Log In Sign up
The largest database of trusted experimental protocols

Titan krios 300kv feg tem

Manufactured by Thermo Fisher Scientific
Visit Supplier

The Titan Krios 300kV FEG-TEM is a high-resolution transmission electron microscope. It features a 300kV field emission gun and is designed for advanced cryo-electron microscopy applications.

Automatically generated - may contain errors

Lab products found in correlation

Em gp plunge freezer, by Leica (2 mentions) Falcon 2 cmos, by Thermo Fisher Scientific (2 mentions)

2 protocols using titan krios 300kv feg tem

1

Cryo-EM Imaging of C. burnetii OM Vesicles

Check if the same lab product or an alternative is used in the 5 most similar protocols
Stationary phase C. burnetii samples were frozen on a glow discharged Quantifoil™ R 2/2 grid using a Leica EM GP plunge freezer (Leica Microsystems, Wetzlar, Germany) set to a relative humidity of 100%. Grids were mounted in an autogrid assembly and cryo 2D images collected using an FEI Titan Krios 300kV FEG-TEM and an FEI Falcon II CMOS direct electron detection camera. Low dose images of 13e/A2 were captured at 6 μm defocus with a nominal magnification of 29 K which corresponds to a pixel size of 2.87 Å using a 70 μm objective aperture. OM vesiculation was quantified by imaging ≥50 bacterial cells per strain and is expressed as the percentage of bacteria with vesicles clearly attached to the bacterial OM.
Sandoz K.M., Moore R.A., Beare P.A., Patel A.V., Smith R.E., Bern M., Hwang H., Cooper C.J., Priola S.A., Parks J.M., Gumbart J.C., Mesnage S, & Heinzen R.A. (2020). β-Barrel Proteins Tether the Outer Membrane in Many Gram-Negative Bacteria. Nature microbiology, 6(1), 19-26.
+ Open protocol
+ Expand
2

Cryo-EM Imaging of C. burnetii OM Vesicles

Check if the same lab product or an alternative is used in the 5 most similar protocols
Stationary phase C. burnetii samples were frozen on a glow discharged Quantifoil™ R 2/2 grid using a Leica EM GP plunge freezer (Leica Microsystems, Wetzlar, Germany) set to a relative humidity of 100%. Grids were mounted in an autogrid assembly and cryo 2D images collected using an FEI Titan Krios 300kV FEG-TEM and an FEI Falcon II CMOS direct electron detection camera. Low dose images of 13e/A2 were captured at 6 μm defocus with a nominal magnification of 29 K which corresponds to a pixel size of 2.87 Å using a 70 μm objective aperture. OM vesiculation was quantified by imaging ≥50 bacterial cells per strain and is expressed as the percentage of bacteria with vesicles clearly attached to the bacterial OM.
Sandoz K.M., Moore R.A., Beare P.A., Patel A.V., Smith R.E., Bern M., Hwang H., Cooper C.J., Priola S.A., Parks J.M., Gumbart J.C., Mesnage S, & Heinzen R.A. (2020). β-Barrel Proteins Tether the Outer Membrane in Many Gram-Negative Bacteria. Nature microbiology, 6(1), 19-26.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!