Cells were fed via continuous introduction of media into the Bioreactor, resulting in a passive removal of waste into the waste bag. Cells were fed according to manufacturer's recommendations. Media was first fed at a rate of 0.1 mL/minute (min). Glucose and Lactate measurements were typically measured twice daily (Aviva Accu-Chek meter, Roche Diagnostics, Indianapolis, IN, USA and LactatePlus Lactate Meter, Nova Biomedical, Waltham, MA, USA, respectively). Once the lactate concentration reached 4 mM, the inlet rate was doubled. During the first passage, the inlet rate was doubled each time the lactate concentration rose to 4 mM until the inlet rate reached 0.4 mL/min. At this inlet rate, cells were harvested when the lactate concentration remained above 4 mM for 24-48 hours. For the second and subsequent passages, the inlet rate was doubled as before, but cells were harvested when the rate reached 1.6 mL/min and the lactate concentration reached 8 mM.
Accu chek aviva meter
Manufactured by Roche
Sourced in United States
The Accu-Chek Aviva is a blood glucose monitoring system. It is designed to measure the amount of glucose in a person's blood sample. The device provides blood glucose readings and is intended for use by people with diabetes to help manage their condition.
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7 protocols using accu chek aviva meter
1
Bioreactor-Based Cell Culture Protocol
2
Comprehensive Metabolic Profiling of Mice
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Body composition of mice was analysed prior to cull by quantitative magnetic resonance (EchoMRI 900). Energy expenditure was measured via indirect calorimetry using CLAMS (Columbus Instruments) for 10- to 12-week-old male mice. Mice were allowed to acclimatise to the cages for 2 days, prior to an average of 5 days of recordings being collected. Recording of body temperature and activity was carried out via surgically implanted radiotelemetry devices (TA-F10, Data Sciences International). Data is shown as a representative day average of single-housed age-matched males. For the diet challenge, male mice were fed HFD (60% energy from fat; DIO Rodent Purified Diet, IPS Ltd) for a period of 10–16 weeks from 12 weeks of age. Blood glucose was measured from tail blood using the Aviva Accuchek meter (Roche). For the insulin tolerance test, mice were fasted from ZT0, then injected with 0.75 IU/kg human recombinant insulin (I2643, Sigma-Aldrich) at ZT6 (time ‘0 min’).
3
Glucose Tolerance Test in Mice
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After 11 weeks of treatment, mice were fasted overnight (9 h). They were then gavaged with 1 g/kg body mass D-glucose solution and their blood glucose concentrations were measured using a glucometer (ACCU-CHEK Aviva meter, Roche, USA), 0, 15, 30, 60, and 120 min afterwards.
4
Insulin-Induced Oxytocin Response in Rats
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In another group of eight rats, anaesthetised as above but without tracheal cannulation or a gavage tube, blood samples were taken before and after intravenous (i.v.) injection of insulin to measure plasma concentrations of oxytocin and glucose. Human recombinant insulin solution (cat no. I9278; Sigma‐Aldrich Company Ltd.) was diluted in 0.9% saline (B. Braun) at 0.25 U/100 μL and injected i.v. at a dose of 0.75 U/kg bodyweight. One sample (0.6.ml ) was taken before insulin injection, and two further samples were taken at 30 and 60 min after injection. Blood glucose concentrations were measured in 50 μL of plasma using an Accu‐Chek Aviva meter (Roche Diagnostics GmbH); oxytocin was measured in duplicate in 25‐μL aliquots of plasma.
The dose of insulin given was the same as used by Paiva and Leng,
9 (link) which had been selected as a commonly used “low” dose of insulin, apparently commonly designated as such for its moderate effect on plasma glucose concentrations. In the study of Paiva and Leng, this dose reduced plasma glucose concentrations by about 50% over the following 60 min.
The dose of insulin given was the same as used by Paiva and Leng,
9 (link) which had been selected as a commonly used “low” dose of insulin, apparently commonly designated as such for its moderate effect on plasma glucose concentrations. In the study of Paiva and Leng, this dose reduced plasma glucose concentrations by about 50% over the following 60 min.
5
Metabolic Profiling of Cell Cultures
6
Fasting Glucose, Insulin, and Sensitivity Assessment
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The concentration of fasting plasma glucose (FPG) was measured by using the Accu-Chek Aviva meter (Roche, Shanghai, China). The concentration of fasting insulin (FINS) was determined by using the Mercodia Insulin ELISA Kit (cat. no. 10-1113-01; Mercodia, Uppsala, Sweden) according to the manufacturer’s procedure. Insulin sensitivity index (ISI) was calculated: ISI = 1/FPG (mmol·L-1) x FINS (mU·L-1). Homeostasis model assessment was used to calculate insulin resistance index (IRI): IRI = [FPG (mmol·L-1) x FINS (mU·L-1)]/22.5.
7
Measuring Metabolic Biomarkers in Mice
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Blood glucose levels were measured with an ACCU-CHEK aviva meter (Roche Diagnostics). Serum insulin levels were measured using the Mouse Insulin ELIZA Kit (Morinaga Institute of Biological Science). Serum glucagon levels were measured using a Bio-Plex system (Bio-Rad laboratories). Serum ferritin levels were measured using the mouse ferritin ELIZA kit (Abcam). Serum iron levels and TIBC were measured according to the Nitroso-PSAP Method [23 ]. Unsaturated iron-binding capacity (UIBC) was calculated by the difference between serum iron and TIBC.
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