.

Martinez Calejman C., Trefely S., Entwisle S.W., Luciano A., Jung S.M., Hsiao W., Torres A., Hung C.M., Li H., Snyder N.W., Villén J., Wellen K.E, & Guertin D.A. (2020). mTORC2-AKT signaling to ATP-citrate lyase drives brown adipogenesis and de novo lipogenesis. Nature Communications, 11, 575.

+ Open protocol

+ Expand

Establishing insulin resistance in LX-2 cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

LX‐2 cells (SCC064; Sigma‐Aldrich) were cultured in minimum essential medium (12360038; Gibco) with 10% fetal bovine serum (FBS, 16140071; Gibco) at 37°C with 5% CO₂. The IR cell model was established as follows¹⁵: LX‐2 cells were processed by serum‐starving for 12 h (h), followed by treatment with 18 mM glucosamine (PHR1199; Sigma‐Aldrich) for 18 h in the serum‐free medium. Finally, cells were stimulated with 100 nM insulin (I2643; Sigma‐Aldrich) for 30 min (min).¹⁶

Zhong G., Yang Q., Wang Y., Liang Y., Wang X, & Zhao D. (2023). Long noncoding RNA X‐inactive specific transcript (lncRNA XIST) inhibits hepatic insulin resistance by competitively binding microRNA‐182‐5p. Immunity, Inflammation and Disease, 11(11), e969.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!