Serum creatinine (SCr) (lot#: C011-1-1), blood urea nitrogen (BUN) (#C013-1-1), serum uric acid (SUA) (#C012-1-1), triglyceride (TG) (#A110-1-1), total cholesterol (TC) (#A111-1-1), and urine protein (#C035-2-1) assay kits were from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). Anti-α-smooth muscle actin (αSMA) antibody (#bs-0189R) was purchased from Beijing Biosynthesis Biotechnology Co.,Ltd. (Beijing, China). Anti-Rac1(#ab33186), anti-GTP-Rac1 (#ab33186), anti-p-PAK1 (#ab75599), anti-p38MAPK (#ab195049), anti-p-p38MAPK (#ab47363), anti-β-catenin (#ab32572), anti-Podocin (#ab50339), and anti-nephrin (#ab216341) antibodies were from Abcam (Cambridge, United Kingdom). Anti-β-actin (#66009-1-Ig), anti-fibroblast-specific protein-1 (FSP-1) (#16105-1-AP), anti-PAK1 (#21401-1-AP), anti-snail (#13099-1-AP), HRP goat anti-mouse IgG (#SA00001-1), and HRP goat anti-rabbit IgG (#SA00001-2) antibodies were from Proteintech (Chicago, United States). Trizol (#15596026) was from Thermo Scientific (MA, United States). A Reverse Transcription kit (#CW2569) was purchased from CWBio Co., Ltd. (Beijing, China). STZ (#WXBC8740V) was from Sigma-Aldrich Co. Ltd. (MO, United States). Metformin (#A181224) from Zhejian Yatai Pharmaceutical Co., Ltd. (Shaoxing, China).
Anti p p38 mapk
Manufactured by Abcam
Sourced in United Kingdom
Anti-p-p38 MAPK is a primary antibody that specifically recognizes the phosphorylated form of p38 mitogen-activated protein kinase (MAPK). p38 MAPK is a family of serine/threonine protein kinases that play a crucial role in various cellular processes, including stress response, inflammation, and cell differentiation.
Automatically generated - may contain errors
Lab products found in correlation
Anti p38 mapk, by Abcam (4 mentions)
Trizol, by Thermo Fisher Scientific (2 mentions)
Anti podocin, by Abcam (1 mentions)
Anti p pak1, by Abcam (1 mentions)
Anti nephrin, by Abcam (1 mentions)
Anti β catenin, by Abcam (1 mentions)
Hrp goat anti rabbit igg, by Proteintech (1 mentions)
Anti stat1, by Cell Signaling Technology (1 mentions)
Anti gadph, by Cell Signaling Technology (1 mentions)
Anti akt, by Cell Signaling Technology (1 mentions)
Anti p stat1, by Cell Signaling Technology (1 mentions)
Anti p stat3, by Cell Signaling Technology (1 mentions)
Anti p akt, by Cell Signaling Technology (1 mentions)
Anti arg 1, by Abcam (1 mentions)
Anti stat6, by Cell Signaling Technology (1 mentions)
Anti p jnk, by Cell Signaling Technology (1 mentions)
Anti p stat6, by Cell Signaling Technology (1 mentions)
Anti stat3, by Cell Signaling Technology (1 mentions)
Skimmed milk powder, by BD (1 mentions)
Anti il 1β, by Abcam (1 mentions)
5 protocols using anti p p38 mapk
1
Diabetic Kidney Disease Biomarker Analysis
2
Immunoblotting Analysis of Inflammatory Markers
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All cell lysates were separated by 10% or 12% SDS-polyacrylamide gel electrophoresis. Then, they were transferred to PVDF membranes. Blocking with 5% skimmed milk powder (BD Pharmingen) for 1 h at room temperature, the membranes were incubated with specific anti-Arg1 (1: 1,000), anti-TNFα (1: 500), anti-IL1β (1: 1,000), anti-TNFSF15 (1: 1,000) from Abcam, anti-p-p38 MAPK (1: 1,000), anti-p38 MAPK (1: 1,000), anti-p-JNK (1: 1,000), anti-JNK (1: 1,000), anti-p-Erk1/2 (1: 2,000), anti-Erk1/2 (1: 2,000), anti-p-Akt (1: 1,000), anti-Akt (1: 1,000), anti-p-STAT1 (1: 1,000), anti-STAT1 (1: 1,000), anti-p-STAT6 (1: 1,000), anti-STAT6 (1: 1,000), anti-p-STAT3 (1: 1,000), anti-STAT3 (1: 1,000), anti-GADPH (1: 2,000) from Cell Signaling Technology, anti-iNOS (1: 1,000, Introvigen) or anti-β-actin (1: 2,000, ZSGB-BIO) overnight at 4°C. Then the membranes were washed and incubated with horseradish peroxidase-conjugated secondary antibodies. Then, protein bands were visualized by ECL Western blot reagent (Bioworld Technology).
3
Protein Extraction and Western Blot Analysis
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The total or nuclear proteins (n = 3) were extracted using commercially available kits according to the manufacturer’s protocol. Protein concentration was determined using the BCA protein assay kit (Beyotime institute of Biotechnology, China). Protein samples were analyzed using SDS-polyacrylamide gel electrophoresis (PAGE) followed by semi-dry transfer onto a PVDF membrane. The blots were then blocked with 5% nonfat milk and incubated with the appropriate primary antibodies, followed by incubation with a secondary antibody conjugated to alkaline phosphatase (Zhongshan Golden Bridge Biotechnology Co., Beijing, China). Immunoreactive bands were detected using a BCIP-NBT kit (Promega, Madison, WI, USA). The primary antibodies used were anti-VCAM-1 from Santa Cruz Biotechnology, Inc. (CA, USA), anti-MCP-1 from Boster Biological Technology, Ltd. (Wuhan, China), and anti-NF-κB, anti-inhibitory κBα, anti-p-JNK and anti-p-p38 MAPK from Abcam (Cambridge, UK).
4
CXCR4, Pim-1, and STAT5 Expression in Leukemia
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The protein expression levels of CXCR4, Pim-1, and STAT5/p-STAT5 in the experimental and control groups and the expression levels of p38 mitogen-activated protein kinase (MAPK)/p-p38 MAPK in MV4-11 and HL-60 cells incubated with different concentrations of AMD3100 (500 ng/ml, 1 μg/ml, 5 μg/ml, and 10 μg/ml) for 48 h were analyzed by Western blotting using standardized protocols. The following antibodies were used: anti-CXCR4 (BD Pharmingen, USA), anti-Pim-1 (Cell Signaling, USA), anti-STAT5 (Abcam, UK), anti-phospho (p)-STAT5 (Y694) (Abcam, UK), anti-p38 MAPK (Abcam, UK) and anti-p-p38 MAPK (Thr180/Tyr182) monoclonal antibodies (Abcam, UK).
5
Protein Expression Analysis of Exosome-Treated Cells
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After coincubation with TNIL-exos, TL-exos and A549-exos for 24 h, the total protein was extracted from cells, and the same amount of protein was electrophoresed on SDS-PAGE. A PVDF membrane was used to transfer the protein, followed by incubation in 5% skim milk to block for 1 h. Then, the primary antibodies anti-p38 MAPK (1:1000, Abcam), anti-pp38 MAPK (1:1000, Abcam), anti-HSP70 (1:1000, Abcam) and anti-HMGB1 (1:1000, Abcam) were added and incubated at 4°C overnight. Tubulin (1:1000, Abcam) served as the internal controls. The goat anti-rabbit and goat anti-mouse antibodies (1:1000, Solarbio) conjugated with HRP were used as secondary antibodies. Chemiluminescence detection was performed using the Amersham Imager 600 Imaging System.
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