Interleukin 6 (il 6)

MH-S and HepG2 cells were obtained from Procell Life Science & Technology Co., Ltd. (Wuhan, China); kaempferol 3-O-α-L-rhamnoside-7-O-β-D-xylosyl(1→2)-O-α-L-rhamnoside (PubChem CID: 74978103, SGPF) was obtained from the TCM and Ethnomedicine Innovation & Development International Laboratory Innovative Materia Medica Research Institute, School of Pharmacy, Hunan University of Chinese Medicine (Changsha, China), the extract was identified by Prof. Wei Wang, and a specimen (no. 20200911) was deposited in the lab; RPMI1640 Medium (RP-1640), MEM (obtaining NEAA), fetal bovine serum (FBS), trypsin, penicillin/streptomycin, and PBS were obtained from Procell (Wuhan, China); lipopolysaccharides (LPSs) were obtained from Sigma (St. Louis, MO, USA); oil acid (OA) was obtained from Macklin Co. Ltd. (Shanghai, China); CCK-8 was purchased from Seven Biotech (Beijing, China); RIPA buffer and PMSF were purchased from Solarbio (Beijing, China); TNF-α, IL-1β, IL-6, and IL-10 ELISA kits were purchased from Bioswamp (Wuhan, China); and a tissue total cholesterol assay kit, tissue total triglyceride assay kit, liquid sample MDA assay kit, and tissue SOD assay kit were purchased from Applygen Technologies Co., Ltd. (Beijing, China).

Human serum HMGB1 were quantified by the HMGB1 ELISA Kit (Elabscience, Wuhan, China). After anesthesia, mouse blood samples were obtained through the retro-orbital sinus. Serums were collected by centrifugation at 2,500 rpm at 4°C for 15 min for ELISA test. IL-6 (Bio-swamp, Wuhan, China), TNF-α (Bio-swamp, Wuhan, China), HMGB1 (Elabscience, Wuhan, China) were quantitatively detected according to the instructions.