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Hemavet analyzer

Manufactured by Drew Scientific
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The Hemavet analyzer is a compact and automated hematology analyzer designed for use in clinical laboratories. It is capable of performing a complete blood count (CBC) analysis, including the enumeration of red blood cells, white blood cells, and platelets. The Hemavet analyzer provides accurate and reliable results, allowing healthcare professionals to make informed decisions regarding patient care.

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Lab products found in correlation

H9262, by Merck Group (2 mentions) Gdc 0941, by Genentech (2 mentions) Microtainer, by BD (1 mentions) Cpg odn 1826, by InvivoGen (1 mentions) Female c57bl 6 mice, by Jackson ImmunoResearch (1 mentions) Anti ctla 4 clone 9h10, by BioXCell (1 mentions) Legendplex software, by BioLegend (1 mentions) Cytoflex flow cytometer, by BioLegend (1 mentions) Legendplex mouse inflammation panel 13 plex, by BioLegend (1 mentions) Aspartate aminotransferase activity assay kit, by Merck Group (1 mentions) Annexin 5 kit, by Thermo Fisher Scientific (1 mentions) Buffered formalin, by Thermo Fisher Scientific (1 mentions) Leica dm il led microscope, by Leica Microsystems (1 mentions) Las v41, by Leica Microsystems (1 mentions) Mcc950, by Cayman Chemical (1 mentions) Ibrutinib, by Selleck Chemicals (1 mentions) Thrombin, by Roche (1 mentions) Adenosine 5 diphosphate, by Merck Group (1 mentions) Facscalibur cytometer, by BD (1 mentions) Heparinized tube, by BD (1 mentions)

15 protocols using hemavet analyzer

1

Blood Platelet Count via Hemavet Analyzer

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A sample of blood was placed in EDTA-coated vials (BD Microtainer) and Hemavet analyzer (Drew Scientific Inc, Dallas, TX) was used to generate blood platelet counts.
Wu H., Yang J., Su E.M., Li L., Zhao C., Yang X., Gao Z., Pan M., Sun P., Sun W., Jiang Y, & Su X. (2014). Lipoxin A4 and Platelet Activating Factor Are Involved in E. coli or LPS-Induced Lung Inflammation in CFTR-Deficient Mice. PLoS ONE, 9(3), e93003.
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2

Induction and Characterization of Systemic Lupus Erythematosus in Mice

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sHLH was induced as previously described.16 (link) In brief, C57BL/6 female mice were injected intraperitoneally on days 0, 2, 4, 7, and 9 with 50 μg of CpG-ODN 1826 (Invivogen). At each indicated time point, mice were euthanized by CO2 inhalation. Body and spleen weights were recorded. Peripheral blood was collected in Microtainer BD tubes containing EDTA anticoagulant (Becton Dickinson). A complete blood count was performed on a Hemavet analyzer (Hematology Analyzer [950FS], Drew Scientific).
Buatois V., Chatel L., Cons L., Lory S., Richard F., Guilhot F., Johnson Z., Bracaglia C., De Benedetti F., de Min C., Kosco-Vilbois M.H, & Ferlin W.G. (2016). Use of a mouse model to identify a blood biomarker for IFNγ activity in pediatric secondary hemophagocytic lymphohistiocytosis. Translational Research, 180, 37-52.e2.
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3

Comparative Biodistribution of Micro-Particles in Mice

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Female C57BL/6 mice (Jackson) between 6-10 weeks of age were injected via tail vein with either 2 μm or 0.5 μm particles, dosed at equivalent mass. Mice received 2x108 2 μm particles or 1.28×1010 0.5 μm in 200 μL injection volume, corresponding to ∼0.6mg/mouse, ∼30mg/kg. These dosages were chosen as they are within a particle mass range typically used in drug delivery preclinical mouse evaluations21 (link),47 (link)-49 (link) and are appropriate to translate to human doses.50 (link) Blood was collected at various time points either via cardiac puncture or saphenous vein. Samples collected via cardiac puncture were collected in heparin as an anti-coagulant and immediately stained with anti-Ly6G-APC, anti-CD45-PE/Cy7, and anti-CD11b-APC/Cy7 on ice. After 30 minutes of staining, blood was treated with 1× RBC Lyse/Fix (eBioscience) following recommended protocol and analyzed via flow cytometry. Flow cytometry data was collected on an Attune N×T Focusing flow cytometer (Life Technologies) and analyzed using FlowJo software (Tree Star). Samples collected via saphenous vein for NΦ concentrations were collected in EDTA coated tubes and analyzed using a Hemavet Analyzer (Drew Scientific) Complete Blood Counter (CBC).
Fromen C.A., Kelley W.J., Fish M.B., Adili R., Noble J., Hoenerhoff M.J., Holinstat M, & Eniola-Adefeso O. (2017). Neutrophil-Particle Interactions in Blood Circulation Drive Particle Clearance and Alter Neutrophil Responses in Acute Inflammation. ACS nano, 11(11), 10797-10807.
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4

Neutrophil Depletion and LPS-Induced Inflammation

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For select groups of mice, neutrophils were depleted before LPS installation and intravital microscopy. Each mouse’s baseline complete blood count was measured using saphenous vein blood draws collected into EDTA-coated tubes and measured using a Hemavet Analyzer (Drew Scientific). Then, each mouse was given an IP injection of 500 µg anti-Ly6G depletion antibody (BioLegend #127649). After one day, the complete blood count for each mouse was again measured. Mice were utilized if neutrophil counts were depleted at or below the minimum normal range. Depletion mice received an additional 200 µg anti-Ly6G, administered via retro-orbital injection at the same time as labeling antibodies. A subset of these mice also received a prevention UT treatment of 2 µm PS particles. Like the other experimental groups, systemic inflammation was induced via IP injection of LPS 3 h before intravital microscopy. Neutrophil depletion was again confirmed by measuring the complete blood count of mice at the time of euthanasia via cardiac puncture blood draw.
Banka A.L., Guevara M.V., Brannon E.R., Nguyen N.Q., Song S., Cady G., Pinsky D.J., Uhrich K.E., Adili R., Holinstat M, & Eniola-Adefeso O. (2023). Cargo-free particles divert neutrophil-platelet aggregates to reduce thromboinflammation. Nature Communications, 14, 2462.
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5

Evaluation of Systemic Inflammation in Mice

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Naïve BALB/c mice were administered CpG—siRNA and Nucl—siRNA conjugates as described in the vaccination regimen in transplantable tumor models. Blood was collected from mice 2 days after last Nucl-siRNAs administration dose. Complete blood count (CBC) analysis was performed using HemaVet analyzer (Drew Scientific). Liver enzymes AST and ALT were quantified in serum by using a colorimetric Aspartate Aminotransferase Activity Assay Kit (Sigma) or Alanine Aminotransferase Activity Assay Kit (Cayman Chemical), respectively, according to the manufacturer’s protocol. Cytokines were quantified in serum by LEGENDplex Mouse Inflammation panel (13-plex; BioLegend) according to the manufacturer’s recommendations. Cytokine concentration in serum was analyzed using a CytoFLEX flow cytometer and LEGENDplex software (BioLegend). As a positive control of systemic inflammation, mice were injected with 200 μg of anti-CTLA4 clone 9H10 (BioXcell) as described previously (30 (link), 31 ). Lungs, livers, and small intestines were fixed in 10% formalin, and embedded in paraffin, and sectioned. Seven-micron—thick sections were stained with H&E to assess inflammation.
Garrido G., Schrand B., Levay A., Rabasa A., Ferrantella A., Da Silva D.M., D’Eramo F., Marijt K.A., Zhang Z., Kwon D., Kortylewski M., Kast W.M., Dudeja V., van Hall T, & Gilboa E. (2020). Vaccination against Nonmutated Neoantigens Induced in Recurrent and Future Tumors. Cancer immunology research, 8(7), 856-868.
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6

Monitoring Aflatoxin B1 and Cytokines

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Blood was collected at specific times prior to, during, and following the hemoperfusion session. Hematological analysis was conducted with a Hemavet analyzer (Drew Scientific). Circulating AFB1 and cytokines were measured by ELISA (Helica Biosystems, Santa Ana, CA) and Multi-plex immunoassay (Bio-Plex Th1/Th2 Rat Cytokine Assay Kit; Bio-Rad Laboratories), respectively.
Ruggeberg K.G., O’Sullivan P., Kovacs T.J., Dawson K., Capponi V.J., Chan P.P., Golobish T.D, & Gruda M.C. (2020). Hemoadsorption Improves Survival of Rats Exposed to an Acutely Lethal Dose of Aflatoxin B1. Scientific Reports, 10, 799.
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7

Blood Cell Analysis Workflow

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Blood was collected using heparinized capillary tubes and analyzed on a Hemavet Analyzer (Drew Scientific). For Flow cytometry analysis, spleen cells or peripheral blood were made into a single cell suspension, washed with HBSS+2%FBS and stained with the appropriate antibodies. Cells were analyzed in HBSS+2% FBS and 0.4ug/ml propidium iodide. Flow cytometry analysis was performed on Cyan (Beckman Coulter) and LSRII (BD) flow cytometers. Flow cytometry data were analyzed using FlowJo software (TreeStar Inc.). For apoptosis assays, cells were prepared as described in the Annexin-V kit (Life Technologies).
Souroullas G.P., Fedoriw Y., Staudt L.M, & Sharpless N.E. (2017). Lkb1 deletion in murine B lymphocytes promotes cell death and cancer. Experimental hematology, 51, 63-70.e1.
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8

Murine MDS/MPN Combination Therapy

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GDC-0941 (Genentech) was administered at the maximally tolerated dose (MTD) of 125 mg/kg/day or 0.5% hydroxypropylmethylcellulose (Sigma-Aldrich H9262)/0.2% Tween-80 vehicle by gavage once daily. MK-2206 (Proactive) was given at 240 mg/kg in Captisol vehicle (Ligand Pharmaceuticals) by gavage every Monday, Wednesday and Friday. Treatment of KrasD12 mice began at 8–10 weeks of age, and treatment of Nf1Δ/− mice began at 6 months of age; in both cases, MDS/MPN was well established prior to therapy. Mice were removed from trial if moribund or severely anemic (Hb < 6 g/dL). Blood counts were measured by Hemavet analyzer (Drew Scientific). Mice were randomly assigned to treatment groups without respect to sex; all experiments were unblinded.
Akutagawa J., Huang T.Q., Epstein I., Chang T., Quirindongo-Crespo M., Cottonham C.L., Dail M., Slusher B.S., Friedman L.S., Sampath D, & Braun B.S. (2016). Targeting the PI3K/Akt pathway in murine MDS/MPN driven by hyperactive Ras. Leukemia, 30(6), 1335-1343.
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9

Murine MDS/MPN Combination Therapy

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GDC-0941 (Genentech) was administered at the maximally tolerated dose (MTD) of 125 mg/kg/day or 0.5% hydroxypropylmethylcellulose (Sigma-Aldrich H9262)/0.2% Tween-80 vehicle by gavage once daily. MK-2206 (Proactive) was given at 240 mg/kg in Captisol vehicle (Ligand Pharmaceuticals) by gavage every Monday, Wednesday and Friday. Treatment of KrasD12 mice began at 8–10 weeks of age, and treatment of Nf1Δ/− mice began at 6 months of age; in both cases, MDS/MPN was well established prior to therapy. Mice were removed from trial if moribund or severely anemic (Hb < 6 g/dL). Blood counts were measured by Hemavet analyzer (Drew Scientific). Mice were randomly assigned to treatment groups without respect to sex; all experiments were unblinded.
Akutagawa J., Huang T.Q., Epstein I., Chang T., Quirindongo-Crespo M., Cottonham C.L., Dail M., Slusher B.S., Friedman L.S., Sampath D, & Braun B.S. (2016). Targeting the PI3K/Akt pathway in murine MDS/MPN driven by hyperactive Ras. Leukemia, 30(6), 1335-1343.
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10

Spleen Iron Content Analysis

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Whole spleens were collected from indicated mice and preserved in 10% buffered formalin (Fisher Scientific). Paraffin embedding, tissue sectioning, and Perls’ Prussian blue iron staining were performed by the Tissue Resources Core Facility of the Case Comprehensive Cancer Center (Cleveland, OH). Microscopy images were acquired using a Leica DM IL LED microscope and LAS V4.1 software (Leica Microsystems). Hematological differentials were performed using a HEMAVET analyzer (Drew Scientific).
Ryan S.O., Abbott D.W, & Cobb B.A. (2014). Myeloid Glycosylation Defects Lead to Spontaneous a CVID-Like Condition with Associated Hemolytic Anemia and Anti-Lymphocyte Autoimmunity. Journal of immunology (Baltimore, Md. : 1950), 192(12), 5561-5570.
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