Dropsens drp 110swcnt

Genosensors were assembled on SWCNT-SPEs and GC-SPEs purchased by Metrohm Italiana (DropSens DRP-110SWCNT and DRP-110, respectively) (Origgio, Italy).
All electrochemical measurements were performed with a PGSTAT-204 potentiostat/galvanostat produced by Metrohm Italiana, equipped with NOVA 2.1.3 Advanced Electrochemical Software and connected to DropSens DRP-DSC plug.
All the measurements carried out to assess the best experimental conditions in terms of proper concentration of CP and SP, as well as the signal values for construction of calibration curves, were replicated three times, carrying out independent measurements on different SPEs. Mean values and standard deviation are shown in all figures.
Method validation was performed by assessing the linearity range and calculating Limit of Detection (LOD) and Limit of Quantification (LOQ) according to “Eurachem Guides” (http://www.eurachem.org/index.php/ publications/guides/mv). Precision was evaluated in terms of intermediate precision by carrying out three independent assays for each target DNA concentration level.

Sodium chloride (NaCl), potassium chloride (KCl), potassium dihydrogen phosphate (KH₂PO₄), disodium hydrogen phosphate (Na₂HPO₄), Trizma^® Base, sodium hydroxide (NaOH), hydrochloric acid (37% w/v) (HCl), magnesium chloride (MgCl₂), potassium ferrocyanide (K₄[Fe(CN)₆]), N-(3-dimethyl)-N′-ethylcarbodiimide hydrochloride (EDC), N-Hydroxysuccinimide (NHS), 4-morpholineethanesulfonic acid monohydrate (MES), Tween^® 20 and plasma from bovine, were purchased from Merck (Milan, Italy). Sars-CoV-2 Nucleocapsid His T protein, goat anti-human IgG conjugated with alkaline phosphatase (Anti-Hu-IgG-AP) and goat anti-human IgM conjugated with alkaline phosphatase (Anti-Hu-IgM-AP) were purchased from Thermo Fisher Scientific (Milan, Italy). Sars-CoV-2 Nucleocapsid antibody IgG (anti-N-IgG) and IgM (anti-N-IgM), Sars-CoV-2 Spike protein S1, SarsCoV-2 Spike S1 IgG (anti-S1-IgG) and IgM (anti-S1-IgM) antibodies, Sars-CoV-2 Spike protein RBD mFc Tag, MonoRab™ AntiMouse IgG (RAM) were purchased from GenScript (Leiden, The Netherlands). Hydroquinone diphosphate (HQDP), SWCNT-SPEs (DropSens DRP-110SWCNT), GNP-SPEs (DropSens DRP-110GNP) and SWCNT/GNP-SPEs (DropSens DRP-110CNT-GNP) were purchased from Metrohm Italiana s.r.l. (Origgio, Italy).