Jmp version 12 for windows

Comparisons between two groups were performed using the t-test. Differences in survival between groups were analyzed by the log-rank test. All statistical analyses were conducted using the JMP for Windows version 12.0 software package (SAS Institute Inc., Cary, NC, USA).

A ROC curve was used to determine the cutoff values of continuous variables such as the numbers of CD4+, CD8+ or Foxp3+ T lymphocytes or the H-scores of EMT-related proteins or PD-L1 (Supplementary Figure 1). The values of the percentages of tumor cells with recognized staining alteration as a continuous variable, and survival (alive or dead at the median follow-up time) as a binary variable were subjected to ROC analysis, as previously described [30 (link)].
The correlation of each factor such as the tumor infiltration of CD4+, CD8+ or Fop3+ lymphocytes, PD-L1 expression, EMT-related protein expression and clinicopathological factors was analyzed using the Pearson Chi-squared method. Survival was estimated with the Kaplan-Meier method, and survival estimates were compared using the log-rank test. Overall survival was calculated from the date of surgery to the date of death from any cause or last contact/follow-up. Multivariate analysis was conducted using Cox proportional hazards regression modeling. Baseline variables with P < 0.05 in univariate analysis were included in multivariate models. The threshold for significance was P < 0.05. All statistical analyses were conducted using the JMP for Windows version 12.0 software package (SAS Institute, Cary, NC).

Data were analyzed as described using GraphPad Prism version 6 for Windows (GraphPad Software, La Jolla, CA USA) or JMP version 12 for Windows (SAS Institute, Inc., Cary, NC USA). For all statistics, p<0.05 was used for statistical significance. For clinicopathologic variables, normally distributed variables were compared with 2-tailed t-test and non-normally distributed variables with the Mann-Whitney U test. Fisher's Exact test was used for mean comparisons between >2 groups. Chi-Square test was used to compare proportions.

This study was reviewed and approved by the Human Ethical Committee of the University of Teikyo Hospital (Trial registration number: 20-094). The de-identified medical records of 323 female patients who underwent TLH from June 1, 2015 to December 31, 2019 were reviewed retrospectively. In these cases, bilateral salpingectomy (BS) or BSO was performed during TLH. This study also included 25 cases with concomitant PLA and 19 cases with concomitant LC, including 2 bilateral LCs and 17 unilateral LCs. Among the 323 cases, we extracted representative patients’ characteristics and surgical complications as described below. Statistical analyses were performed using JMP version 12 for Windows (SAS Institute, Inc., Tokyo, Japan) to determine the correlations between patient characteristics and surgical complications. The odds ratios (ORs) and 95% confidence intervals (CIs) were estimated to determine the strengths of the correlations. P < 0.05 was considered statistically significant.