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Phospho fgf receptor tyr653 654

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The Phospho-FGF Receptor (Tyr653/654) product is an antibody that detects the phosphorylation of the fibroblast growth factor (FGF) receptor at tyrosine residues 653 and 654. This antibody is used to study the activation of the FGF receptor signaling pathway.

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Lab products found in correlation

Total mapk, by Cell Signaling Technology (2 mentions) P44 42 mapk erk1 2, by Cell Signaling Technology (2 mentions) Total mek1 2, by Cell Signaling Technology (2 mentions) Phospho mek1 2, by Cell Signaling Technology (2 mentions) Pi3 kinase p85 19h8, by Cell Signaling Technology (2 mentions) Fgf receptor 2 d4l2v, by Cell Signaling Technology (2 mentions) Phospho pi3 kinase p85 tyr458 p55 tyr199, by Cell Signaling Technology (2 mentions) Total akt, by Cell Signaling Technology (2 mentions) Phospho akt ser473, by Cell Signaling Technology (2 mentions) β actin, by Cell Signaling Technology (2 mentions) Pfrs2, by Cell Signaling Technology (1 mentions) Anti frs2, by Abcam (1 mentions) Gapdh, by Santa Cruz Biotechnology (1 mentions) Phospho p44 42 mapk erk1 2 thr202 tyr204, by Cell Signaling Technology (1 mentions) Sch772984, by Selleck Chemicals (1 mentions) Phospho plc γ1 tyr783, by Cell Signaling Technology (1 mentions) Phospho frs2α tyr 196, by Cell Signaling Technology (1 mentions)

Spelling variants of this product

Phospho-FGF Receptor (2 citations)

3 protocols using phospho fgf receptor tyr653 654

1

Western Blot Antibody Profiles

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Western blot assays were performed using established protocols with the following antibodies: phospho-Akt (Ser473) 1:1000 (Cell Signaling 9271), Total Akt 1:1000 (Cell Signaling 9272), phospho-MEK1/2 1:5000 (Cell Signaling 9154), Total MEK1/2 1:5000 (Cell Signaling 9122), phospho-FGF Receptor (Tyr653/654) 1:500 (Cell Signaling 3471), FGF Receptor 2 (D4L2V) 1:500 (Cell Signaling 23328), p44/42 MAPK (Erk1/2) 1:5000 (Cell Signaling 9101), Total MAPK 1:5000 (Cell Signaling 9102), pFRS2 1:1000 (Cell Signaling 3864), anti-FRS2 (abcam ab10425), phospho-PI3 Kinase p85 (Tyr458)/p55 (Tyr199) 1:1000 (Cell Signaling 4228), PI3 Kinase p85 (19H8) 1:000 (Cell Signaling 4257), β-actin 1:10000 (Cell Signaling 4967) and GAPDH 1:10000 (Santa Cruz sc-25778).
Krook M.A., Barker H., Chen H.Z., Reeser J.W., Wing M.R., Martin D., Smith A.M., Dao T., Bonneville R., Samorodnitsky E., Miya J., Freud A.G., Monk J.P., Clinton S.K, & Roychowdhury S. (2019). Characterization of a KLK2-FGFR2 fusion gene in two cases of metastatic prostate cancer. Prostate cancer and prostatic diseases, 22(4), 624-632.
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2

FGF2-Mediated ERK1/2 Activation Assay

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Transduced mammary organoids were serum starved for 12 hours and left unstimulated or stimulated with 10 ng/ml FGF2 for the indicated times. ERK1/2 inhibitor SCH772984 was obtained from Selleckchem (Newmarket, Suffolk, UK; #S7101). Organoids were released from Matrigel using non-enzymatic cell recovery solution (BD Biosciences) and then lysed in Laemmli buffer (2% SDS, 10% glycerol, 1.25% beta-mercaptoethanol, 0.002% bromphenol blue, 0.0625 M Tris pH 6.8). Following SDS-PAGE, protein extracts were transferred to a PVDF membrane and probed with antibodies to p44/42 MAPK (ERK1/2) (Cell Signalling Technologies, Leiden, The Netherlands, Antibody #9102), phospho-p44/42 MAPK (ERK1/2) (Thr202/Tyr204) (Cell Signalling Technologies, Antibody #9101), phospho-FGF Receptor (Tyr653/654) (Cell Signalling Technologies, Antibody #3471), FGFR1 (9740, Cell Signaling, Technology, Rabbit monoclonal, clone D8E4), FGFR2 (H00002263-M01, Abnova, Mouse monoclonal, clone 1G3), FGFR3 (PA5-34574, Rabbit polyclonal, ThermoFisher Scientific), FGFR4 (HPA028251, Rabbit polyclonal, Sigma Aldrich) or anti-tubulin (clone BM1A), Sigma, antibody #T6199). After incubation with peroxidase-conjugated secondary antibodies, immunocomplexes were detected using Enhanced Chemiluminescent (ECL) reagents. Densitometric analysis was performed using Image J software.
Soady K.J., Tornillo G., Kendrick H., Meniel V., Olijnyk-Dallis D., Morris J.S., Stein T., Gusterson B.A., Isacke C.M, & Smalley M.J. (2017). Receptor protein tyrosine phosphatase PTPRB negatively regulates FGF2-dependent branching morphogenesis. Development (Cambridge, England), 144(20), 3777-3788.
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3

Signaling Pathway Protein Analysis

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Western blot assays were carried out using established protocols and probed with the following antibodies: phospho-Akt (Ser473) 1:1000 (Cell Signaling 9271), Total Akt 1:1000 (Cell Signaling 9272), phospho-MEK1/2 1:5000 (Cell Signaling 9154), Total MEK1/2 1:5000 (Cell Signaling 9122), p44/42 MAPK (Erk1/2) 1:5000 (Cell Signaling 9101), Total MAPK 1:5000 (Cell Signaling 9102), phospho-FGF Receptor (Tyr653/654) 1:500 (Cell Signaling 3471), FGF Receptor 2 (D4L2V) 1:500 (Cell Signaling 23328), phospho-PLCγ1 (Tyr783) 1:1000 (Cell Signaling 14008), PLCγ1 (D9H10) 1:1000 (Cell Signaling 5690), phospho-FRS2-α (Tyr196) 1:1000 (Cell Signaling 3864), FRS2 1:1000 (abcam 10425), phospho-PI3 Kinase p85 (Tyr458)/p55 (Tyr199) 1:1000 (Cell Signaling 4228), PI3 Kinase p85 (19H8) 1:000 (Cell Signaling 4257), β-actin 1:10000 (Cell Signaling 4967).
Krook M.A., Bonneville R., Chen H.Z., Reeser J.W., Wing M.R., Martin D.M., Smith A.M., Dao T., Samorodnitsky E., Paruchuri A., Miya J., Baker K.R., Yu L., Timmers C., Dittmar K., Freud A.G., Allenby P, & Roychowdhury S. (2019). Tumor heterogeneity and acquired drug resistance in FGFR2-fusion-positive cholangiocarcinoma through rapid research autopsy. Cold Spring Harbor Molecular Case Studies, 5(4), a004002.
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