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2 protocols using bioresmethrin

1

Insecticide Screening for Voltage-Sensitive Sodium Channels

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A total of 20 insecticides that target the VSSC and two synergists were used in this study: acrinathrin (88.7%, Chem Service, West Chester, PA, USA), bioallethrin (3.8% cis, 95.7% trans, Chem Service), bioresmethrin (99.5%, Chem Service), cispermethrin (99%, Chem Service), cyfluthrin (98%, Bayer CropScience, Leverkusen, Germany), cyhalothrin (90.2%, ICI Americas, Wilmington, DE, USA), cypermethrin (94.6%, ICI Americas), DDT (1,1‐bis(4‐chlorophenyl)‐2,2,2‐trichloroethane) (98%, Sigma‐Aldrich, St. Louis, MO, USA), deltamethrin (99.5%, Chem Service), diethyl maleate (DEM) (97%, Sigma‐Aldrich), etofenprox (96.3%, Mitsui Toatsu, Tokyo, Japan), 1R‐trans fenfluthrin (100%, Bayer CropScience), fenpropathrin (99.5%, Chem Service), flumethrin (100%, Bayer CropScience), indoxacarb (DPX‐MP062, 100%), and its bioactive metabolite DCJW, (98%, DuPont, Wilmington, DE, USA), tau‐fluvalinate (93.8%, Chem Service), permethrin (99.5% pure, 24.1% cis, 75.8% trans, Chem Service), piperonyl butoxide (PBO) (90%, Sigma‐Aldrich), tefluthrin (96%, Sigma‐Aldrich), transfluthrin (99.5%, Chem Service) and trans‐permethrin (99% FMC, Philadelphia, PA, USA). All insecticides were diluted in acetone (VWR, Radnor, PA, USA).
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2

Insecticides and Synergist Evaluation

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A total of 20 insecticides (representing diverse structures and known stereochemical composition) and three synergists were used (Fig 3): 1R-cis αS cypermethrin (100%, Bayer CropScience, Leverkusen, Germany), 1R-trans fenfluthrin (100%, Bayer CropScience), alpha-cypermethrin (99.5%, Chem Service, West Chester, PA, USA), bioallethrin (3.8% cis and 95.7% trans, Chem Service), bioresmethrin (99.5%, Chem Service), cis-permethrin (99.4%, Zeneca Ag products), cyfluthrin (99.2%, Chem Service), cyhalothrin (90.2%, ICI Americas), cypermethrin (44.8% cis and 53.9% trans, Chem Service), N-decarbomethoxylated JW062 (DCJW) (98%, DuPont), DDT (98%, Sigma-Aldrich, St. Louis, MO, USA), deltamethrin (99.5%, Chem Service), diethyl maleate (DEM) (97%, Sigma-Aldrich), etofenprox (96.3%, Mitsui Toatsu Chemicals, Tokyo Japan), flumethrin (100%, Bayer CropScience), permethrin (24% cis and 76% trans, Chem Service), piperonyl butoxide (PBO) (90%, Sigma-Aldrich), S,S,S-tributyl phosphorotrithioate (DEF) (97.3%, Chem Service), tau-fluvalinate (96%, Chem Service), tefluthrin (96%, Sigma-Aldrich), transfluthrin (99.5%, Chem Service), trans-permethrin (99%, FMC, Philadelphia, PA, USA).
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