8 anilinonaphthalene 1 sulfonic acid

Manufactured by Merck Group

Sourced in United States

8-anilinonaphthalene-1-sulfonic acid is a fluorescent dye compound used in various laboratory applications. It serves as a probe for the investigation of protein structure and dynamics.

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Close spelling variants of this product

8-anilinonaphthalene-1-sulfonic acid (ANS) (11 citations) 1-anilinonaphthalene 8-sulfonic acid (1,8-ANS) (2 citations) Anilinonaphthalene-8-sulfonic acid (2 citations)

11 protocols using 8 anilinonaphthalene 1 sulfonic acid

Protein Characterization via Sulfhydryl Assays

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Lysozyme from chicken egg white (about 100,000 U/mg), α-chymotrypsinogen A from a bovine pancreas, ribonuclease A from a bovine pancreas (Type XII-A, 75–125 Kunitz units/mg protein), l-cysteine, cysteamine, l-glutathione, oxidized glutathione, N-acetyl-l-cysteine, cysteinylglycine, l-cysteine ethyl ester, 1-chloro-2,4-dinitrobenzene, 5,5′-dithiobis(2-nitrobenzoic acid), 4-chloro-7-nitrobenzofurazane, dithiotreitol, ethylendiamminotetreaacetic acid, urea, 8-anilinonaphthalene-1-sulfonic acid and all of the other reagents were purchased from Sigma-Aldrich (St. Louis, MO, USA).

Gambardella G., Cattani G., Bocedi A, & Ricci G. (2020). New Factors Enhancing the Reactivity of Cysteines in Molten Globule-Like Structures. International Journal of Molecular Sciences, 21(18), 6949.

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Immunoglobulin G Isolation from Human Serum

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Methylglyoxal (MGO), Protein-A-agarose pre-packed affinity column, Congo red (CR), Thioflavin T (ThT), 8-anilinonaphthalene-1-sulfonic acid (ANS), sodium dodecyl sulphate, dialysis tubing, 2,4,6-trinitrobenzene sulphonic acid (TNBS) and standard molecular weight marker were purchased from Sigma Chemical Company (St. Louis, MO, USA). D-glucose was purchased from Qualigens, India. Nitroblue tetrazolium (NBT) dye, 2,4-dinitrophenylhydrazine (DNPH) and silver nitrate were obtained from SRL, India. Acrylamide, bisAcrylamide, ammonium persulphate and N,N,N’,N’-tetramethylethylene- diamine (TEMED) were purchased from Bio-Rad Laboratories, USA. All other reagents were of highest analytical grade available. The study protocol was approved by the Institutional Ethics Committee (IEC), Faculty of Medicine, Aligarh Muslim University, India. Furthermore, the blood sample was voluntarily donated by MAK and the same was used for IgG isolation. This was reported to the IEC.

Khan M.A., Arif Z., Khan M.A., M.o.i.n.u.d.d.i.n, & Alam K. (2018). Methylglyoxal produces more changes in biochemical and biophysical properties of human IgG under high glucose compared to normal glucose level. PLoS ONE, 13(1), e0191014.

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Synthesis and Characterization of PCB Metabolites

Cited 1 time

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All PCBs and PCB metabolites (4-chlorobiphenyl (PCB 3), 3,3′-dichlorobiphenyl (PCB 11), 4′-chloro-biphenyl-3-ol (3′-OHPCB 3), 4′-chloro-biphenyl-4-ol (4′-OHPCB 3), 3,3′-dichloro-biphenyl-4-ol (4-OHPCB 11), 3,3′,4′,5-tetrachloro-biphenyl-4-ol (4′-OHPCB 79) and ammonium salts of 4-chloro-3′-sulfooxy-biphenyl (3′-PCB 3 sulfate), 4-chloro-4′-sulfooxy-biphenyl (4′-PCB 3 sulfate), 3,3′-dichloro-4-sulfooxy-biphenyl (4-PCB 11 sulfate), 2,4′-dichloro-4-sulfooxy-biphenyl (4-PCB 8 sulfate), 2,3′,5-trichloro-4′-sulfooxy-biphenyl (4′-PCB 26 sulfate) and 2,3′,4′-trichloro-4-sulfooxy-biphenyl (4-PCB 33 sulfate)) used in this study were provided by the Synthesis Core of the University of Iowa Superfund Research Program and synthesized and characterized as described elsewhere (Figure 2).^{31 (link), 32 (link)} PCB sulfates were synthesized as the ammonium salts.^{32 (link)} Flufenamic acid, 8-anilinonaphthalene-1-sulfonic acid (ANS), and transthyretin purified from human plasma (> 95%) were all acquired from Sigma Aldrich (St. Louis, MO). The purity of TTR was routinely confirmed by SDS-PAGE.

Grimm F.A., Lehmler H.J., He X., Robertson L.W, & Duffel M.W. (2015). Modulating Inhibitors of Transthyretin Fibrillogenesis via Sulfation: Polychlorinated Biphenyl Sulfates as Models. Chemico-biological interactions, 228, 1-8.

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Protein Solubility Characterization with Fluorescent Probes

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The protein bovine serum albumin (BSA, molecular weight of 66 kDa, 583 residues) in the form of lyophilized powder, the fluorophore 8-anilinonaphthalene-1-sulfonic acid (ANS), the salts MgCl₂, Mg(ClO₄)₂ and MgSO₄ were all purchased from Sigma Aldrich Chemicals (Taufkirchen, Germany). All the sample solutions were prepared in the pressure stable 10 mM Tris-HCl buffer, at the pH of 7.4. Deionized water was used for all buffer and sample preparations.

Jahmidi-Azizi N., Oliva R., Gault S., Cockell C.S, & Winter R. (2021). The Effects of Temperature and Pressure on Protein-Ligand Binding in the Presence of Mars-Relevant Salts. Biology, 10(7), 687.

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Biophysical Characterization of BSA Protein

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BSA (fatty acid depleted, catalogue no. A7638, 99+ % of purity), sodium phosphate monobasic, sodium phosphate dibasic and sodium chloride were purchased from Sigma—Aldrich and used without further purification. The fluorescent probes thioflavin T (ThT) and 8-anilinonaphthalene-1-sulfonic acid (ANS) were obtained from Sigma—Aldrich. All solutions for the experiments were prepared using deionized water obtained with Easy-Pure II RF system (Barnstead, USA). BSA samples were prepared by dissolving solid BSA in 0.1 M phosphate buffer solutions at pH 7.0. All experiments were performed with freshly prepared solutions of BSA. BSA concentration was determined spectrophotometrically at 280 nm using the absorption coefficient

A_{cm}^{1 %}

of 6.58 [39 (link)].

Borzova V.A., Markossian K.A., Chebotareva N.A., Kleymenov S.Y., Poliansky N.B., Muranov K.O., Stein-Margolina V.A., Shubin V.V., Markov D.I, & Kurganov B.I. (2016). Kinetics of Thermal Denaturation and Aggregation of Bovine Serum Albumin. PLoS ONE, 11(4), e0153495.

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Carbonic Anhydrase Inhibition Assay

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Carbonic anhydrase (CA, from bovine erythrocytes), Bovine serum albumin (BSA, from bovine), Transthyretin (TTR, from human plasma), and other chemicals were purchased from Sigma-Aldrich chemical Co. S-allyl l-cysteine (SAC) was purchased from Cayman Chemicals. Methyl Glyoxal (MGO), Glyoxal (GO), Fructose, 8-Anilinonaphthalene-1-sulfonic acid (ANS), Dinitrophenyl hydrazine (DNPH), p-Nitrophenyl acetate (p-NPA), 2-Thiobarbituric acid (TBA) and N-acetyl l-cysteine (NAC) were also obtained from Sigma Chemical Co.

Bhattacharya R., Saini S., Ghosh S., Roy P., Ali N., Parvez M.K., Al-Dosari M.S., Mishra A.K, & Singh L.R. (2023). Organosulfurs, S-allyl cysteine and N-acetyl cysteine sequester di-carbonyls and reduces carbonyl stress in HT22 cells. Scientific Reports, 13, 13071.

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Lipid Membrane Biophysical Characterization

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Genscript USA Inc., with >76.9% purity. Sodium dodecyl sulfate (SDS), trimethylamine Noxide (TMAO), 2,2,2-trifluoroethanol (TFE), thioflavin T (ThT), and 8-anilinonaphthalene-1sulfonic acid (ANS) were purchased from Sigma-Aldrich (St. Louis, USA). 1,2-dioleoyl-snglycero-3-phospho-L-serine (DOPS) and 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC))
were purchased from Avanti Polar Lipids (Alabaster, Alabama, U.S.A.).

Gadhave K., Kumar A., Kumar P., Kapuganti S.K., Garg N., Vendruscolo M., & Giri R. (2020). Environmental Dependence of the Structure of the C-terminal Domain of the SARS-CoV-2 Envelope Protein.

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Hydrophobic Binding Assessment of Der p 2

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Staining Der p 2 with 1-anilinonaphthalene 8-sulfonic acid (ANS; Sigma-Aldrich) to measure the hydrophobic binding capacity was conducted as before.11 (link)16 (link) Briefly, ANS was dissolved in methanol at a concentration predetermined from an absorbance value at 372 nm using a molar extinction coefficient of 8,000 M⁻¹.11 (link)16 (link) The mixture of 200 µM ANS and 3.5 µM D20110 or S47W was incubated for 10 minutes at 25°C before determining the emission spectra of ANS on a Jasco FP-6300 fluorometer. ANS was excited at 390 nm with a 5-nm slit width, and the emission spectra were measured from 400–600 nm with a 5-nm slit width.

Kulwanich B., Thanyaratsrisakul S., Jirapongsananuruk O., Hales B.J., Thomas W.R, & Piboonpocanun S. (2018). Effects of Ser47-Point Mutation on Conformation Structure and Allergenicity of the Allergen of Der p 2, a Major House Dust Mite Allergen. Allergy, Asthma & Immunology Research, 11(1), 129-142.

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Histone H1 Modification and Detection

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Histone H1, 2,4-dinitrophenyl hydrazine (DNPH), 1-anilinonaphthalene-8-sulfonic acid (ANS), sodium dodecyl sulfate (SDS), methylglyoxal, aminoguanidine hydrochloride, diethylene triamine penta-acetic acid (DTPA), sodium azide, ethidium bromide, protein A-agarose (2.5ml pre-packed column), agarose, sodium azide, Tween-20, dialysis tubings, anti-human and anti-rabbit IgG, alkaline phosphatase conjugate, para-nitrophenyl phosphate, Freund’s complete and incomplete adjuvants were purchased from Sigma Chemical Company, St. Louis, MO, USA. Acrylamide, bisAcrylamide, ammonium persulfate (APS) and N,N,N′,N′- tetramethylethylenediamine (TEMED) were from Bio-Rad Laboratories, U.S.A. Sodium hydroxide, Ethylenediaminetetraacetic acid (disodium salt), methanol, glacial acetic acid, iso-propanol, sodium chloride, sodium carbonate, sodium nitrite, silver nitrate, xylene, sodium hydroxide, formaldehyde, sodium bicarbonate, ethanol, ammonium sulphate and ammonium persulphate were obtained from Qualigens, India. Polystyrene microtitre flat bottom ELISA plates and modules were purchased from NUNC, Denmark. All other chemicals/reagents were of the highest analytical grade available.

Mir A.R., Uddin M., Khan F., Alam K, & Ali A. (2015). Dicarbonyl Induced Structural Perturbations Make Histone H1 Highly Immunogenic and Generate an Auto-Immune Response in Cancer. PLoS ONE, 10(8), e0136197.

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Detergent Screening for Protein Purification

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The following detergents were used: 1-palmitoyl-2-hydroxy-sn-glycero-3-phospho-(1’-rac-glycerol) (LPPG; Avanti Polar Lipids, Inc.); octyl β-D-glucopyranoside (OG; Sigma-Aldrich); n-dodecylphosphocholine (DPC; Affymetrix); empigen (Sigma-Aldrich); n-dodecyl β-D-maltopyranoside (DDM; Sigma-Aldrich); 1-myristoyl-2-hydroxy-sn-glycero-3-phospho-(1’-rac-glycerol) (LMPG; Affymetrix); 1-oleoyl-2-hydroxy-sn-glycero-3-phospho-(1’-rac-glycerol) (LOPG; Avanti Polar Lipids, Inc.). His60 Ni Superflow resin was purchased from Clontech. Protease inhibitor cocktail was purchased from Fermentas and RNAse was from Thermo Scientific. DNAse, glutaraldehyde, and 1-anilinonaphthalene-8-sulfonic acid (ANS) were purchased from Sigma-Aldrich. All other reagents were of analytical grade.

Chadwick A.C., Jensen D.R., Peterson F.C., Volkman B.F, & Sahoo D. (2014). Expression, purification and reconstitution of the C-terminal transmembrane domain of scavenger receptor BI into detergent micelles for NMR analysis. Protein expression and purification, 0, 35-42.

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