WI-38 cells were pretreated with 40 μM CORM-A1 for 6 h followed by the administration of 25 μg/ml for 96 h. During the process of cellular senescence, WI-38 cells were posttreated with 40 μM CORM-A1 for 6 h every two days. After a 4-day incubation, cultured supernatant was collected, and the concentrations of PAI-1 and several SASPs, including IL-6, TNF-α, and IL-1β, were measured by human PAI-1 (DuoSet ELISA DY9387-05, BD Biosciences), human IL-6 (Cusabio Biotech, Wuhan, China), human TNF-α (Cusabio Biotech, Wuhan, China), and human IL-1β (Cusabio Biotech, Wuhan, China) ELISA kit according to the manufacturer's instructions.
Human tnf α
Manufactured by Cusabio
Sourced in China
Human TNF-α is a recombinant protein. It is used as a standard or control in research applications involving the quantitative determination of human tumor necrosis factor alpha.
Automatically generated - may contain errors
Lab products found in correlation
Heat inactivated fetal bovine serum, by Thermo Fisher Scientific (1 mentions)
Trypsin, by Thermo Fisher Scientific (1 mentions)
Human umbilical vein endothelial cells (huvec), by ScienCell (1 mentions)
Endothelial cell medium (ecm), by ScienCell (1 mentions)
Penicillin streptomycin solution, by Merck Group (1 mentions)
Rpmi 1640, by ScienCell (1 mentions)
2 protocols using human tnf α
1
Senescence-Associated Secretory Phenotype Analysis
2
In Vitro and In Vivo Pharmacological Assays
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The 1H-NMR Bruker 500-MHz Ultrashield (Billerica, Massachusetts, USA), TECAN Multi-mode microplate reader Model Infinite 200 (Mannedorf, Switzerland), and Buchi Rotavapor Model R-210/215 (Flawil, Switzerland) were used. The tail flick analgesia meter was purchased from IITC Life Sciences, CA, USA. Methylthiazolyldiphenyl-tetrazolium bromide reagent (MTT), Salmonella abortus lipopolysachharide (LPS), dimethyl sulfoxide (DMSO), phosphate-buffered saline (PBS), suramine, and penicillin/streptomycin (PS) solution were purchased from Sigma-Aldrich, Germany. Human IL-1, human TNF-α, human nitric oxide (NO), human vascular endothelial growth factor (VEGF), rat IL-1, and rat TNF-α ELISA kits were purchased from Cusabio, China. RPMI 1640, human umbilical vein endothelial cells (HUVEC), and endothelial cell medium (ECM) supplied with endothelial cell growth supplements (ECGS) were obtained from ScienCell, USA. Trypsin and heat-inactivated fetal bovine serum were purchased from GIBCO, UK. Matrigel matrix (10 mg/ml) was obtained from SABiosciences, USA. All other chemicals used in this study were analytical-grade or better.
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