For creation of the 6-OHDA lesion, the rat was anesthetized with an intraperitoneal (IP) injection of pentobarbital (50 mg/kg). The animal’s head was fixed in a stereotactic apparatus (Narishige, Tokyo, Japan). All animals received injections of a total of 8 μg of 6-OHDA (dissolved in 4 μl of 0.9% physiological saline containing 0.02% ascorbic acid [Sigma-Aldrich Co., St Louis, MO, USA]). The coordinates were calculated with reference to bregma for the anterioposterior and the mediolateral coordinates using the rat brain atlas16 as follows: 1) anterioposterior – 3.7, mediolateral – 1.7; 2) anterioposterior – 4.4, mediolateral – 1.2. The dorsoventral position of all injections was −7.8 mm below the dura and the tooth bar set to −2.4 mm. Three weeks after injections, the rats that exhibited a stable apomorphine-induced rotational asymmetry of at least seven full turns per minute away from the lesioned side were selected for the next experiment. It has been previously demonstrated that rats meeting this criterion have a greater than 90% depletion of striatal dopamine.17 (link)
Stereotactic apparatus
Manufactured by Narishige
Sourced in Japan
The Stereotactic apparatus is a precision instrument used to precisely position and orient a subject or object in three-dimensional space. It is designed to provide a stable and accurate reference frame for various research and medical applications.
Automatically generated - may contain errors
Lab products found in correlation
7 protocols using stereotactic apparatus
1
Rat 6-OHDA Lesion Model Protocol
2
Targeting Basolateral Amygdala in Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Male Scopfl/fl mice aged 8–10 weeks were deeply anesthetized with a mixture of ketamine (140 mg/kg) and xylazine (8.8 mg/kg) in bacteriostatic saline given intraperitoneally (20 ml/kg) and placed on a stereotactic apparatus (Narishige, Tokyo, Japan). The skull was exposed, and holes were drilled bilaterally above the basolateral amygdala. The coordinates relative to bregma were: anteroposterior, −1.65 mm; lateral, +3.30 mm; dorsoventral, −4.45 mm. Mice were bilaterally injected with 0.5 μl of either AAV-Cre or AAV-GFP over 5 min, and the needles were kept in place for an additional 5 min to ensure infusion.
3
Fluorescent Nerve Tracing Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Five weeks after the cross-link operation, the animals were anesthetized with pentobarbital (47 mg/kg) and clamped in a stereotactic apparatus (Narishige, Tokyo). A small burr hole was made with a dental drill in the medial part of the occipital bone and 1 μL of 10% fluoro-ruby was injected into the nucleus of the hypoglossal nerve with a Hamilton syringe wear on the glass tip (n = 3). Five days thereafter, the animals were transcardially perfused with 10% buffered formalin and their hypoglossal, facial, and grafted nerves were harvested as an “H”-shaped tissue piece (Fig. 1 D). This was immersed in 5% buffered sucrose (pH 7.4) at 4 °C overnight then in 20% buffered sucrose and frozen in a 2:1 mixture of 20% buffered sucrose-OCT compound (Sakura Tek, Japan) as previously described [8] (link). The nerves were then sliced into 10-μm-thick sections in a cryostat.
4
Rodent Motor Cortex Aspiration
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Rats were anesthetized with isoflurane (1.5%) in a mixture of O2 and N2O (50%: 50%) and clamped in a stereotactic apparatus to keep their head at the horizontal position (Narishige, Tokyo, Japan). After checking for the absence of the pain reflex by pinching the cranial skin, a small midline incision in the skin and a small window of the skull over the motor cortex was made using a drill (Minitor Co., Tokyo, Japan). The motor cortex was aspirated from −1.0 to 1.0 mm rostral to the bregma and from 0.5 to 2.5 mm lateral to the midline in 2-week-old rats with the corpus callosum left intact, and −1.5 to 1.5 mm rostral to the bregma and from 0.5 to 3.5 mm lateral to the midline in 7- and 12-week-old rats (Fig. 1a ). Body temperature was maintained in the normothermic range (37–38 °C) with a feedback-controlled heating pad and incubator (Biomachinery Co., Chiba, Japan).
5
Stereotactic VTA Implantation in Rats
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
In the present study, the rats were anesthetized with a mixture of ketamine (20 mg/kg) and azpromazine (10 mg/kg) and placed in a stereotactic apparatus (Narishige, Japan).
Scarves with a score of 23 were placed as guide cannula in the VTA core based on the atlas of Paxinos and Watson [17] . The cannulas embedded in the skull bone were fixed with the aid of a cold dental acrylic. The injection electrode was connected to the polyethylene tube and the other one was attached to a suitable Hamilton syringe (1 μl). Surgery and injections were performed in sterile conditions. After surgery, animals were recovered for at least a week and then tested. All experiments and procedures were designed and implemented based on animal protection codes and Helsinki treaties on laboratory animals were considered.
Scarves with a score of 23 were placed as guide cannula in the VTA core based on the atlas of Paxinos and Watson [17] . The cannulas embedded in the skull bone were fixed with the aid of a cold dental acrylic. The injection electrode was connected to the polyethylene tube and the other one was attached to a suitable Hamilton syringe (1 μl). Surgery and injections were performed in sterile conditions. After surgery, animals were recovered for at least a week and then tested. All experiments and procedures were designed and implemented based on animal protection codes and Helsinki treaties on laboratory animals were considered.
6
Intracranial Tumor Induction in Nude Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Immunodeficient mice were obtained from CLEA Japan, Inc. During the experimental period, the mice were kept in a cage and housed in a temperature-and humiditycontrolled aseptic room provided with a light/dark cycle system. Either U87 glioma cells or U251 Oct-3/4 GSL cells (10 5 ) were suspended in 3 μl of the culture medium and injected into the brains of 6-to 8-week-old, female, specific pathogen-free, nu/nu BALB/c mice (weight 20 ± 2 g [mean ± SD]) by using a stereotactic apparatus (Narishige). Before placing the mice in the stereotactic apparatus, the animals were anesthetized with IP medetomidine (0.75 mg/ kg), midazolam (4 mg/kg), and butorphanol tartrate (5 mg/ kg). The stereotactic coordinates of the injection site were as follows: 1 mm forward from the coronal suture, 2 mm lateral to the sagittal suture, and 3 mm deep. Preliminary MRI of the mouse brain was performed to confirm the size and location of a generated tumor just before performing SDT.
7
Hippocampal Neurophysiology during Elevated Plus Maze
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
On the day of surgery, rats were anesthetized using pentobarbital sodium at 50 mg/kg, intraperitoneally (i.p.), and restrained in a stereotactic apparatus (Narishige Co., Tokyo, Japan). The subcutaneous layer of tissue was removed to expose the skull. The implantation position (4.5 mm posterior to bregma, 3.5 mm laterally) for electrode arrays (16 channels) (Plexon Inc., Hong Kong, China) was set according the rat brain atlas drawn by the Paxinos and Watson, and then a hole at this position was drilled in the skull. A microdrive was positioned and the electrode array was lowered through the drilled hole into the CA1 of the left dorsal hippocampus (−2.5 mm relative to the brain surface, CA1 region). The gaps between the electrodes and hole were filled with softened paraffin and the microdrive was secured with dental cement. After completing the surgery, antibiotic penicillin (75,000 U) was i.p. administered for 3–5 days to prevent possible infections. One week after surgery, the EPM test was performed simultaneously with multi-channel in vivo extracellular recordings in the hippocampus CA1, 30 min after the rats received 4-OH CA (14 mg/kg), 4-Cl CA (14 mg/kg), or vehicle.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!