A PDMS microfluidics slide chip (Abnova) was coated with 1 mg/mL streptavidin (Agilent). The chip was then washed and coated with 0.4 mg/mL biotin-vimentin (MD Anderson) for 1 h. Next, 1.5 × 106 attIL12-PBMCs (labeled with Calcein Green AM [ThermoFisher]) and 1.5 × 106 control-PBMCs (labeled with CMTPX red cell tracker [ThermoFisher]) were mixed at a ratio of 1:1, loaded into a spiral chamber (Abnova), and passed through the slide chip at a flow rate of 1.8 mL per hour using the CytoQuest microfluidics pump (Abnova). The slide chip was then imaged on a fluorescent microscope (Keyence). Cells were counted using Keyence BZ-X700 analysis software.
Spiral chamber
Manufactured by Abnova
The Spiral Chamber is a lab equipment product designed to facilitate specific experimental procedures. It functions as a contained space to conduct controlled tests or observations. The core purpose of the Spiral Chamber is to provide a structured environment for research activities, without making claims about its intended use or application.
Automatically generated - may contain errors
3 protocols using spiral chamber
1
Microfluidic Assay for Immune Cell Binding
2
PDMS Microfluidics for T-Cell Interaction
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A PDMS microfluidics slide chip (Abnova) was coated with 1 mg/mL streptavidin (Agilent). The chip was then washed and coated with 4 mg/mL rmFGL2 (5257-FL, R&D Systems) conjugated with biotin for 1 h. Following this, 1.5 million T-Ctr cells (dyed green) and 1.5 million T-αFGL2 cells (dyed red) were loaded into a spiral chamber (Abnova) and passed through the slide chip at a flow rate of 1.8 mL per hour using the Cytoquest microfluidics pump (Abnova). The slide chip was then imaged on a fluorescent microscope (Keyence).
3
Vimentin-Specific T Cell Capture
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A polydimethylsiloxane microfluidics slide chip (Abnova) was coated with 1 mg/mL streptavidin (Agilent). The chip was then washed and coated with 0.4 mg/mL biotin-vimentin (MD Anderson) for 1 hour. Next, 1.5×106 attIL12-T cells (labeled with Calcein Green AM; ThermoFisher) and 1.5×106 control-T cells (labeled with CMTPX red cell tracker; ThermoFisher) were mixed at a ratio of 1:1, loaded into a spiral chamber (Abnova), and passed through the slide chip at a flow rate of 1.8 mL per hour using the Cytoquest microfluidics pump (Abnova). The slide chip was then imaged on a fluorescent microscope (Keyence). Cells were enumerated using Keyence BZ-X700 analysis software.
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