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Zen microscope software v2

Manufactured by Zeiss
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ZEISS ZEN microscope software v2.3 is a comprehensive image acquisition and analysis platform designed to support a wide range of microscopy techniques. It provides a user-friendly interface for controlling ZEISS microscopes and capturing high-quality images. The software offers advanced features for image processing, analysis, and data management, enabling researchers to efficiently explore and understand their samples.

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Lab products found in correlation

Axiocam hrm, by Zeiss (2 mentions) Axio imager a2 microscope, by Zeiss (2 mentions) Tunel assay kit brdu red, by Abcam (1 mentions) Proteinase k, by Thermo Fisher Scientific (1 mentions)

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ZEN software (2479 citations) ZEN microscope software (82 citations)

2 protocols using zen microscope software v2

1

Nuclei Quantification in Tissue Sections

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Tissue sections were deparaffinized, rehydrated and nuclei stained for 10 min at room temperature with 1 µg/ml Hoechst 33342. Nuclei were imaged using a fluorescent AxIo Imager.A2 microscope (Carl Zeiss AG, DE) equipped with an AxioCaM HRM (Carl Zeiss AG, DE) and provided with a ZEISS ZEN microscope software v2.3 (Carl Zeiss AG, DE). At least four different fields were analyzed for each sample and the number of nuclei determined with ImageJ version 1.52.n (University of Wisconsin, WI, USA; RRID:SCR_003070).
Di Leo L., Bodemeyer V., Bosisio F.M., Claps G., Carretta M., Rizza S., Faienza F., Frias A., Khan S., Bordi M., Pacheco M.P., Di Martino J., Bravo-Cordero J.J., Daniel C.J., Sears R.C., Donia M., Madsen D.H., Guldberg P., Filomeni G., Sauter T., Robert C., De Zio D, & Cecconi F. (2021). Loss of Ambra1 promotes melanoma growth and invasion. Nature Communications, 12, 2550.
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2

Quantifying Cell Death using TUNEL Assay

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To assess cell death, the TUNEL Assay Kit-BrdU-Red (Abcam, UK; cat# ab66110) was used following manufacturer’s instructions. Tissue sections were deparaffinized, rehydrated and treated with 20 µg/ml Proteinase K (ThermoFischer Scientific, MA, USA; cat# AM2548) for 5 min according to the kit’s protocol. Nuclear staining was performed for 10 min at room temperature with 1 µg/ml Hoechst 33342. Images were taken using a using a fluorescent AxIo Imager.A2 microscope (Carl Zeiss AG, DE) equipped with an AxioCaM HRM (Carl Zeiss AG, DE) and provided with a ZEISS ZEN microscope software v2.3 (Carl Zeiss AG, DE).
Di Leo L., Bodemeyer V., Bosisio F.M., Claps G., Carretta M., Rizza S., Faienza F., Frias A., Khan S., Bordi M., Pacheco M.P., Di Martino J., Bravo-Cordero J.J., Daniel C.J., Sears R.C., Donia M., Madsen D.H., Guldberg P., Filomeni G., Sauter T., Robert C., De Zio D, & Cecconi F. (2021). Loss of Ambra1 promotes melanoma growth and invasion. Nature Communications, 12, 2550.
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