Biochanin A (BCA), purity of 98% (Sigma Aldrich, Steinheim, Germany); potassium bromide (KBr) for IR spectroscopy, ≤100% (Merck KGaA, Darmstadt, Germany); Hanks’ buffered solution pH 7.4 GmbH (PAA Laboratories, Pasching, Austria); 2-propanol, purity of 99.5% (Centrohem, Belgrade, Serbia) and 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT), purity of ≥97.5% (Sigma Aldrich, Steinheim, Germany) were used. Semi-crystalline PLA (consisting of D, L-lactic acid units with low L-lactide content, presumably about 10%), purity of 99% provided from Shenzhen Esun Industrial Co., Ltd. (Shenzhen, China), characterized by a number-average molecular weight (Mn) of 60.520 g/mol, a weight-average molecular weight (Mw) of 160.780 g/mol, and polydispersity index (PDI) of 2.66, was used for the preparation of electrospun polymer fibrous matrices. Chloroform, purity of ≥99.5% (Lachner, Neratovice, Czech Republic) and dimethylformamide (Fischer Scientific, Waltham, USA), purity of ≥99.8% were used for the preparation of polymer-based solutions for electrospinning. All chemicals were used as received.
3 4 5 dimethylthiazol 2 yl 2 5 diphenyl 2h tetrazolium bromide
Manufactured by Merck Group
Sourced in Serbia, Germany, India, United States
3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide is a chemical compound used in cell-based assays. It is a yellow tetrazolium salt that is reduced to a purple formazan product by living cells, allowing for the quantification of cell viability and proliferation.
Automatically generated - may contain errors
Lab products found in correlation
Triton x 100, by Merck Group (2 mentions)
Dimethylformamide, by Thermo Fisher Scientific (1 mentions)
Chloroform, by Lach-Ner (1 mentions)
Potassium bromide kbr for ir spectroscopy, by Merck Group (1 mentions)
Hanks buffered solution ph 7.4 gmbh, by GE Healthcare (1 mentions)
Fetal bovine serum (fbs), by Merck Group (1 mentions)
Streptomycin, by Merck Group (1 mentions)
Penicillin, by Merck Group (1 mentions)
Glutamine, by Merck Group (1 mentions)
Dimethyl sulfoxide (dmso), by Vetec (1 mentions)
Rpmi 1640 medium, by Merck Group (1 mentions)
Methanol, by Vetec (1 mentions)
Microplate reader, by Agilent Technologies (1 mentions)
Dmem f12, by Thermo Fisher Scientific (1 mentions)
Hi fbs, by Thermo Fisher Scientific (1 mentions)
Minimum essential medium (mem), by Thermo Fisher Scientific (1 mentions)
Kanamycin, by Thermo Fisher Scientific (1 mentions)
Non essential amino acids (neaa), by Euroclone (1 mentions)
Pen strep, by Euroclone (1 mentions)
Na pyruvate, by Euroclone (1 mentions)
9 protocols using 3 4 5 dimethylthiazol 2 yl 2 5 diphenyl 2h tetrazolium bromide
1
Biochanin A Electrospun PLA Fibrous Matrices
2
Cytotoxicity Evaluation of Doxorubicin
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Methanol, dimethyl sulfoxide (DMSO), polisorbate, formaldehyde,sodium chloride (NaCl), potassium chloride (KCl), and calcium chloride (CaCl2) were purchased from Vetec Química Fina Ltda (Rio de Janeiro, Brazil). Glutamine,RPMI 1640 medium, fetal bovine serum, penicillin,streptomycin, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT), and Triton X-100 wereobtained from Sigma-Aldrich (Germany). Carbon dioxide (CO2) was from White Martins (Sao Paulo, Brazil) and doxorubicin (Doxolem) from Zodiac Produtos Farmacêuticos S/A (São Paulo, Brazil).
3
Determining Cell Viability Using MTT Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
To determine cell viability, the HNSCC cell lines and HaCaT cells were seeded onto 96-well plates at densities of 5×103 cells/well in 1 mL complete medium with various concentrations of luminacin (0-50 μg/mL). MTT, also known as 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (Sigma-Aldrich), was added to 40 μL of the cell suspension for 4 hours. After three washes with phosphate buffered saline (PBS, pH 7.4), the insoluble formazan product was dissolved in dimethyl sulfoxide (DMSO). The optical density of each culture well was measured using a microplate reader (Bio-Tek, Winooski, VT) at 540 nm.
4
Propagation and Infection Assay of hCoV-229E
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
hCoV229E (ATCC® VR-740™) was in propagated in MRC-5 cells (ATCC:CCL-171™) maintained in MEM (Gibco) supplemented with 10% v/v fetal beef serum (FBS HI; Gibco), 1 mM Na Pyruvate (Euroclone), 1 mM Non Essential Amino Acids (Euroclone) and 1x Pen-strep (Euroclone) and kept under 5% CO2 on 37 °C. BEAS-2B cells were kindly provided by Pierre-Olivier Vidalain were maintained in DMEM/F-12 (Gibco), 5% FBS HI (Gibco), 1% Kanamycin (Thermo-Fisher Scientifics), at 37 °C with 5% CO2. 2*104 cells per well were seeded in transparent 96 well plate and incubated over night in order to reach 90% confluency. 24 h later cells were infected with hCoV-229E m. o.i. 0.06 in DMEM/F-12 in presence of compound or 0,1% DMSO (untreated controls). The cells were incubated for 2 h at 35 °C with 5% CO2, then the virus was removed, replaced with complete medium with or without compound, and incubated at 35 °C with 5% CO2. After 72 h, 20 μL of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (Sigma-Aldrich) dissolved in PBS at 7,5 mg/mL were added to each well and incubated at 37 °C with 5% CO2 for 1 h. Then the supernatant was removed and cells were lysed with 100 μL/well of: 10% 2-Propanol, 0,004% Triton-X-100 (Sigma-Aldrich), 0,0004% HCl, then the absorbance was read at 570 nm with a plate reader Victor Nivo5 PerkinElmer.
5
Immunofluorescence Staining Antibody Panel
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Anti-cyclin A (Cat # sc-751), anti-cyclin B1 (Cat # sc-752), anti-cyclin E (Cat # sc-198), anti-Ki67 (Cat # sc-101861), anti-myogenin (Cat # sc-12732) and anti-MyoD (Cat # sc-304) were from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Anti-PHB2 (Cat # AB10198) was obtained from Millipore (Darmstadt, Germany). Anti-c-Myc (Cat # 13987) were from Cell Signaling Technology (Danvers, MA, USA). Anti-B23 (nucleophosmin/NPM, Cat # B0556) was purchased from Sigma. The mouse monoclonal antibody against GAPDH was purchased from Kangcheng Bio-tech (Shanghai, China). 4,6-diamidino-2-phenylindole (DAPI) and Cell Cycle Analysis Kits were obtained from Beyotime (Shanghai, China). The Cell Proliferation ELISA Kit (5-bromo-20-deoxyuridine, BrdU) was purchased from Roche Diagnostics (Indianapolis, IN, USA). 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2Htetrazolium bromide (MTT) was purchased from Sigma.
6
Cell Proliferation and Viability Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For proliferation and viability experiments, 1500 and 50,000 cells were seeded in 96-well plates, respectively. Proliferation was assayed in cells grown overnight in DMEM containing 1% FBS followed by 48 h in DMEM with 10% serum. Viability was assessed in cells left overnight in DMEM with 1% FBS followed by 48 h in serum-free media. Cells grown in DMEM containing 1% FBS served as controls. At the end of the experiment, cells were incubated with 110 μl of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (50 μg/ml; Sigma–Aldrich) for 4 h at 37 °C. Subsequently, 100 μl of dimethyl sulfoxide was added, and absorbance was measured at 595 nm.
7
Antioxidant and Cytotoxicity Evaluation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Trifluoroacetic acid (TFA), monosaccharide standards (glucose, galactose, rhamnose, mannose, xylose, and arabinose), deuterium oxide (D2O), L-ascorbic acid, 2-diphenyl-1-picrylhydrazyl (DPPH), 2, 2’-azino-bis 3-ethylbenzothiazoline-6-sulfonic acid (ABTS), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT), Acridine orange/ethidium bromide (AO/EB) and 4’,6-diamidino-2-phenylindole (DAPI) were procured from Sigma-Aldrich (Bangalore, India). Further, sodium nitroprusside, benzene, n-Hexane, n-Hexadecane, toluene, and xylene were purchased from Sisco Research Laboratories (Mumbai, India). Kaolin clay, Nutrient agar, DMEM (Dulbecco’s Modified Eagle’s Medium), FBS (Fetal Bovine Serum) and absolute alcohol were obtained from HiMedia (Mumbai, India). All chemicals and reagents used were of analytical and ultrapure grade.
8
Cell Culturing and Assay Reagents
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Dulbecco’s modified Eagle’s medium/F12 nutrient mixture Ham (DMEM/F12 3:1), antibiotic–antimycotic solution, trypsin–EDTA, phosphate buffered saline, Hank’s Balanced Salt Solution (HBSS) and fetal bovine serum (FBS) were purchased from Gibco (Grand Istand, NY, USA). 3-(4,5-Dimethylthiazol-2-yl)-2,5 Diphenyl-2H-tetrazolium Bromide (MTT), phosphoramidon disodium salt (Phosphoramidon), ( +) sodium l -ascorbate (l -Ascorbate), epigallocatechin gallate (EGCG), N-succinyl-tri-alanyl-p-nitroanilide (STANA), albumin from bovine serum (BSA), Bradford reagent and trifluoroacetic acid (HPLC grade) were obtained in Sigma-Aldrich (St. Louis, MO, USA). Besides, dimethyl sulfoxide (DMSO) was purchased from Amresco (Solon, OH, USA). Triton X-100 was obtained from Yakuri Pure Chemicals (Tokyo, Japan). Ethanol (HPLC grade) was obtained from Duksan Chem. Co. (Seoul, South Korea). Acetonitrile (HPLC grade) was purchased from Fisher Scientific Korea (Seoul, South Korea). Procollagen type I peptide (PIP) ELISA kit was obtained from Takara (Tokyo, Japan). Matrix Metalloproteinase-1 (MMP-1) Human ELISA kit was purchased from Abcam (Cambridge, MA, USA). YOKUDELNA calibration kit was obtained from JEOL Ltd. (Tokyo, Japan).
9
Phytochemical and Bioactivity Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All reagents were of analytical grade and used without further purification. The organic solutions of normal alkanes were purchased from Fluka (Buchs, Switzerland). Gallic acid, ascorbic acid, α-tocopherol, butylated hydroxytoluene (BHT), β-carotene, linoleic acid and tween 40 were all provided by Merck.
An SPME device with all its accessories and consisting of a polymeric fiber (polydimethylsiloxane: PDMS) with general diameter of 75-µm was provided by Supelco (Bellefonte, PA, USA). For the evaluation of antibacterial activities, all the microorganisms were obtained from the Microbiology Laboratory Culture Collection (MLCC) of Sabzevar Medical Science University. For the cytotoxicity study, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2Htetrazolium bromide (MTT) was purchased from Sigma-Aldrich. KB and NIH3T3 cell lines were provided from Shahrood University of Medical Sciences, Khatamolanbia Hospital, while HUVECs cell line was supplied from Ilam University of Medical Sciences.
An SPME device with all its accessories and consisting of a polymeric fiber (polydimethylsiloxane: PDMS) with general diameter of 75-µm was provided by Supelco (Bellefonte, PA, USA). For the evaluation of antibacterial activities, all the microorganisms were obtained from the Microbiology Laboratory Culture Collection (MLCC) of Sabzevar Medical Science University. For the cytotoxicity study, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2Htetrazolium bromide (MTT) was purchased from Sigma-Aldrich. KB and NIH3T3 cell lines were provided from Shahrood University of Medical Sciences, Khatamolanbia Hospital, while HUVECs cell line was supplied from Ilam University of Medical Sciences.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!