Adrenaline bitartrate

(−)-Adrenaline bitartrate, (−)-isoprenaline hydrochloride, prazosin hydrochloride, yohimbine hydrochloride, (±)-propranolol, α-methylDOPA, Dulbecco’s Modified Eagle’s Medium/F-12 supplemented with NaHCO₃, Dulbecco’s Modified Eagle’s Medium with NaHCO₃ and stable L-glutamine, epidermal growth factor, human insulin, hydrocortisone, ICI 118,551, penicillin/streptomycin and HPLC-ECD standards from the highest purity available, were from Sigma-Aldrich (Sintra, Portugal). ITaq^TM Universal SYBR Green supermix was from Biorad (Amadora, Portugal). Foetal bovine serum was from Biochrom and L-glutamine was from Gibco (Biotecnómica, São Mamede, Portugal).
Goat anti-mouse IgG conjugated with Alexa Fluor 488 (a11029) and mouse monoclonal anti-phenylethanolamine N-methyltransferase (MA5-25530) were from Thermo Fisher Scientific (Loures, Portugal). Rabbit polyclonal anti-β₂-adrenoceptor (13096-1-AP) was from Proteintech (Rosemont, IL, USA). Goat anti-rabbit IgG conjugated with Alexa Fluor 594 (ab150092) and rabbit monoclonal anti-tyrosine hydroxylase (ab137869) were purchased from Abcam (Cambridge, UK). Goat anti-rabbit IgG conjugated with horseradish peroxidase (sc-2004) was from Santa Cruz Biotechnology Inc. (Frilabo, Maia, Portugal).

TMZ, adrenaline bitartrate, 5,5-dithio-bis-(2-nitrobenzoic acid) (DTNB), and Vanillin were purchased from Sigma-Aldrich, St. Louis, MO, USA. Trypsin and Triton-X 100 were purchased from Himedia lab Pvt. Ltd. (Mumbai, India). Carboxymethyl cellulose (CMC) was purchased from Rankem, India. Sodium Chloride and trichloroacetic acid were purchased from SD Fine Chemicals, India. Disodium EDTA, hydrogen peroxide, disodium hydrogen phosphate dehydrate sodium bicarbonate, potassium dihydrogen phosphate, and disodium hydrogen phosphate were purchased from Himedia Laboratories Ltd, India. Fetal bovine serum and Dulbecco’s modified Eagle’s medium were purchased from Gibco, USA.

(−)-Adrenaline bitartrate, (−)-isoprenaline hydrochloride, salbutamol, atenolol, L-748,337, ESI-09, forskolin, 3-Isobutyl-1-methylxanthine (IBMX), 8-Bromoadenosine 3′,5′-cyclic monophosphate (8-Br-cAMP), H-89 dihydrochloride hydrate, Nile Red and penicillin/streptomycin were obtained from Sigma-Aldrich (Chemicalnor, Valongo, Portugal). Foetal bovine serum (FBS) was obtained from Biochrom (Biotecnómica, São Mamede, Portugal). Dulbecco’s modified Eagle’s medium—high glucose, supplemented L-Glutamine (Gibco) and 0.25% trypsin/0.025% EDTA solution (Gibco) were obtained from Alfagene (Carcavelos, Portugal).