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Iberiotoxin

Manufactured by Alomone
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Sourced in Israel

Iberiotoxin is a selective blocker of large-conductance calcium-activated potassium (BK) channels. It is a purified protein isolated from the venom of the scorpion Buthus tamulus.

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Lab products found in correlation

Apamin, by Alomone (4 mentions) Stromatoxin, by Alomone (3 mentions) Ns1619, by Alomone (2 mentions) Tetrodotoxin, by Alomone (2 mentions) Charybdotoxin, by Alomone (2 mentions) Methoxamine, by Merck Group (2 mentions) Xe991, by Bio-Techne (2 mentions) Acetylcholine, by Merck Group (2 mentions) Cd29 alexa fluor 488, by BioLegend (1 mentions) Veratridine, by Alomone (1 mentions) Ionomycin, by Cayman Chemical (1 mentions) Eht1864, by Santa Cruz Biotechnology (1 mentions) Phenytoin, by Merck Group (1 mentions) Bradykinin, by Merck Group (1 mentions) Serotonin, by Merck Group (1 mentions) Collagenase, by Merck Group (1 mentions) Trypsin inhibitor, by Merck Group (1 mentions) Elastase, by Serva Electrophoresis (1 mentions) Dl dithiothreitol, by Merck Group (1 mentions) 5 hmf, by Merck Group (1 mentions)

Close spelling variants of this product

Iberiotoxin (IbTX) (3 citations)

14 protocols using iberiotoxin

1

Characterizing KCa Channel Regulation of IMA Contraction

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Following a 40-min equilibration period, cumulative increasing concentrations of the thromboxane A2 (TXA2) mimetic U46619 (−11–−4.5 LogM) (Cayman Chemical, Cat#16450) were added in the myograph chamber to generate a concentration-response curve. The role played by each KCa channel subtype in the regulation of IMA constriction was further characterized by comparing a first concentration-response curve constructed in control conditions with a second concentration-response curve constructed in the same ring segment after a selective inhibition of the subtype. The BKCa subtype blocker iberiotoxin (100 nmol/L) (Alomone labs, Cat#STI-400) (group Ia), the IKCa subtype blocker TRAM-34 (1 µmol/L) (Alomone labs, Cat#T-105) (group Ib), or the SKCa subtype blocker apamin (100 nmol/L) (Alomone labs, Cat#STA-200) (group Ic) were added individually or in combination (group Id: TRAM-34 + apamin, group Ie: iberiotoxin + TRAM-34 + apamin) 30 min prior to contraction with U46619. For vessels pretreated with TRAM-34 + apamin, or iberiotoxin + TRAM-34 + apamin, the drugs were added in a randomized order.
Sun W.T., Xue H.M., Hou H.T., Chen H.X., Wang J., He G.W, & Yang Q. (2021). Homocysteine alters vasoreactivity of human internal mammary artery by affecting the KCa channel family. Annals of Translational Medicine, 9(8), 625.
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2

BK Channel Modulator Protocol

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The BK channel antagonist, Iberiotoxin (IBTX), and agonist, NS1619 (Alomone Labs, Jerusalem, Israel), were solubilized in water and dimethyl sulfoxide, respectively. CD45-allophycocyanin (APC), CD90-phycoerythrin (PE), and CD29-Alexa Fluor 488 conjugated antibodies were purchased from Biolegend, San Diego, CA, United States. Anti-sloβ1 (NB300-535) and anti-KCa1.1 (NBP1-46701), directed against β1 and α subunits, respectively, were from Alomone Labs and Novus biological. Anti-rabbit Alexa Fluor 488 was purchased from Invitrogen, Carlsbad, CA, United States.
Echeverry S., Grismaldo A., Sánchez C., Sierra C., Henao J.C., Granados S.T., Sutachán J.J, & Torres Y.P. (2020). Activation of BK Channel Contributes to PL-Induced Mesenchymal Stem Cell Migration. Frontiers in Physiology, 11, 210.
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3

Ion Channel Pharmacology Protocols

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Iberiotoxin, NS‐1619, tetrodotoxin (TTX) and veratridine were purchased from Alomone Labs. Phenytoin was from Sigma. Ionomycin was from Cayman Chemical. EHT1864 was from Santa Cruz Biotechnology. Drugs were reconstituted as stock solutions according to manufacturer guidelines and diluted directly into culture medium and/or recording solutions at the indicated working concentrations.
Yang M., James A.D., Suman R., Kasprowicz R., Nelson M., O'Toole P.J, & Brackenbury W.J. (2019). Voltage‐dependent activation of Rac1 by Nav1.5 channels promotes cell migration. Journal of Cellular Physiology, 235(4), 3950-3972.
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4

Enzymatic Protein Extraction Protocol

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Serotonin, bradykinin, albumin, collagenase, trypsin inhibitor, DL-dithiothreitol, and the salts for the solutions were obtained from Sigma. Papain was purchased from FERAK (Berlin, Germany). Elastase was from Serva, and iberiotoxin and stromatoxin were from Alomone Labs.
Här K., Lysenko N.N., Dimitrova D., Schlüter T., Zavaritskaya O., Kamkin A.G., Mladenov M., Grisk O., Köhler R., Gagov H, & Schubert R. (2023). Kv2.1 Channels Prevent Vasomotion and Safeguard Myogenic Reactivity in Rat Small Superior Cerebellar Arteries. Cells, 12(15), 1989.
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5

Measuring Mitochondrial Function and BK Ca Channel

Cited 1 time
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Mitochondrial respiration and ΔΨ were measured in isolated endothelial mitochondria as previously described [11 (link),64 (link)]. Oxygen uptake was determined polarographically using a Rank Bros. (Cambridge, UK) oxygen electrode in 2.8 ml of incubation medium (50 mM KCl, 70 mM sucrose, 2.5 mM KH2PO4, 2 mM MgCl2, 10 mM HEPES, 10 mM Tris/HCl (pH 7.2), and 0.05% BSA) with 2 mg of mitochondrial protein (at 37 °C). Mitochondrial ∆Ψ was measured simultaneously with oxygen uptake using a tetraphenylphosphonium (TPP+)-specific electrode. Measurements were made in the presence of 5 mM succinate (as respiratory substrate), 4 µM rotenone (to inhibit complex I), 2 µg oligomycin (to inhibit ATP synthase), 1.7 µM carboxyatractyloside (to inhibit ATP/ADP antiporter), and 0.15 mM ATP (to activate succinate dehydrogenase). Up to 2 µM iberiotoxin (dissolved in water; Alomone Labs, Israel) or 0.3 mM paxilline (dissolved in methanol; Merck, Poznan, Poland) were used to inhibit the mitoBKCa channel.
Kicinska A., Kampa R.P., Daniluk J., Sek A., Jarmuszkiewicz W., Szewczyk A, & Bednarczyk P. (2020). Regulation of the Mitochondrial BKCa Channel by the Citrus Flavonoid Naringenin as a Potential Means of Preventing Cell Damage. Molecules, 25(13), 3010.
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6

Pharmacological Inhibitors of Potassium Channels

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1H‐[1,2,4]oxadiazolo‐[4,3‐a]quinoxalin‐1‐one (ODQ) was obtained from Enzo Life Sciences (Lausen, Switzerland) purchased through Eubio (Vienna, Austria). Apamin, charybdotoxin and iberiotoxin were purchased from Alomone Labs (Jerusalem, Israel). Collagenase (Worthington CLS 2) was from Worthington Biochemical Corporation (New York, USA). All other chemicals, including 5‐HMF, were of standard grade and purchased from Sigma‐Aldrich (Vienna, Austria).
Wölkart G., Schrammel A., Koyani C.N., Scherübel S., Zorn‐Pauly K., Malle E., Pelzmann B., Andrä M., Ortner A, & Mayer B. (2017). Cardioprotective effects of 5‐hydroxymethylfurfural mediated by inhibition of L‐type Ca2+ currents. British Journal of Pharmacology, 174(20), 3640-3653.
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7

Pharmacological Modulators of Ion Channels

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Tetraethylammonium (TEA) was purchased from Santa Cruz Biotech, California, USA. Iberiotoxin was purchased from Alomone, Jerusalem, Israel. Glibenclamide was purchased from Research Biochemical International, Massachusetts, USA. Tetrodotoxin (TTX) was purchased from Tocris Bioscience, Bristol, UK. ω-conotoxin GVIA (CTX) was purchased from Bachem, Bubendorf, Switzerland. KT 5720, KT 5823, NG-Nitro-L-arginine (L-NNA), apamine, papaverine, and resveratrol were obtained from Sigma-Aldrich, Missouri, USA. Charybdotoxin was purchased from AnaSpec Inc., California, USA.
Tsai C.C., Lee M.C., Tey S.L., Liu C.W, & Huang S.C. (2017). Mechanism of resveratrol-induced relaxation in the human gallbladder. BMC Complementary and Alternative Medicine, 17, 254.
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8

Sperm Preparation and Fluorescent Dyes

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L. pictus sea urchins were obtained from Marinus (Long Beach, CA, USA). Dry sperm were collected after intracelomic injection of 0.5 M KCl and kept on ice until used. The fluorescent dyes 3,3'-dipropylthiadicarbocyanine iodide (DiSC3(5)), Fluo-4-AM, Quin-2 and 5-(and-6)-Carboxyfluorescein diacetate were obtained from Molecular Probes (Eugene, OR, USA). Anhydrous dimethylsulfoxide (DMSO), tolbutamide, glibenclamide, were from Sigma-Aldrich. ZnSO4 was from Merck. Charybdotoxin and Iberiotoxin were from Alomone Labs. The Kits to measure cAMP (TRK 432) and cGMP (TRK 500) were from Amersham. Speract was synthesized in Professor Possani's Laboratory (IBT-UNAM) and fucose sulfate polymer (FSP) was prepared according to the previous report (Garbers et al., 1983 (link)). The rest of the reagents used were of the highest quality available.
Beltrán C., Rodríguez-Miranda E., Granados-González G., de De la Torre L.G., Nishigaki T, & Darszon A. (2014). Zn2+ induces hyperpolarization by activation of a K+ channel and increases intracellular Ca2+ and pH in sea urchin spermatozoa. Developmental biology, 394(1), 15-23.
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9

Characterizing K+ Channel Blockers

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Drugs used were 0.1 µM iberiotoxin (Alomone Labs, Israel) and 0.5 µM apamin (Alomone). Working solutions containing these drugs were freshly made from stock solutions just before each experiment and were applied by a custom-made gravity-fed perfusion system, with a 250 µm internal diameter tube positioned about 300 µm from the recorded cell. When applying drugs in current-clamp mode, cells were recorded in continuous recording mode for 1 min before starting the V–I protocol. Initial experiments showed that 1 min of exposure to these blockers is sufficient to elicit the full effect.
Margas W., Ferron L., Nieto-Rostro M., Schwartz A, & Dolphin A.C. (2016). Effect of knockout of α2δ-1 on action potentials in mouse sensory neurons. Philosophical Transactions of the Royal Society B: Biological Sciences, 371(1700), 20150430.
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10

Electrophysiological Experiments Protocols

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All electrophysiological experiments were performed as described previously23 (link). Agatoxin, isradipine, SNX 482, stromatoxin and iberiotoxin were purchased from Alomone (Jerusalem, Israel) and tetrodotoxin (TTX) from Sigma (Gillingham, UK).
Hastoy B., Godazgar M., Clark A., Nylander V., Spiliotis I., van de Bunt M., Chibalina M.V., Barrett A., Burrows C., Tarasov A.I., Scharfmann R., Gloyn A.L, & Rorsman P. (2018). Electrophysiological properties of human beta-cell lines EndoC-βH1 and -βH2 conform with human beta-cells. Scientific Reports, 8, 16994.
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