The Cyan ADP flow cytometer (Beckman Coulter, Indianapolis IN) was used for all flow samples. Antibodies for these surface markers were used for flow cytometry: CD3 (145-2C11, BD Bioscience Franklin Lakes, NJ, USA), CD4 (RM4-5, BD Bioscience), anti-CEA CAR (Wi2, Immunomedics Morris Plains, NJ, USA), CD11b (M1/17, BD Bioscience), Ly6C (AL-21, BD Bioscience), Ly6G (1AB, BD Bioscience), PD-L1 (MIH5, BD Bioscience), CD62L (MEL-14, BD Bioscience), CCR7 (4B12, BD Bioscience), CD44 (IM7, BD Bioscience). Intracellular FoxP3 staining was performed with Mouse FoxP3 Permeabilization Kit (BD Bioscience). Single stain and isotype controls were used for each experiment. Analysis of acquired flow samples was performed with FlowJo software (Tree Star Inc., Ashland OR).
Mouse foxp3 permeabilization kit
Manufactured by BD
The Mouse FoxP3 Permeabilization Kit is a laboratory tool designed for the detection and analysis of the transcription factor FoxP3 in mouse samples. It provides the necessary reagents and protocols to permeabilize cells, enabling the intracellular staining and flow cytometric analysis of FoxP3 expression.
Automatically generated - may contain errors
Lab products found in correlation
Cd3 145 2c11, by BD (2 mentions)
Cd4 rm4 5, by BD (2 mentions)
Cd62l mel 14, by BD (2 mentions)
Cd11b m1 17, by BD (2 mentions)
Ly6g 1ab, by BD (2 mentions)
Pd l1 mih5, by BD (2 mentions)
Ccr7 4b12, by BD (2 mentions)
Cd44 im7, by BD (2 mentions)
Ly6c al 21, by BD (2 mentions)
Cyan adp flow cytometer, by Beckman Coulter (2 mentions)
2 protocols using mouse foxp3 permeabilization kit
1
Comprehensive Multiparameter Flow Cytometry
2
Comprehensive Multiparameter Flow Cytometry
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The Cyan ADP flow cytometer (Beckman Coulter, Indianapolis IN) was used for all flow samples. Antibodies for these surface markers were used for flow cytometry: CD3 (145-2C11, BD Bioscience Franklin Lakes, NJ, USA), CD4 (RM4-5, BD Bioscience), anti-CEA CAR (Wi2, Immunomedics Morris Plains, NJ, USA), CD11b (M1/17, BD Bioscience), Ly6C (AL-21, BD Bioscience), Ly6G (1AB, BD Bioscience), PD-L1 (MIH5, BD Bioscience), CD62L (MEL-14, BD Bioscience), CCR7 (4B12, BD Bioscience), CD44 (IM7, BD Bioscience). Intracellular FoxP3 staining was performed with Mouse FoxP3 Permeabilization Kit (BD Bioscience). Single stain and isotype controls were used for each experiment. Analysis of acquired flow samples was performed with FlowJo software (Tree Star Inc., Ashland OR).
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