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Cobas taqman hiv1 v2.0 assay

Manufactured by Roche
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Sourced in France

The Cobas TaqMan HIV1 v2.0 assay is a laboratory diagnostic tool designed to detect and quantify the human immunodeficiency virus type 1 (HIV-1) in human plasma or serum samples. The assay utilizes real-time PCR technology to provide accurate and reliable results for the monitoring of HIV-1 viral load in clinical settings.

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Liaison xl, by DiaSorin (1 mentions)

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Cobas TaqMan (73 citations) COBAS TaqMan assay (36 citations) TaqMan assay (11 citations)

2 protocols using cobas taqman hiv1 v2.0 assay

1

Quantification of HIV and CMV in Plasma

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HIV-RNA was quantified in plasma by real-time RT-PCR with the Cobas TaqMan HIV1 v2.0 assay (Roche Diagnostics, Meylan, France). Total HIV-DNA was quantified by ultra-sensitive real-time PCR in PBMC using the Generic HIV-DNA assay from BioCentric (Bandol, France).39 (link) CMV viremia was quantified by an in-house real-time PCR.40 (link) Eight samples were excluded because of insufficient volumes of plasma. CMV immunoglobulins G were determined with the automated assay on LIAISON XL (DiaSorin). Results were expressed in arbitrary units.
Gondois-Rey F., Chéret A., Granjeaud S., Mallet F., Bidaut G., Lécuroux C., Ploquin M., Müller-Trutwin M., Rouzioux C., Avettand-Fenoël V., Moretta A., Pialoux G., Goujard C., Meyer L, & Olive D. (2017). NKG2C+ memory-like NK cells contribute to the control of HIV viremia during primary infection: Optiprim-ANRS 147. Clinical & Translational Immunology, 6(7), e150-.
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2

Quantifying HIV-RNA and DNA Levels

Cited 1 time
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Human immunodeficiency virus-RNA was quantified in plasma by real-time RT-PCR with the Cobas TaqMan HIV1 v2.0 assay (Roche Diagnostics). Threshold values were arbitrarily given to samples below the threshold of the assay (20 RNA copies/mL). Total HIV-DNA was quantified by ultra-sensitive real-time PCR in PBMC using the Generic HIV-DNA assay from BioCentric (Bandol, France) as described (24 (link)).
Gondois-Rey F., Chéret A., Mallet F., Bidaut G., Granjeaud S., Lécuroux C., Ploquin M., Müller-Trutwin M., Rouzioux C., Avettand-Fenoël V., De Maria A., Pialoux G., Goujard C., Meyer L, & Olive D. (2017). A Mature NK Profile at the Time of HIV Primary Infection Is Associated with an Early Response to cART. Frontiers in Immunology, 8, 54.
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