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Anti human desmin d 33

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Anti-human desmin (D 33) is a laboratory reagent used for the detection of desmin, a protein found in muscle cells. It is commonly used in immunohistochemistry and other diagnostic techniques to identify the presence and distribution of desmin in tissue samples.

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Lab products found in correlation

Anti gapdh, by Merck Group (1 mentions) Vectashield mounting medium, by Vector Laboratories (1 mentions) Sqstm1 d 3, by Santa Cruz Biotechnology (1 mentions)

Close spelling variants of this product

Desmin (53 citations) Anti-desmin (9 citations) Anti-TM (1 citations)

2 protocols using anti human desmin d 33

1

Immunochemical Analysis of Muscle Cells

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The following monoclonal mouse antibodies were used in the study: anti-human desmin (D 33, Dako), anti-mouse M-cadherin (611100; BD Biosciencies), anti-GAPDH (glyceraldehyde 3-phosphodehydrogenase; clone 6C5; Merck Millipore), anti-human slow myosin heavy chain (MHCs; NOQ7.5.4D) and anti-rabbit sarcomeric α-actinin-1; EA-53 (both from Abcam. For immunocytochemistry studies, goat anti-mouse IgG conjugated with AlexaFluor-546 (A-21236; Life Technologies). DAPI applied in the Vectashield mounting medium (Vector Labs) was used to stain nuclei. For immunoblot, anti-mouse IgG (#AP308P) conjugated with horse radish peroxidase (HRP), from Millipore was used.
Fichna J.P., Karolczak J., Potulska-Chromik A., Miszta P., Berdynski M., Sikorska A., Filipek S., Redowicz M.J., Kaminska A, & Zekanowski C. (2014). Two Desmin Gene Mutations Associated with Myofibrillar Myopathies in Polish Families. PLoS ONE, 9(12), e115470.
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2

Muscle Tissue Histochemical Analysis

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Cryostat sections of fresh-frozen deltoid or quadriceps femoris muscle tissue were histochemically analyzed by standard techniques. For immunohistochemistry the following antibodies were used: SQSTM1 (D-3; Santa Cruz Biotechnology, Santa Cruz, CA; 1:1,000), Anti-Ubiquitin (P4D1-A11; Merck Millipore, Billerica, MA; 1:100), and Anti-Human Desmin (D33; Dako Cytomation, Carpinteria, CA; 1:50).
Malfatti E., Nilsson J., Hedberg-Oldfors C., Hernandez-Lain A., Michel F., Dominguez-Gonzalez C., Viennet G., Akman H.O., Kornblum C., den Bergh P.V., Romero N.B., Engel A.G., DiMauro S, & Oldfors A. (2014). A New Muscle Glycogen Storage Disease Associated with Glycogenin-1 Deficiency. Annals of neurology, 76(6), 891-898.
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