Avii spectrometer

Un-polymerized soybean oil and the synthesized SBC were characterized by using a Nicolet-560 FTIR spectrometer with a resolution setting of 4 cm^-1. The scanning range was altered from 400 to 4,000 cm^-1. H¹-NMR (400 MHz) spectrum of both soybean oil and the SBC was recorded on a Bruker AV-II spectrometer, using tetramethylsilane (TMS) as an internal standard in DMSO-d₆ and CDCl₃ as the solvent. Gel permeation chromatography (GPC) test of the synthesized SBC was performed by using an HLC-8320 GPC (Japan) at 25°C. Tetrahydrofuran and polystyrene with a narrow molecular weight distribution were used as the eluent and the reference, respectively. The flow speed of the solution was 1 mL/min. Steady-state fluorescence spectra of the SBC micelles were obtained using an F-7000 spectrophotometer (Hitachi, Tokyo, Japan) with a bandwidth of 2.5 nm and λ_em of 373 nm. Pyrene was used as the probe, and the final pyrene concentration was about 5 × 10^-7 M. The morphology of the prepared SBC micelles was observed using a JEOL JEM-2100 electron microscope (TEM, JEOL Ltd., Tokyo, Japan) operating at an accelerating voltage of 200 kV.

All prepared samples were then placed in a 5 mm NMR tube and measured using a 700-MHz Bruker AVII spectrometer operating at 16.4 T, equipped with a 1H (13C/15N) TCI cryoprobe, as described previously (Chan et al., 2020 (link)). Plasma and liver spectra were acquired using a spin-echo sequence (Carr-Purcell-Meiboom-Gill [CPMG]), 32 data collections, an acquisition time of 1.5 s, a relaxation delay of 2 s, and a fixed receiver gain. Brain spectra were acquired with a Nuclear Overhauser Effect Spectroscopy (NOSEY) sequence as previously described (Chan et al., 2020 (link)). All samples were run within 9-h of being thawed.

All NMR spectra were acquired using a 700-MHz Bruker AVII spectrometer operating at 16.4 T equipped with a ¹H (¹³C/¹⁵N) TCI cryoprobe. Sample temperature was stable at 310 K. ¹H NMR spectra were acquired using a 1D NOESY presaturation scheme for attenuation of the water resonance with a 2 s presaturation. A spin-echo Carr-Purcell-Meiboom-Gill (CPMG) sequence with a τ interval of 400 μs, 80 loops, 32 data collections, an acquisition time of 1.5 s, a relaxation delay of 2 s, and a fixed receiver gain was used to supress broad signals arising from large molecular weight plasma components. CPMG spectra provide a measurement of small molecular weight metabolites and mobile side chains of lipoproteins in the plasma sample and were used for all further analysis. Due to their large molecular weight, AQP4 and MOG-IgG antibody resonances are not observed in the ¹H CPMG spectra. ¹H correlation spectroscopy (COSY) spectra were acquired on at least one sample in each classification to aid in metabolite identification. For quality control, pooled plasma samples were spread throughout the run to monitor technical variation.