Tiliroside

Manufactured by Extrasynthese

Sourced in France

Tiliroside is a pure compound extracted from natural sources. It serves as a reference standard for analytical and research purposes.

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Lab products found in correlation

5 protocols using tiliroside

Krebs–Henseleit Buffer Preparation and Arterial Ring Viability Testing

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Chemicals used for Krebs–Henseleit buffer preparation and arterial ring viability testing in the pharmacological studies were purchased from Sigma-Aldrich^® (St. Louis, MO, USA). The following commercial standards were used in the ITA assays: quercetin 3-O-glucoside (Sigma, 17793-10MG-F), quercetin (G Buchs SG. K148-/49/2), p-coumaric acid (Sigma, C9008) and tiliroside (Extrasynthese, 1001 S, 20316-62-5). The selective prostacyclin IP receptor antagonist Ro 1138452 hydrochloride (4268) was purchased from Tocris (Bristol, UK).
Chemicals used for phytochemical characterization were purchased from Merck^® (Darmstadt, Germany) and correspond to the highest grade commercially available. The reference compounds used were: ellagic acid (Sigma, E2250-5G), p-coumaric acid (Sigma, C-9008, Lot: 22H0312), quercetin (G Buchs SG., Buchs, Switzerland, K148-/49/2), quercetin-3-O-glucoside (Sigma, 17793-10MG-F), tiliroside (Extrasynthese, Genay, France, 1001 S, Lot: 12080209), vitexin (Extrasynthese, 1232 S) and isovitexin (Extrasynthese, 1235 S).

Malheiros J., Simões D.M., Antunes P.E., Figueirinha A., Cotrim M.D, & Fonseca D.A. (2022). Vascular Effects of Polyphenols from Agrimonia eupatoria L. and Role of Isoquercitrin in Its Vasorelaxant Potential in Human Arteries. Pharmaceuticals, 15(5), 638.

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HPLC-based Phytochemical Quantification

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Methanol HPLC grade was obtained from Panreac Química (Barcelona, Spain). Acetonitrile HPLC grade, formic acid HPLC grade, SYBR safe DNA gel stain, and the primers MatK-1RKIM-f and MatK-3FKIM-r were from Thermo Fisher Scientific (Runcorn, Cheshire, UK). Agarose MB 250 was purchased from Biotools Biotechnological and Medical Laboratories SA (Madrid, Spain). Apigenin-7-glucoside, epigallocatechin, and tiliroside were acquired from Extrasynthese (Genay Cedex, France). Acetoxy acid was from Sigma-Aldrich (St. Louis, MO, USA), and valerenic acid was from Chromadex (Irvine, CA, USA).

Sánchez M., González-Burgos E., Divakar P.K, & Gómez-Serranillos M.P. (2020). DNA-Based Authentication and Metabolomics Analysis of Medicinal Plants Samples by DNA Barcoding and Ultra-High-Performance Liquid Chromatography/Triple Quadrupole Mass Spectrometry (UHPLC-MS). Plants, 9(11), 1601.

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Polyphenol Compound Identification

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Caffeic acid, chlorogenic acid, ellagic acid, gallic acid, salicylic acid, catechin, hyperoside, quercetin, isoquercetin, kaempferol 3-O-galactoside, myricetin and kaempferol were obtained from Fluka (Switzerland). Procyanidin B₁, procyanidin B₂, quercetin 3-O-glucuronide, quercetin 3-O-rhamnoside and tiliroside were obtained from Extrasynthèse (France). Protocatechuic acid, epicatechin and epigallocatechin were obtained from Sigma (Germany). Sanguiin H-6 was isolated according to the previously described procedure [9 (link)].

Krauze-Baranowska M., Głód D., Kula M., Majdan M., Hałasa R., Matkowski A., Kozłowska W, & Kawiak A. (2014). Chemical composition and biological activity of Rubus idaeus shoots – a traditional herbal remedy of Eastern Europe. BMC Complementary and Alternative Medicine, 14, 480.

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Calibration Curves for Polyphenol Analysis

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Standard calibration curves were prepared using the following standards: agrimoniin and bis-HHDP-glucose pedunculagin (obtained from the Institute of Food Technology and Analysis), quercetin-3-glucoside, tiliroside, kaempferol 3-glucoside, ellagic acid, quercetin 3-d-galactoside, quercetin, kaempferol, (−)-epicatechin-phloroglucinol adduct (after phloroglucinolysis of procyanidin B2 standard) (Extrasynthese, France), (−)-epicatechin and (+)-catechin (Sigma, Steinheim, Germany). Standard stock solutions were diluted to appropriate concentrations for the plotting of calibration curves. The linearity was obtained by plotting the peak areas versus the corresponding concentrations (mg/L) of each analyte. Table 7 shows the equations of the calibration curves and the correlation coefficients (R²) of the standards used.

Milala J., Piekarska-Radzik L., Sójka M., Klewicki R., Matysiak B, & Klewicka E. (2021). Rosa spp. Extracts as a Factor That Limits the Growth of Staphylococcus spp. Bacteria, a Food Contaminant. Molecules, 26(15), 4590.

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Flavonoid Monolayer Interactions Study

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Materials 4 DOPC with the purity of > 99% was purchased from Avanti Polar Lipids (USA). The 5 flavonoids quercetin dihydrate, rutin trihydrate (quercetin 3 β D rutinoside), naringenin, 6 hesperetin, (+) catechin hydrate and naringin were supplied by Sigma Aldrich (Germany). 7
Kaempferol and tiliroside were obtained from Extrasynthese (France). Phosphate buffered saline 8 (PBS) powder was used to prepare the pH 7.4 buffer solution, and was obtained from Sigma 9 Aldrich. For monolayer studies, one batch of PBS was dissolved in one liter of Milli Q water 10 (Millipore Inc., Ω = 18.2 MΩ.cm) to give 0.01 M phosphate, 0.138 mol dm 3 NaCl + 0.0027 mol 11 dm 3 KCl. Ethanol (absolute, AR, Merck) and dichloromethane (anhydrous, ≥ 99.8%, Sigma 12 Aldrich) were used as received without further purification. A flavonoid concentration of 10 13 µmol dm 3 was chosen for monolayer studies as it is in the range of physiological uptake 14 concentrations in body 20 and a dose dependent response was investigated in this range via RCV. 15

Sanver D., Murray B.S., Sadeghpour A., Rappolt M, & Nelson A.L. (2016). Experimental Modeling of Flavonoid-Biomembrane Interactions. Langmuir : the ACS journal of surfaces and colloids, 32(49).

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