Lenti dscontrol

Manufactured by GenePharma

Sourced in China

Lenti-dsControl is a laboratory product designed for the generation of lentiviral particles. It provides a control vector for monitoring lentiviral transduction efficiency in cell lines.

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Lab products found in correlation

Mirna mimic, by RiboBio (1 mentions) Rna duplexes, by RiboBio (1 mentions) Si β catenin, by RiboBio (1 mentions) Mir 3619 5p mimic, by RiboBio (1 mentions) Mir 3619 5p inhibitor, by RiboBio (1 mentions) Si p21, by RiboBio (1 mentions) Si control, by RiboBio (1 mentions)

3 protocols using lenti dscontrol

miRNA Mimics and Knockdown Protocol

Cited 1 time

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The miRNA mimics and 5′- or 3′- biotin linked miRNA mimics were synthesized by RiboBio Co., Ltd. (Guangzhou, China). A short interfering RNA (siCDH1) was used to knockout E-cadherin expression and a dsControl which lacks of significant homology to all known human sequences was synthesized and used as a negative control [12 (link), 44 (link)]. Besides, Lenti-miR-373 and Lenti-dsControl were structured by GenePharma (Shanghai, China). The sequences of the custom dsRNAs and miRNA are listed in Supplementary Table 1.

Zhang Q., Wang C., Miao S., Li C., Chen Z, & Li F. (2017). Enhancing E-cadherin expression via promoter-targeted miR-373 suppresses bladder cancer cells growth and metastasis. Oncotarget, 8(55), 93969-93983.

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Synthesis of RNA Duplexes for Gene Silencing

Cited 1 time

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All the RNA duplexes used in present study which possesses 2-nucleotide 3’ overhangs were chemically synthesized by RiboBio Co., Ltd. (Guangzhou, China). A small interfering RNA (siP53) was used to silence p53 expression and a dsControl which lacks significant homology to all known human sequences was used as a negative control [10 (link), 13 (link)]. Lenti-dsP53-285 and Lenti-dsControl were purchased from GenePharma (Shanghai, China). The sequences of all the custom dsRNAs are listed in Additional file 1: Table S1.

Wang C., Ge Q., Zhang Q., Chen Z., Hu J., Li F, & Ye Z. (2016). Targeted p53 activation by saRNA suppresses human bladder cancer cells growth and metastasis. Journal of Experimental & Clinical Cancer Research : CR, 35, 53.

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miR-3619-5p Manipulation Protocols

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The miR-3619-5p mimic, 5′- or 3′- biotin covalently  linked miR-3619 mimic, miR-3619-5p inhibitor, miR-3619-5p mutation as well as interfering RNAs (si-p21, si-β-catenin, si-CDK2 and si-Control) were chemically synthesized by RiboBio Co., Ltd. (Guangzhou, China). Lenti-dsControl and Lenti-miR-3619 were synthesized by GenePharma (Shanghai, China). The sequences of the dsRNA and miRNA were listed in Supplementary Table 1.

Zhang Q., Miao S., Han X., Li C., Zhang M., Cui K., Xiong T., Chen Z., Wang C, & Xu H. (2018). MicroRNA-3619-5p suppresses bladder carcinoma progression by directly targeting β-catenin and CDK2 and activating p21. Cell Death & Disease, 9(10), 960.

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